Phosphorus-Containing Polymeric Zwitterion: A Pioneering
Bioresponsive Probe for P-31-Magnetic Resonance Imaging

J 2022

Phosphorus-Containing Polymeric Zwitterion: A Pioneering Bioresponsive Probe for P-31-Magnetic Resonance Imaging

KRACIKOVA, Lucie, Natalia ZIOLKOWSKA, Ladislav ANDROVIC, Iveta KLIMANKOVA, David CERVENY et. al.

Basic information

Original name

Phosphorus-Containing Polymeric Zwitterion: A Pioneering Bioresponsive Probe for P-31-Magnetic Resonance Imaging

Authors

KRACIKOVA, Lucie, Natalia ZIOLKOWSKA, Ladislav ANDROVIC, Iveta KLIMANKOVA, David CERVENY, Martin VIT, Petr POMPACH, Rafal KONEFAL, Olga JANOUSKOVA, Martin HRUBY, Daniel JIRAK and Richard LAGA

Edition

Macromolecular Bioscience, WEINHEIM, Wiley-VCH, 2022, 1616-5187

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.600

RIV identification code

RIV/00216224:14740/22:00128771

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1002/mabi.202100523

UT WoS

000766947400001

Keywords in English

P-31-MRI; MRI probes; polymer zwitterions; RAFT polymerization; ROS detection

Abstract

V originále

P-31-magnetic resonance (MR) is an important diagnostic technique currently used for tissue metabolites assessing, but it also has great potential for visualizing the internal body structures. However, due to the low physiological level of phosphorus-containing biomolecules, precise imaging requires the administration of an exogenous probe. Herein, this work describes the synthesis and MR characterization of a pioneering metal-free P-31-MR probe based on phosphorus-containing polymeric zwitterion. The developed probe (pTMPC) is a well-defined water-soluble macromolecule characterized by a high content of naturally rare phosphorothioate groups providing a high-intensity P-31-MR signal clearly distinguishable from biological background both in vitro and in vitro. In addition, pTMPC can serve as a sensitive P-31-MR sensor of pathological conditions in vivo because it undergoes oxidation-induced structural changes in the presence of reactive oxygen species (ROS). Add to this the favorable H-1 and P-31 T-1/T-2 relaxation times and biocompatibility, pTMPC represents a conceptually new diagnostic, whose discovery opens up new possibilities in the field of P-31-MR spectroscopy and imaging.

Links

90127, large research infrastructures

Name: CIISB II

Citovat

KRACIKOVA, Lucie, Natalia ZIOLKOWSKA, Ladislav ANDROVIC, Iveta KLIMANKOVA, David CERVENY, Martin VIT, Petr POMPACH, Rafal KONEFAL, Olga JANOUSKOVA, Martin HRUBY, Daniel JIRAK and Richard LAGA. Phosphorus-Containing Polymeric Zwitterion: A Pioneering Bioresponsive Probe for P-31-Magnetic Resonance Imaging. Macromolecular Bioscience. WEINHEIM: Wiley-VCH, 2022, vol. 22, No 5, p. 2100523-2100532. ISSN 1616-5187. Available from: https://dx.doi.org/10.1002/mabi.202100523.

@article{2262445,
   author = {Kracikova, Lucie and Ziolkowska, Natalia and Androvic, Ladislav and Klimankova, Iveta and Cerveny, David and Vit, Martin and Pompach, Petr and Konefal, Rafal and Janouskova, Olga and Hruby, Martin and Jirak, Daniel and Laga, Richard},
   article_location = {WEINHEIM},
   article_number = {5},
   doi = {http://dx.doi.org/10.1002/mabi.202100523},
   keywords = {P-31-MRI; MRI probes; polymer zwitterions; RAFT polymerization; ROS detection},
   language = {eng},
   issn = {1616-5187},
   journal = {Macromolecular Bioscience},
   title = {Phosphorus-Containing Polymeric Zwitterion: A Pioneering Bioresponsive Probe for P-31-Magnetic Resonance Imaging},
   url = {https://onlinelibrary.wiley.com/doi/10.1002/mabi.202100523},
   volume = {22},
   year = {2022}
}

TY  - JOUR
ID  - 2262445
AU  - Kracikova, Lucie - Ziolkowska, Natalia - Androvic, Ladislav - Klimankova, Iveta - Cerveny, David - Vit, Martin - Pompach, Petr - Konefal, Rafal - Janouskova, Olga - Hruby, Martin - Jirak, Daniel - Laga, Richard
PY  - 2022
TI  - Phosphorus-Containing Polymeric Zwitterion: A Pioneering Bioresponsive Probe for P-31-Magnetic Resonance Imaging
JF  - Macromolecular Bioscience
VL  - 22
IS  - 5
SP  - 2100523
EP  - 2100523
PB  - Wiley-VCH
SN  - 16165187
KW  - P-31-MRI
KW  - MRI probes
KW  - polymer zwitterions
KW  - RAFT polymerization
KW  - ROS detection
UR  - https://onlinelibrary.wiley.com/doi/10.1002/mabi.202100523
N2  - P-31-magnetic resonance (MR) is an important diagnostic technique currently used for tissue metabolites assessing, but it also has great potential for visualizing the internal body structures. However, due to the low physiological level of phosphorus-containing biomolecules, precise imaging requires the administration of an exogenous probe. Herein, this work describes the synthesis and MR characterization of a pioneering metal-free P-31-MR probe based on phosphorus-containing polymeric zwitterion. The developed probe (pTMPC) is a well-defined water-soluble macromolecule characterized by a high content of naturally rare phosphorothioate groups providing a high-intensity P-31-MR signal clearly distinguishable from biological background both in vitro and in vitro. In addition, pTMPC can serve as a sensitive P-31-MR sensor of pathological conditions in vivo because it undergoes oxidation-induced structural changes in the presence of reactive oxygen species (ROS). Add to this the favorable H-1 and P-31 T-1/T-2 relaxation times and biocompatibility, pTMPC represents a conceptually new diagnostic, whose discovery opens up new possibilities in the field of P-31-MR spectroscopy and imaging.
ER  -

KRACIKOVA, Lucie, Natalia ZIOLKOWSKA, Ladislav ANDROVIC, Iveta KLIMANKOVA, David CERVENY, Martin VIT, Petr POMPACH, Rafal KONEFAL, Olga JANOUSKOVA, Martin HRUBY, Daniel JIRAK and Richard LAGA. Phosphorus-Containing Polymeric Zwitterion: A Pioneering Bioresponsive Probe for P-31-Magnetic Resonance Imaging. \textit{Macromolecular Bioscience}. WEINHEIM: Wiley-VCH, 2022, vol.~22, No~5, p.~2100523-2100532. ISSN~1616-5187. Available from: https://dx.doi.org/10.1002/mabi.202100523.

Detailed Information on Publication Record