Antibiotic Susceptibility, Resistance Gene Determinants and
Corresponding Genomic Regions in Lactobacillus amylovorus
Isolates Derived from Wild Boars and Domestic Pigs

J 2023

Antibiotic Susceptibility, Resistance Gene Determinants and Corresponding Genomic Regions in Lactobacillus amylovorus Isolates Derived from Wild Boars and Domestic Pigs

MORAVKOVA, Monika, Iveta KOSTOVOVA, Kateřina KAVANOVÁ, Radko PECHAR, Stanislav STANEK et. al.

Basic information

Original name

Antibiotic Susceptibility, Resistance Gene Determinants and Corresponding Genomic Regions in Lactobacillus amylovorus Isolates Derived from Wild Boars and Domestic Pigs

Authors

MORAVKOVA, Monika (guarantor), Iveta KOSTOVOVA, Kateřina KAVANOVÁ (203 Czech Republic, belonging to the institution), Radko PECHAR, Stanislav STANEK, Ales BRYCHTA, Michal ZEMAN and Tereza KUBASOVA

Edition

Microorganisms, Basel, MDPI, 2023, 2076-2607

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

40301 Veterinary science

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.500 in 2022

RIV identification code

RIV/00216224:14310/23:00130450

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.3390/microorganisms11010103

UT WoS

000927154500001

Keywords in English

antibiotic resistance; tetW; ermB; Lactobacillus amylovorus; domestic pigs; wild boars

Abstract

V originále

Restrictions on the use of antibiotics in pigs lead to the continuous search for new probiotics serving as an alternative to antibiotics. One of the key parameters for probiotic bacteria selection is the absence of horizontally transmissible resistance genes. The aim of our study was to determine antibiotic susceptibility profiles in 28 Lactobacillus amylovorus isolates derived from the digestive tract of wild boars and farm pigs by means of the broth microdilution method and whole genome sequencing (WGS). We revealed genetic resistance determinants and examined sequences flanking resistance genes in these strains. Our findings indicate that L. amylovorus strains from domestic pigs are predominantly resistant to tetracycline, erythromycin and ampicillin. WGS analysis of horizontally transmissible genes revealed only three genetic determinants (tetW, ermB and aadE) of which all tetW and ermB genes were present only in strains derived from domestic pigs. Sequence analysis of coding sequences (CDS) in the neighborhood of the tetW gene revealed the presence of site-specific recombinase (xerC/D), site-specific DNA recombinase (spoIVCA) or DNA-binding transcriptional regulator (xre), usually directly downstream of the tetW gene. In the case of ermB, CDS for omega transcriptional repressor or mobilization protein were detected upstream of the ermB gene.

Citovat

MORAVKOVA, Monika, Iveta KOSTOVOVA, Kateřina KAVANOVÁ, Radko PECHAR, Stanislav STANEK, Ales BRYCHTA, Michal ZEMAN and Tereza KUBASOVA. Antibiotic Susceptibility, Resistance Gene Determinants and Corresponding Genomic Regions in Lactobacillus amylovorus Isolates Derived from Wild Boars and Domestic Pigs. Microorganisms. Basel: MDPI, 2023, vol. 11, No 1, p. 1-17. ISSN 2076-2607. Available from: https://dx.doi.org/10.3390/microorganisms11010103.

@article{2267478,
   author = {Moravkova, Monika and Kostovova, Iveta and Kavanová, Kateřina and Pechar, Radko and Stanek, Stanislav and Brychta, Ales and Zeman, Michal and Kubasova, Tereza},
   article_location = {Basel},
   article_number = {1},
   doi = {http://dx.doi.org/10.3390/microorganisms11010103},
   keywords = {antibiotic resistance; tetW; ermB; Lactobacillus amylovorus; domestic pigs; wild boars},
   language = {eng},
   issn = {2076-2607},
   journal = {Microorganisms},
   title = {Antibiotic Susceptibility, Resistance Gene Determinants and Corresponding Genomic Regions in Lactobacillus amylovorus Isolates Derived from Wild Boars and Domestic Pigs},
   url = {https://www.mdpi.com/2076-2607/11/1/103},
   volume = {11},
   year = {2023}
}

TY  - JOUR
ID  - 2267478
AU  - Moravkova, Monika - Kostovova, Iveta - Kavanová, Kateřina - Pechar, Radko - Stanek, Stanislav - Brychta, Ales - Zeman, Michal - Kubasova, Tereza
PY  - 2023
TI  - Antibiotic Susceptibility, Resistance Gene Determinants and Corresponding Genomic Regions in Lactobacillus amylovorus Isolates Derived from Wild Boars and Domestic Pigs
JF  - Microorganisms
VL  - 11
IS  - 1
SP  - 1-17
EP  - 1-17
PB  - MDPI
SN  - 20762607
KW  - antibiotic resistance
KW  - tetW
KW  - ermB
KW  - Lactobacillus amylovorus
KW  - domestic pigs
KW  - wild boars
UR  - https://www.mdpi.com/2076-2607/11/1/103
N2  - Restrictions on the use of antibiotics in pigs lead to the continuous search for new probiotics serving as an alternative to antibiotics. One of the key parameters for probiotic bacteria selection is the absence of horizontally transmissible resistance genes. The aim of our study was to determine antibiotic susceptibility profiles in 28 Lactobacillus amylovorus isolates derived from the digestive tract of wild boars and farm pigs by means of the broth microdilution method and whole genome sequencing (WGS). We revealed genetic resistance determinants and examined sequences flanking resistance genes in these strains. Our findings indicate that L. amylovorus strains from domestic pigs are predominantly resistant to tetracycline, erythromycin and ampicillin. WGS analysis of horizontally transmissible genes revealed only three genetic determinants (tetW, ermB and aadE) of which all tetW and ermB genes were present only in strains derived from domestic pigs. Sequence analysis of coding sequences (CDS) in the neighborhood of the tetW gene revealed the presence of site-specific recombinase (xerC/D), site-specific DNA recombinase (spoIVCA) or DNA-binding transcriptional regulator (xre), usually directly downstream of the tetW gene. In the case of ermB, CDS for omega transcriptional repressor or mobilization protein were detected upstream of the ermB gene.
ER  -

MORAVKOVA, Monika, Iveta KOSTOVOVA, Kateřina KAVANOVÁ, Radko PECHAR, Stanislav STANEK, Ales BRYCHTA, Michal ZEMAN and Tereza KUBASOVA. Antibiotic Susceptibility, Resistance Gene Determinants and Corresponding Genomic Regions in Lactobacillus amylovorus Isolates Derived from Wild Boars and Domestic Pigs. \textit{Microorganisms}. Basel: MDPI, 2023, vol.~11, No~1, p.~1-17. ISSN~2076-2607. Available from: https://dx.doi.org/10.3390/microorganisms11010103.

Detailed Information on Publication Record