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@article{2267698, author = {Manová, Anna and Hýžová, Blanka and Canora, Diana Darriba and Antolin, Ana Castrillo and Dufková, Kristýna}, article_location = {Brno}, article_number = {2}, doi = {http://dx.doi.org/10.5817/CPR2022-2-20}, keywords = {lichen photobionts; algal biotechnology; Kautsky kinetics}, language = {eng}, issn = {1805-0689}, journal = {Czech polar reports}, title = {Vitality and growth rate of agar plate-cultivated Antarctic microautotrophs: Analysis of PSII functioning by chlorophyll fluorescence parameters}, url = {https://journals.muni.cz/CPR/article/view/33535}, volume = {12}, year = {2022} }
TY - JOUR ID - 2267698 AU - Manová, Anna - Hýžová, Blanka - Canora, Diana Darriba - Antolin, Ana Castrillo - Dufková, Kristýna PY - 2022 TI - Vitality and growth rate of agar plate-cultivated Antarctic microautotrophs: Analysis of PSII functioning by chlorophyll fluorescence parameters JF - Czech polar reports VL - 12 IS - 2 SP - 269-279 EP - 269-279 PB - Masaryk University SN - 18050689 KW - lichen photobionts KW - algal biotechnology KW - Kautsky kinetics UR - https://journals.muni.cz/CPR/article/view/33535 N2 - In our study, we focused on the growth of three different microautotrophs isolated from Antarctic lichens (Placopsis contortuplicata, Solorina spongiosa) and cryptoendolithic algal vegetation. The isolates were purified and inoculated on agar plates, Bold ' s Basal Medium (BBM). The growth of the cultures and the markers of physiological (photo-synthetic) activity were monitored by chlorophyll fluorescence in 1 week intervals for 3 months after inoculation. For the assessment of photosynthetic activity, the meth-od of slow Kautsky kinetics supplemented with saturation pulses was applied. Four chlorophyll fluorescence parameters calculated: (1) maximum quantum yield of PSII (FV/FM), (2) effective quantum yield of photosynthetic processes in PSII (phi PSII), (3) non -photochemical quenching of chlorophyll fluorescence, and (4) background chlorophyll fluorescence ratio (F0/F0 '). Troughout the cultivation period, the maximum quantum yield of PSII (FV/FM) showed high values in all three autotrophs with only slight increase in the first part of the cultivation period, followed by slight decrease in the second part. The phi PSII values showed a rapid decline within the first 4 weeks of cultivation followed by more or less constant values in the isolates from P. contortuplicata and cryptoen-dolithic alga. Contrastingly, time course of phi PSII rather showed an increase followed by a decrease in S. spongiosa isolate. NPQ (related to the activation of protective mecha-nisms) increased in the second part of cultivation period, the rate of increase and maxi-mum values were species-specific. The species-specific differences in chlorophyll fluo-rescence parameters are discussed as well as their potential for evaluation of photo-synthetic performance of in vitro cultivated algal/cyanobacterial cultures on agar plates. ER -
MANOVÁ, Anna, Blanka HÝŽOVÁ, Diana Darriba CANORA, Ana Castrillo ANTOLIN and Kristýna DUFKOVÁ. Vitality and growth rate of agar plate-cultivated Antarctic microautotrophs: Analysis of PSII functioning by chlorophyll fluorescence parameters. \textit{Czech polar reports}. Brno: Masaryk University, 2022, vol.~12, No~2, p.~269-279. ISSN~1805-0689. Available from: https://dx.doi.org/10.5817/CPR2022-2-20.
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