| PREISLER, Jan, Marek STIBOREK, Lenka JINDŘICHOVÁ, Stanislava MELIORISOVÁ, Antonín BEDNAŘÍK, Vadym PRYSIAZHNYI, Jiří KROUPA, Pavel HOUŠKA, Barbora ADAMOVÁ, Jarmila NAVRÁTILOVÁ and Viktor KANICKÝ. Nanoparticle tag counting for tissue imaging using infrared laser ablation. In APCE-CECE-ITP-IUPAC 2022. 2022. |
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@proceedings{2265500,
author = {Preisler, Jan and Stiborek, Marek and Jindřichová, Lenka and Meliorisová, Stanislava and Bednařík, Antonín and Prysiazhnyi, Vadym and Kroupa, Jiří and Houška, Pavel and Adamová, Barbora and Navrátilová, Jarmila and Kanický, Viktor},
booktitle = {APCE-CECE-ITP-IUPAC 2022},
keywords = {mass spectrometry imaging; gold nanoparticles; laser ablation; single particle inductively coupled plasma mass spectrometry; single molecule imaging},
language = {eng},
title = {Nanoparticle tag counting for tissue imaging using infrared laser ablation},
year = {2022}
}
TY - CONF
ID - 2265500
AU - Preisler, Jan - Stiborek, Marek - Jindřichová, Lenka - Meliorisová, Stanislava - Bednařík, Antonín - Prysiazhnyi, Vadym - Kroupa, Jiří - Houška, Pavel - Adamová, Barbora - Navrátilová, Jarmila - Kanický, Viktor
PY - 2022
TI - Nanoparticle tag counting for tissue imaging using infrared laser ablation
KW - mass spectrometry imaging
KW - gold nanoparticles
KW - laser ablation
KW - single particle inductively coupled plasma mass spectrometry
KW - single molecule imaging
N2 - We report a new laser ablation technique employing a pulsed 2 940 nm laser and a simple ablation cell for digital mapping biomarkers in tissues using metal nanoparticle tags. Unlike the conventional laser ablation systems, where nanoparticles are ablated, we are able to desorb intact nanoparticles and count them. The method is demonstrated on monitoring proliferating cells in 3D aggregates of human colorectal carcinoma cells. Precise counting of the tags in each pixel generates sharp distribution maps of a proliferation biomarker in the tissue. A mass spectrometer detects up to 83% nanoparticles of a selected element with a single particle detection limit. Advantageously, the desorption of nanoparticles from regions outside the tissue is strongly suppressed.
ER -
PREISLER, Jan, Marek STIBOREK, Lenka JINDŘICHOVÁ, Stanislava MELIORISOVÁ, Antonín BEDNAŘÍK, Vadym PRYSIAZHNYI, Jiří KROUPA, Pavel HOUŠKA, Barbora ADAMOVÁ, Jarmila NAVRÁTILOVÁ and Viktor KANICKÝ. Nanoparticle tag counting for tissue imaging using infrared laser ablation. In \textit{APCE-CECE-ITP-IUPAC 2022}. 2022.
|
