Dual specificity phosphatase 7 drives the formation of cardiac mesoderm in mouse embryonic stem cells

SLADEČEK, Stanislava, Katarzyna Anna RADASZKIEWICZ, Martina BŐHMOVÁ, Tomáš GYBEĽ, Tomasz Witold RADASZKIEWICZ and Jiří PACHERNÍK. Dual specificity phosphatase 7 drives the formation of cardiac mesoderm in mouse embryonic stem cells. Plos one. San Francisco: Public Library of Science, 2022, vol. 17, No 10, p. 1-22. ISSN 1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0275860.

Basic information

• Original name: Dual specificity phosphatase 7 drives the formation of cardiac mesoderm in mouse embryonic stem cells
• Authors: SLADEČEK, Stanislava (703 Slovakia, belonging to the institution), Katarzyna Anna RADASZKIEWICZ (616 Poland, belonging to the institution), Martina BŐHMOVÁ (203 Czech Republic, belonging to the institution), Tomáš GYBEĽ (703 Slovakia, belonging to the institution), Tomasz Witold RADASZKIEWICZ (616 Poland, belonging to the institution) and Jiří PACHERNÍK (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Plos one, San Francisco, Public Library of Science, 2022, 1932-6203.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10601 Cell biology
• Country of publisher: United States of America
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 3.700
• RIV identification code: RIV/00216224:14310/22:00128793
• Organization unit: Faculty of Science
• Doi: http://dx.doi.org/10.1371/journal.pone.0275860
• UT WoS: 000924819300068
• Keywords in English: Cell differentiation; Cardiomyocytes; Cell staining; Heart; Mesoderm; Small interfering RNA; Phosphorylation; MAPK signaling cascades
• Tags: rivok
• Tags: International impact, Reviewed

Abstract

Dual specificity phosphatase 7 (DUSP7) is a protein belonging to a broad group of phosphatases that can dephosphorylate phosphoserine/phosphothreonine as well as phosphotyrosine residues within the same substrate. DUSP7 has been linked to the negative regulation of mitogen activated protein kinases (MAPK), and in particular to the regulation of extracellular signal-regulated kinases 1 and 2 (ERK1/2). MAPKs play an important role in embryonic development, where their duration, magnitude, and spatiotemporal activity must be strictly controlled by other proteins, among others by DUSPs. In this study, we focused on the effect of DUSP7 depletion on the in vitro differentiation of mouse embryonic stem (ES) cells. We showed that even though DUSP7 knock-out ES cells do retain some of their basic characteristics, when it comes to differentiation, they preferentially differentiate towards neural cells, while the formation of early cardiac mesoderm is repressed. Therefore, our data indicate that DUSP7 is necessary for the correct formation of neuroectoderm and cardiac mesoderm during the in vitro differentiation of ES cells.

Links

• GA18-18235S, research and development project: Name: Nové možnosti v kardiomyogenní diferenciaci pluripotentních kmenových buněk (Acronym: KarPKB)

Investor: Czech Science Foundation

• GA19-16861S, research and development project: Name: Interakce biomateriálů s kmenovými buňkami v simulovaných in vivo podmínkách (Acronym: IBMSKB)

Investor: Czech Science Foundation

• MUNI/A/1145/2017, interní kód MU: Name: Podpora výzkumné činnosti studentů v oblasti fyziologie, vývojové biologie a imunologie živočichů v roce 2018 (Acronym: OFIŽ 2018)

Investor: Masaryk University, Category A