| FEDOROVÁ, Veronika, Veronika POSPÍŠILOVÁ, Tereza VÁŇOVÁ, Kateřina AMRUZ ČERNÁ, Pavel ABAFFY, Jiří SEDMÍK, Jan RAŠKA, Simona VOCHYÁNOVÁ, Zuzana MATŮŠOVÁ, Jana HOUSEROVÁ, Lukáš VALIHRACH, Zdeněk HODNÝ and Dáša BOHAČIAKOVÁ. Glioblastoma and cerebral organoids: development and analysis of an in vitro model for glioblastoma migration. MOLECULAR ONCOLOGY. ENGLAND: WILEY, 2023, vol. 17, No 4, p. 647-663. ISSN 1574-7891. Available from: https://dx.doi.org/10.1002/1878-0261.13389. |
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@article{2263677,
author = {Fedorová, Veronika and Pospíšilová, Veronika and Váňová, Tereza and Amruz Černá, Kateřina and Abaffy, Pavel and Sedmík, Jiří and Raška, Jan and Vochyánová, Simona and Matůšová, Zuzana and Houserová, Jana and Valihrach, Lukáš and Hodný, Zdeněk and Bohačiaková, Dáša},
article_location = {ENGLAND},
article_number = {4},
doi = {http://dx.doi.org/10.1002/1878-0261.13389},
keywords = {cerebral organoids; GLICO; glioblastoma; induced pluripotent stem cells},
language = {eng},
issn = {1574-7891},
journal = {MOLECULAR ONCOLOGY},
title = {Glioblastoma and cerebral organoids: development and analysis of an in vitro model for glioblastoma migration},
url = {https://febs.onlinelibrary.wiley.com/doi/10.1002/1878-0261.13389},
volume = {17},
year = {2023}
}
TY - JOUR
ID - 2263677
AU - Fedorová, Veronika - Pospíšilová, Veronika - Váňová, Tereza - Amruz Černá, Kateřina - Abaffy, Pavel - Sedmík, Jiří - Raška, Jan - Vochyánová, Simona - Matůšová, Zuzana - Houserová, Jana - Valihrach, Lukáš - Hodný, Zdeněk - Bohačiaková, Dáša
PY - 2023
TI - Glioblastoma and cerebral organoids: development and analysis of an in vitro model for glioblastoma migration
JF - MOLECULAR ONCOLOGY
VL - 17
IS - 4
SP - 647-663
EP - 647-663
PB - WILEY
SN - 15747891
KW - cerebral organoids
KW - GLICO
KW - glioblastoma
KW - induced pluripotent stem cells
UR - https://febs.onlinelibrary.wiley.com/doi/10.1002/1878-0261.13389
N2 - It is currently challenging to adequately model the growth and migration of glioblastoma using two-dimensional (2D) in vitro culture systems as they quickly lose the original, patient-specific identity and heterogeneity. However, with the advent of three-dimensional (3D) cell cultures and human-induced pluripotent stem cell (iPSC)-derived cerebral organoids (COs), studies demonstrate that the glioblastoma-CO (GLICO) coculture model helps to preserve the phenotype of the patient-specific tissue. Here, we aimed to set up such a model using mature COs and develop a pipeline for subsequent analysis of cocultured glioblastoma. Our data demonstrate that the growth and migration of the glioblastoma cell line within the mature COs are significantly increased in the presence of extracellular matrix proteins, shortening the time needed for glioblastoma to initiate migration. We also describe in detail the method for the visualization and quantification of these migrating cells within the GLICO model. Lastly, we show that this coculture model (and the human brain-like microenvironment) can significantly transform the gene expression profile of the established U87 glioblastoma cell line into proneural and classical glioblastoma cell types.
ER -
FEDOROVÁ, Veronika, Veronika POSPÍŠILOVÁ, Tereza VÁŇOVÁ, Kateřina AMRUZ ČERNÁ, Pavel ABAFFY, Jiří SEDMÍK, Jan RAŠKA, Simona VOCHYÁNOVÁ, Zuzana MATŮŠOVÁ, Jana HOUSEROVÁ, Lukáš VALIHRACH, Zdeněk HODNÝ and Dáša BOHAČIAKOVÁ. Glioblastoma and cerebral organoids: development and analysis of an in vitro model for glioblastoma migration. \textit{MOLECULAR ONCOLOGY}. ENGLAND: WILEY, 2023, vol.~17, No~4, p.~647-663. ISSN~1574-7891. Available from: https://dx.doi.org/10.1002/1878-0261.13389.
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