BRANDMEIER, Julian, Natalia JURGA, Tomasz GRZYB, Antonín HLAVÁČEK, Radka OBOŘILOVÁ, Petr SKLÁDAL, Zdeněk FARKA and Hans-Heiner GORRIS. Digital and Analog Detection of SARS-CoV-2 Nucleocapsid Protein via an Upconversion-Linked Immunosorbent Assay. Analytical Chemistry. Washington, DC: American Chemical Society, 2023, vol. 95, No 10, p. 4753-4759. ISSN 0003-2700. Available from: https://dx.doi.org/10.1021/acs.analchem.2c05670. |
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@article{2267281, author = {Brandmeier, Julian and Jurga, Natalia and Grzyb, Tomasz and Hlaváček, Antonín and Obořilová, Radka and Skládal, Petr and Farka, Zdeněk and Gorris, HansandHeiner}, article_location = {Washington, DC}, article_number = {10}, doi = {http://dx.doi.org/10.1021/acs.analchem.2c05670}, keywords = {Covid-19; SARS-CoV-2; immunoassay; photon-upconversion nanoparticle; ULISA; digital detection}, language = {eng}, issn = {0003-2700}, journal = {Analytical Chemistry}, title = {Digital and Analog Detection of SARS-CoV-2 Nucleocapsid Protein via an Upconversion-Linked Immunosorbent Assay}, url = {https://pubs.acs.org/doi/10.1021/acs.analchem.2c05670}, volume = {95}, year = {2023} }
TY - JOUR ID - 2267281 AU - Brandmeier, Julian - Jurga, Natalia - Grzyb, Tomasz - Hlaváček, Antonín - Obořilová, Radka - Skládal, Petr - Farka, Zdeněk - Gorris, Hans-Heiner PY - 2023 TI - Digital and Analog Detection of SARS-CoV-2 Nucleocapsid Protein via an Upconversion-Linked Immunosorbent Assay JF - Analytical Chemistry VL - 95 IS - 10 SP - 4753-4759 EP - 4753-4759 PB - American Chemical Society SN - 00032700 KW - Covid-19 KW - SARS-CoV-2 KW - immunoassay KW - photon-upconversion nanoparticle KW - ULISA KW - digital detection UR - https://pubs.acs.org/doi/10.1021/acs.analchem.2c05670 N2 - The COVID-19 crisis requires fast and highly sensitive tests for the early stage detection of the SARS-CoV-2 virus. For detecting the nucleocapsid protein (N protein), the most abundant viral antigen, we have employed upconversion nanoparticles that emit short-wavelength light under near-infrared excitation (976 nm). The anti-Stokes emission avoids autofluorescence and light scattering and thus enables measurements without optical background interference. The sandwich upconversion-linked immunosorbent assay (ULISA) can be operated both in a conventional analog mode and in a digital mode based on counting individual immune complexes. We have investigated how different antibody combinations affect the detection of the wildtype N protein and the detection of SARS-CoV-2 (alpha variant) in lysed culture fluid via the N protein. The ULISA yielded a limit of detection (LOD) of 1.3 pg/mL (27 fM) for N protein detection independent of the analog or digital readout, which is approximately 3 orders of magnitude more sensitive than conventional enzyme-linked immunosorbent assays or commercial lateral flow assays for home testing. In the case of SARS-CoV-2, the digital ULISA additionally improved the LOD by a factor of 10 compared to the analog readout. ER -
BRANDMEIER, Julian, Natalia JURGA, Tomasz GRZYB, Antonín HLAVÁČEK, Radka OBOŘILOVÁ, Petr SKLÁDAL, Zdeněk FARKA and Hans-Heiner GORRIS. Digital and Analog Detection of SARS-CoV-2 Nucleocapsid Protein via an Upconversion-Linked Immunosorbent Assay. \textit{Analytical Chemistry}. Washington, DC: American Chemical Society, 2023, vol.~95, No~10, p.~4753-4759. ISSN~0003-2700. Available from: https://dx.doi.org/10.1021/acs.analchem.2c05670.
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