Detailed Information on Publication Record
2022
Vitality and growth rate of agar plate-cultivated Antarctic microautotrophs: Analysis of PSII functioning by chlorophyll fluorescence parameters
MANOVÁ, Anna, Blanka HÝŽOVÁ, Diana Darriba CANORA, Ana Castrillo ANTOLIN, Kristýna DUFKOVÁ et. al.Basic information
Original name
Vitality and growth rate of agar plate-cultivated Antarctic microautotrophs: Analysis of PSII functioning by chlorophyll fluorescence parameters
Authors
MANOVÁ, Anna (203 Czech Republic, belonging to the institution), Blanka HÝŽOVÁ (203 Czech Republic, belonging to the institution), Diana Darriba CANORA (724 Spain), Ana Castrillo ANTOLIN (724 Spain) and Kristýna DUFKOVÁ (203 Czech Republic, guarantor, belonging to the institution)
Edition
Czech polar reports, Brno, Masaryk University, 2022, 1805-0689
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10611 Plant sciences, botany
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 1.000
RIV identification code
RIV/00216224:14310/22:00128888
Organization unit
Faculty of Science
UT WoS
000935360100010
Keywords in English
lichen photobionts; algal biotechnology; Kautsky kinetics
Tags
International impact, Reviewed
Změněno: 11/7/2023 10:12, Mgr. Marie Šípková, DiS.
Abstract
V originále
In our study, we focused on the growth of three different microautotrophs isolated from Antarctic lichens (Placopsis contortuplicata, Solorina spongiosa) and cryptoendolithic algal vegetation. The isolates were purified and inoculated on agar plates, Bold ' s Basal Medium (BBM). The growth of the cultures and the markers of physiological (photo-synthetic) activity were monitored by chlorophyll fluorescence in 1 week intervals for 3 months after inoculation. For the assessment of photosynthetic activity, the meth-od of slow Kautsky kinetics supplemented with saturation pulses was applied. Four chlorophyll fluorescence parameters calculated: (1) maximum quantum yield of PSII (FV/FM), (2) effective quantum yield of photosynthetic processes in PSII (phi PSII), (3) non -photochemical quenching of chlorophyll fluorescence, and (4) background chlorophyll fluorescence ratio (F0/F0 '). Troughout the cultivation period, the maximum quantum yield of PSII (FV/FM) showed high values in all three autotrophs with only slight increase in the first part of the cultivation period, followed by slight decrease in the second part. The phi PSII values showed a rapid decline within the first 4 weeks of cultivation followed by more or less constant values in the isolates from P. contortuplicata and cryptoen-dolithic alga. Contrastingly, time course of phi PSII rather showed an increase followed by a decrease in S. spongiosa isolate. NPQ (related to the activation of protective mecha-nisms) increased in the second part of cultivation period, the rate of increase and maxi-mum values were species-specific. The species-specific differences in chlorophyll fluo-rescence parameters are discussed as well as their potential for evaluation of photo-synthetic performance of in vitro cultivated algal/cyanobacterial cultures on agar plates.
Links
| EF16_013/0001708, research and development project |
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