Other formats:
BibTeX
LaTeX
RIS
@article{2262558,
author = {Melvin, Emelia and Kalaninova, Zuzana and Shlush, Elia and Man, Petr and Giladi, Moshe and Haitin, Yoni},
article_number = {1},
doi = {http://dx.doi.org/10.1038/s42003-022-03862-3},
keywords = {Animals; Cell Membrane; Detergents; Hydrogen Deuterium Exchange-Mass Spectrometry; Mice},
language = {eng},
issn = {2399-3642},
journal = {Communications Biology},
title = {TTYH family members form tetrameric complexes at the cell membrane},
url = {https://www.nature.com/articles/s42003-022-03862-3},
volume = {5},
year = {2022}
}
TY - JOUR
ID - 2262558
AU - Melvin, Emelia - Kalaninova, Zuzana - Shlush, Elia - Man, Petr - Giladi, Moshe - Haitin, Yoni
PY - 2022
TI - TTYH family members form tetrameric complexes at the cell membrane
JF - Communications Biology
VL - 5
IS - 1
SP - 886
EP - 886
PB - Nature Research
SN - 23993642
KW - Animals
KW - Cell Membrane
KW - Detergents
KW - Hydrogen Deuterium Exchange-Mass Spectrometry
KW - Mice
UR - https://www.nature.com/articles/s42003-022-03862-3
N2 - Single-molecule microscopy and in situ cross-linking show that the conserved Tweety homolog (TTYH) proteins have an innate tetrameric organization at the cell membrane. The conserved Tweety homolog (TTYH) family consists of three paralogs in vertebrates, displaying a ubiquitous expression pattern. Although considered as ion channels for almost two decades, recent structural and functional analyses refuted this role. Intriguingly, while all paralogs shared a dimeric stoichiometry following detergent solubilization, their structures revealed divergence in their relative subunit orientation. Here, we determined the stoichiometry of intact mouse TTYH (mTTYH) complexes in cells. Using cross-linking and single-molecule fluorescence microscopy, we demonstrate that mTTYH1 and mTTYH3 form tetramers at the plasma membrane, stabilized by interactions between their extracellular domains. Using blue-native PAGE, fluorescence-detection size-exclusion chromatography, and hydrogen/deuterium exchange mass spectrometry (HDX-MS), we reveal that detergent solubilization results in tetramers destabilization, leading to their dissolution into dimers. Moreover, HDX-MS demonstrates that the extracellular domains are stabilized in the context of the tetrameric mTTYH complex. Together, our results expose the innate tetrameric organization of TTYH complexes at the cell membrane. Future structural analyses of these assemblies in native membranes are required to illuminate their long-sought cellular function.
ER -
MELVIN, Emelia, Zuzana KALANINOVA, Elia SHLUSH, Petr MAN, Moshe GILADI and Yoni HAITIN. TTYH family members form tetrameric complexes at the cell membrane. \textit{Communications Biology}. Nature Research, 2022, vol.~5, No~1, p.~886-896. ISSN~2399-3642. Available from: https://dx.doi.org/10.1038/s42003-022-03862-3.
|
