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@article{2266098,
author = {Vymazalová, Kateřina and Šerý, Omar and Králík, Petr and Dziedzinská, Radka and Musilová, Zuzana and Frišhons, Jan and Vojtíšek, Tomáš and Joukal, Marek},
article_location = {NEW YORK},
article_number = {3},
doi = {http://dx.doi.org/10.1007/s12565-023-00707-9},
keywords = {Embalming; Dissection; Infections; Coronavirus; Polymerase chain reaction},
language = {eng},
issn = {1447-6959},
journal = {Anatomical Science International},
title = {Substantial decrease in SARS-CoV-2 RNA after fixation of cadavers intended for anatomical dissection},
url = {https://link.springer.com/article/10.1007/s12565-023-00707-9},
volume = {98},
year = {2023}
}
TY - JOUR
ID - 2266098
AU - Vymazalová, Kateřina - Šerý, Omar - Králík, Petr - Dziedzinská, Radka - Musilová, Zuzana - Frišhons, Jan - Vojtíšek, Tomáš - Joukal, Marek
PY - 2023
TI - Substantial decrease in SARS-CoV-2 RNA after fixation of cadavers intended for anatomical dissection
JF - Anatomical Science International
VL - 98
IS - 3
SP - 441-447
EP - 441-447
PB - SPRINGER
SN - 14476959
KW - Embalming
KW - Dissection
KW - Infections
KW - Coronavirus
KW - Polymerase chain reaction
UR - https://link.springer.com/article/10.1007/s12565-023-00707-9
N2 - With the onset of the COVID-19 pandemic, a problem arose with classic body donation programmes for obtaining cadavers for anatomical dissections, science and research. The question has emerged whether bodies of individuals who died of COVID-19 or were infected by SARS-CoV-2 could be admitted to Departments of Anatomy. To determine the risk of SARS-CoV-2 transmission to employees or students, the presence and stability of SARS-CoV-2 RNA in cadavers after fixation agents’ application and subsequent post-fixation baths over time were examined. The presence of viral RNA in swabs from selected tissues was assessed by the standardized routine RNA isolation protocol and subsequent real-time PCR analysis. To support the results obtained from the tissue swabs, samples of RNA were exposed in vitro to short and long-term exposure to the components of the injection and fixation solutions used for the bodies’ conservation. Substantial removal of SARS-CoV-2 RNA was observed in post-mortem tissue following perfusion with 3.5% phenol, 2.2% formaldehyde, 11.8% glycerol and 55% ethanol, and subsequent post-fixation in an ethanol bath. In vitro experiments showed significant effects of formaldehyde on SARS-CoV-2 RNA, while phenol and ethanol showed only negligible effects. We conclude that cadavers subjected to fixation protocols as described here should not pose a considerable risk of SARS-CoV-2 infection while being handled by students and staff and are, therefore, suitable for routine anatomical dissections and teaching.
ER -
VYMAZALOVÁ, Kateřina, Omar ŠERÝ, Petr KRÁLÍK, Radka DZIEDZINSKÁ, Zuzana MUSILOVÁ, Jan FRIŠHONS, Tomáš VOJTÍŠEK and Marek JOUKAL. Substantial decrease in SARS-CoV-2 RNA after fixation of cadavers intended for anatomical dissection. \textit{Anatomical Science International}. NEW YORK: SPRINGER, 2023, vol.~98, No~3, p.~441-447. ISSN~1447-6959. Available from: https://dx.doi.org/10.1007/s12565-023-00707-9.
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