KOMÁREK, Jan, Marek KORSÁK, Lukáš FALTINEK and Michaela WIMMEROVÁ. Development of lectin arrays for mapping the glycosylation changes in proteins and cells. In XXIst Meeting of Biochemists and Molecular Biologists. 2022. ISBN 978-80-280-0136-0.
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Basic information
Original name Development of lectin arrays for mapping the glycosylation changes in proteins and cells
Authors KOMÁREK, Jan (203 Czech Republic, belonging to the institution), Marek KORSÁK (703 Slovakia, belonging to the institution), Lukáš FALTINEK (203 Czech Republic, belonging to the institution) and Michaela WIMMEROVÁ (203 Czech Republic, guarantor, belonging to the institution).
Edition XXIst Meeting of Biochemists and Molecular Biologists, 2022.
Other information
Original language English
Type of outcome Conference abstract
Field of Study 10608 Biochemistry and molecular biology
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/22:00129422
Organization unit Faculty of Science
ISBN 978-80-280-0136-0
Keywords (in Czech) lektiny, glykosylace, lektinové čipy
Keywords in English lectins; glycosylation; lectin arrays
Changed by Changed by: Mgr. Jan Komárek, Ph.D., učo 175127. Changed: 6/3/2023 14:13.
Abstract
Saccharides are essential for life, and in addition to their traditional roles as structural and energy storage components, they play crucial roles in many recognition events, signaling, and communication processes. The glycosylation of cells reflects the biological species, tissue, and physiological state of the organism. Importantly, changes in glycosylation patterns were observed in various tumors, and the aberrant glycosylation is often associated with malignancy potential, tumor immune surveillance, and patients´ prognosis. Lectin arrays are widely used tools for analyzing glycosylation in proteins and cells. They utilize a panel of lectins (saccharide-binding proteins with distinct sugar specificities) immobilized onto a solid surface. Lectin arrays allow for rapid and high-throughput glycomic analysis with minimal sample consumption. They can be used for glycan profiling of various samples, including purified glycoproteins, cell/tissue lysates, membrane vesicles, complex biological samples, or entire cells. This talk will present the development of customized lectin arrays in two different formats. The classical array in the microscope slide format was prepared by non-contact piezo dispensing of lectins onto a functionalized glass slide and their immobilization using various surface chemistries (immobilization on epoxy groups, NHS/EDC coupling). In the slide format, binding of glycosylated samples was detected by fluorescence measurement. To allow label-free detection of attached glyco-samples, lectin microarray prepared on bio-layer interferometry (BLI) sensor tips is currently being optimized. The fabricated lectin arrays will be used to map the glycosylation changes at different stages of cell differentiation and under different physiopathological conditions.
Links
GA21-29622S, research and development projectName: Biologická úloha lektinů v symbióze a patogenezi
Investor: Czech Science Foundation
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