Detailed Information on Publication Record
2022
Transcriptomic landscape of Staphylococcus aureus during phage K infection
FINSTRLOVÁ, Adéla, Ivana MAŠLAŇOVÁ, Bob BLASDEL REUTER, Jiří DOŠKAŘ, Friedrich GÖTZ et. al.Basic information
Original name
Transcriptomic landscape of Staphylococcus aureus during phage K infection
Authors
FINSTRLOVÁ, Adéla (203 Czech Republic, belonging to the institution), Ivana MAŠLAŇOVÁ (203 Czech Republic, guarantor, belonging to the institution), Bob BLASDEL REUTER (56 Belgium), Jiří DOŠKAŘ (203 Czech Republic, belonging to the institution), Friedrich GÖTZ (276 Germany) and Roman PANTŮČEK (203 Czech Republic, belonging to the institution)
Edition
Viruses of Microbes 2022, 2022
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
10607 Virology
Country of publisher
Portugal
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
RIV identification code
RIV/00216224:14310/22:00129742
Organization unit
Faculty of Science
Keywords in English
Staphylococcus; bacteriophage; phage therapy; transcriptome
Změněno: 13/3/2023 16:15, prof. RNDr. Roman Pantůček, Ph.D.
Abstract
V originále
The treatment of infections caused by human and veterinary pathogen Staphylococcus aureus is becoming worldwide healthcare concern due to the increasing resistance to antibiotics. A promising alternative to currently used drugs is represented by lytic phages from genus Kayvirus, but their use is impeded by the lack of knowledge of phage-bacterium molecular interactions. We performed RNA sequencing of two S. aureus strains infected with Kayvirus bacteriophage K to decipher the transcriptomics of the phage lytic life-cycle and the host response. We found that the temporal transcriptional profile of phage K was comparable in both strains except for a few loci. The RNA-Seq data also revealed presence of phage non-coding RNAs, which may play a role in the regulation of phage and host gene expression. The response of S. aureus to phage K infection resembles a general stress response and involves upregulation of nucleotide, amino acid and energy synthesis and transporter genes and the downregulation of host transcription factors. The interaction of phage K with variable genetic elements of the host showed slight upregulation of gene expression of prophage integrases and antirepressors. The virulence genes involved in adhesion and immune evasion were only marginally affected. The study gives a comprehensive view on the phage-bacterium interactions that improves the knowledge of molecular mechanisms underlying the Kayvirus lytic action. We clarify the global transcriptional interactions between phage and host, which will ensure safer usage of phage therapeutics and may also serve as a basis for development of new antibacterial strategies.
Links
NU22-05-00042, research and development project |
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