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@article{2269877,
author = {Kryl, Martin and Sedláček, Vojtěch and Kučera, Igor},
article_location = {Basel},
article_number = {4},
doi = {http://dx.doi.org/10.3390/ijms24043732},
keywords = {FMN; NADH; dioxygen reduction; Paracoccus denitrificans},
language = {eng},
issn = {1422-0067},
journal = {International Journal of Molecular Sciences},
title = {Structural Insight into Catalysis by the Flavin-Dependent NADH Oxidase (Pden_5119) of Paracoccus denitrificans},
url = {https://www.mdpi.com/1422-0067/24/4/3732},
volume = {24},
year = {2023}
}
TY - JOUR
ID - 2269877
AU - Kryl, Martin - Sedláček, Vojtěch - Kučera, Igor
PY - 2023
TI - Structural Insight into Catalysis by the Flavin-Dependent NADH Oxidase (Pden_5119) of Paracoccus denitrificans
JF - International Journal of Molecular Sciences
VL - 24
IS - 4
SP - 1-17
EP - 1-17
PB - Multidisciplinary Digital Publishing Institute
SN - 14220067
KW - FMN
KW - NADH
KW - dioxygen reduction
KW - Paracoccus denitrificans
UR - https://www.mdpi.com/1422-0067/24/4/3732
N2 - The Pden_5119 protein oxidizes NADH with oxygen under mediation by the bound flavin mononucleotide (FMN) and may be involved in the maintenance of the cellular redox pool. In biochemical characterization, the curve of the pH-rate dependence was bell-shaped with pK(a1) = 6.6 and pK(a2) = 9.2 at 2 mu M FMN while it contained only a descending limb pK(a) of 9.7 at 50 mu M FMN. The enzyme was found to undergo inactivation by reagents reactive with histidine, lysine, tyrosine, and arginine. In the first three cases, FMN exerted a protective effect against the inactivation. X-ray structural analysis coupled with site-directed mutagenesis identified three amino acid residues important to the catalysis. Structural and kinetic data suggest that His-117 plays a role in the binding and positioning of the isoalloxazine ring of FMN, Lys-82 fixes the nicotinamide ring of NADH to support the proS-hydride transfer, and Arg-116 with its positive charge promotes the reaction between dioxygen and reduced flavin.
ER -
KRYL, Martin, Vojtěch SEDLÁČEK and Igor KUČERA. Structural Insight into Catalysis by the Flavin-Dependent NADH Oxidase (Pden\_{}5119) of Paracoccus denitrificans. \textit{International Journal of Molecular Sciences}. Basel: Multidisciplinary Digital Publishing Institute, 2023, vol.~24, No~4, p.~1-17. ISSN~1422-0067. Available from: https://dx.doi.org/10.3390/ijms24043732.
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