Nanoparticle tag counting for tissue imaging using infrared
laser ablation

a 2022

Nanoparticle tag counting for tissue imaging using infrared laser ablation

PREISLER, Jan, Marek STIBOREK, Lenka JINDŘICHOVÁ, Stanislava MELIORISOVÁ, Antonín BEDNAŘÍK et. al.

Basic information

Original name

Nanoparticle tag counting for tissue imaging using infrared laser ablation

Authors

PREISLER, Jan (203 Czech Republic, guarantor, belonging to the institution), Marek STIBOREK (203 Czech Republic), Lenka JINDŘICHOVÁ (203 Czech Republic), Stanislava MELIORISOVÁ (703 Slovakia), Antonín BEDNAŘÍK (203 Czech Republic), Vadym PRYSIAZHNYI (804 Ukraine), Jiří KROUPA (203 Czech Republic), Pavel HOUŠKA (203 Czech Republic), Barbora ADAMOVÁ (203 Czech Republic), Jarmila NAVRÁTILOVÁ (203 Czech Republic) and Viktor KANICKÝ (203 Czech Republic)

Edition

APCE-CECE-ITP-IUPAC 2022, 2022

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10406 Analytical chemistry

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/22:00128826

Organization unit

Faculty of Science

Keywords in English

mass spectrometry imaging; gold nanoparticles; laser ablation; single particle inductively coupled plasma mass spectrometry; single molecule imaging

Změněno: 8/3/2023 13:29, prof. Mgr. Jan Preisler, Ph.D.

Abstract

V originále

We report a new laser ablation technique employing a pulsed 2 940 nm laser and a simple ablation cell for digital mapping biomarkers in tissues using metal nanoparticle tags. Unlike the conventional laser ablation systems, where nanoparticles are ablated, we are able to desorb intact nanoparticles and count them. The method is demonstrated on monitoring proliferating cells in 3D aggregates of human colorectal carcinoma cells. Precise counting of the tags in each pixel generates sharp distribution maps of a proliferation biomarker in the tissue. A mass spectrometer detects up to 83% nanoparticles of a selected element with a single particle detection limit. Advantageously, the desorption of nanoparticles from regions outside the tissue is strongly suppressed.

Links

GA21-12262S, research and development project

Name: Nanočásticové sondy pro zobrazovací hmotnostní spektrometrii

Investor: Czech Science Foundation

LX22NPO5102, research and development project

Name: Národní ústav pro výzkum rakoviny (Acronym: NÚVR)

Investor: Ministry of Education, Youth and Sports of the CR, National institute for cancer research, 5.1 EXCELES

MUNI/A/1325/2021, interní kód MU

Name: Podpora výzkumné činnosti studentů molekulární biologie a genetiky 10 (Acronym: MBG10)

Investor: Masaryk University

Citovat

PREISLER, Jan, Marek STIBOREK, Lenka JINDŘICHOVÁ, Stanislava MELIORISOVÁ, Antonín BEDNAŘÍK, Vadym PRYSIAZHNYI, Jiří KROUPA, Pavel HOUŠKA, Barbora ADAMOVÁ, Jarmila NAVRÁTILOVÁ and Viktor KANICKÝ. Nanoparticle tag counting for tissue imaging using infrared laser ablation. In APCE-CECE-ITP-IUPAC 2022. 2022.

@proceedings{2265500,
   author = {Preisler, Jan and Stiborek, Marek and Jindřichová, Lenka and Meliorisová, Stanislava and Bednařík, Antonín and Prysiazhnyi, Vadym and Kroupa, Jiří and Houška, Pavel and Adamová, Barbora and Navrátilová, Jarmila and Kanický, Viktor},
   booktitle = {APCE-CECE-ITP-IUPAC 2022},
   keywords = {mass spectrometry imaging; gold nanoparticles; laser ablation; single particle inductively coupled plasma mass spectrometry; single molecule imaging},
   language = {eng},
   title = {Nanoparticle tag counting for tissue imaging using infrared laser ablation},
   year = {2022}
}

TY  - CONF
ID  - 2265500
AU  - Preisler, Jan - Stiborek, Marek - Jindřichová, Lenka - Meliorisová, Stanislava - Bednařík, Antonín - Prysiazhnyi, Vadym - Kroupa, Jiří - Houška, Pavel - Adamová, Barbora - Navrátilová, Jarmila - Kanický, Viktor
PY  - 2022
TI  - Nanoparticle tag counting for tissue imaging using infrared laser ablation
KW  - mass spectrometry imaging
KW  - gold nanoparticles
KW  - laser ablation
KW  - single particle inductively coupled plasma mass spectrometry
KW  - single molecule imaging
N2  - We report a new laser ablation technique employing a pulsed 2 940 nm laser and a simple ablation cell for digital mapping biomarkers in tissues using metal nanoparticle tags. Unlike the conventional laser ablation systems, where nanoparticles are ablated, we are able to desorb intact nanoparticles and count them. The method is demonstrated on monitoring proliferating cells in 3D aggregates of human colorectal carcinoma cells. Precise counting of the tags in each pixel generates sharp distribution maps of a proliferation biomarker in the tissue. A mass spectrometer detects up to 83% nanoparticles of a selected element with a single particle detection limit. Advantageously, the desorption of nanoparticles from regions outside the tissue is strongly suppressed.
ER  -

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