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@inproceedings{2268703, author = {Trnková, Libuše and Třísková, Iveta and Farka, Zdeněk}, address = {Ústí nad Labem}, booktitle = {41. Moderní elektrochemické metody : Sborník přednášek}, editor = {Navrátil, Tomáš; Fojta, Miroslav; Schwarzová}, keywords = {Insulin; Tyrosine; Screen-printed graphite electrode; Pencil graphite electrodes; Voltammetry; Circular dichroism; Denaturation; BeStSel}, howpublished = {tištěná verze "print"}, language = {eng}, location = {Ústí nad Labem}, isbn = {978-80-905221-9-0}, pages = {200-203}, publisher = {Best Servis}, title = {Electrochemical Analysis of Insulin on Graphite Electrodes}, url = {https://www.bestservis.eu/images/file/Sbornik_metody22.pdf}, year = {2022} }
TY - JOUR ID - 2268703 AU - Trnková, Libuše - Třísková, Iveta - Farka, Zdeněk PY - 2022 TI - Electrochemical Analysis of Insulin on Graphite Electrodes PB - Best Servis CY - Ústí nad Labem SN - 9788090522190 KW - Insulin KW - Tyrosine KW - Screen-printed graphite electrode KW - Pencil graphite electrodes KW - Voltammetry KW - Circular dichroism KW - Denaturation KW - BeStSel UR - https://www.bestservis.eu/images/file/Sbornik_metody22.pdf N2 - Electrochemical study of insulin on non-modified and nanoparticle-modified graphite electrodes was performed. The insulin oxidation signal, for which tyrosine is responsible, has been investigated not only in terms of insulin forms (native, denatured, dimeric, monomeric) and adjustable solution parameters (pH, concentration, ionic strength, and buffer) but also in terms of the modification of electrode surface. It was found that all the above-mentioned parameters have effect on insulin oxidation, but the greatest changes in voltammetric signals were caused by the nano-modification of electrode surfaces and the structure of insulin. The information about the secondary structure of insulin in solutions was obtained by measuring circular dichroism spectra and their subsequent evaluation using a novel method for secondary structure determination of proteins BeStSel (Beta Structure Selection). ER -
TRNKOVÁ, Libuše, Iveta TŘÍSKOVÁ and Zdeněk FARKA. Electrochemical Analysis of Insulin on Graphite Electrodes. In Navrátil, Tomáš; Fojta, Miroslav; Schwarzová. \textit{41. Moderní elektrochemické metody : Sborník přednášek}. Ústí nad Labem: Best Servis, 2022, p.~200-203. ISBN~978-80-905221-9-0.
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