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@proceedings{2265428, author = {Svojanovský, Vilém and Stiborek, Marek and Stráník, Jaromír and Jindřichová, Lenka and Krásenský, Pavel and Navrátilová, Jarmila and Adamová, Barbora and Kanický, Viktor and Preisler, Jan}, booktitle = {EWCPS - European Winter Conference on Plasma Spectrochemistry}, keywords = {ICP MS; single particle; NP; laser ablation; LA}, language = {eng}, title = {Single particle inductively coupled plasma mass spectrometry imaging of immunochemically labeled spheroid sections}, year = {2023} }
TY - CONF ID - 2265428 AU - Svojanovský, Vilém - Stiborek, Marek - Stráník, Jaromír - Jindřichová, Lenka - Krásenský, Pavel - Navrátilová, Jarmila - Adamová, Barbora - Kanický, Viktor - Preisler, Jan PY - 2023 TI - Single particle inductively coupled plasma mass spectrometry imaging of immunochemically labeled spheroid sections KW - ICP MS KW - single particle KW - NP KW - laser ablation KW - LA N2 - Spheroids are one of the 3D biological models in which the arrangement of proliferating and necrotic cells resembles the tumor microenvironment. One of the markers of proliferation is Ki-67 protein, which is expressed by cells in the active phase of the cell cycle. Immunohistochemical labeling with a primary antibody and a fluorophore-labeled secondary antibody followed by fluorescence microscopy analysis is commonly employed for two- and three-dimensional imaging of the target protein in spheroid sections. We have successfully switched from fluorophore labeling of Ki-67 to nanoparticle labeling combined with LA ICP MS.* Recently, gold nanoparticles were used as very sensitive immunochemical tags in conventional UV laser ablation ICP MSI.1,2 In our approach, intact 20 nm gold nanoparticles tags were desorbed from the spheroid section with 2 940 nm laser ablation system. ER -
SVOJANOVSKÝ, Vilém, Marek STIBOREK, Jaromír STRÁNÍK, Lenka JINDŘICHOVÁ, Pavel KRÁSENSKÝ, Jarmila NAVRÁTILOVÁ, Barbora ADAMOVÁ, Viktor KANICKÝ a Jan PREISLER. Single particle inductively coupled plasma mass spectrometry imaging of immunochemically labeled spheroid sections. In \textit{EWCPS - European Winter Conference on Plasma Spectrochemistry}. 2023.
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