Eudragit® RL coated pellets with immobilized
butyrylcholinesterase for rapid detection of cholinesterase
inhibitors in water

a 2023

Eudragit® RL coated pellets with immobilized butyrylcholinesterase for rapid detection of cholinesterase inhibitors in water

ZEMAN, Jiří, David VETCHÝ, Sylvie PAVLOKOVÁ, Aleš FRANC, Vladimír PITSCHMANN et. al.

Basic information

Original name

Eudragit® RL coated pellets with immobilized butyrylcholinesterase for rapid detection of cholinesterase inhibitors in water

Authors

ZEMAN, Jiří (203 Czech Republic, guarantor, belonging to the institution), David VETCHÝ (203 Czech Republic, belonging to the institution), Sylvie PAVLOKOVÁ (203 Czech Republic, belonging to the institution), Aleš FRANC (203 Czech Republic, belonging to the institution) and Vladimír PITSCHMANN (203 Czech Republic)

Edition

4th European Conference on Pharmaceutics, 2023

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

30104 Pharmacology and pharmacy

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Webová stránka konference

RIV identification code

RIV/00216224:14160/23:00134605

Organization unit

Faculty of Pharmacy

Keywords in English

detection tube; nerve agent; Neusilin; cholinesterase; color transition; stability

Abstract

V originále

Nerve agents (soman, sarin, tabun, VX), organophosphate (parathion, malathion) and carbamate (aldicarb) pesticides are commonly referred to as cholinesterase inhibitors (ChI), which are substances with very high toxicity. A fast, cheap, and sensitive sensor is needed to detect ChI as quickly as possible in order to carry out the appropriate treatment for those affected. Detection tubes (DTs) that use pellets with immobilized cholinesterase for colorimetric detection of the ChI presence, are just the type of detector that meets the above-mentioned requirements. Moreover, enzyme-based biosensors such as DTs have a detection limit between 0.005–0.01 mg∙m−3, which is the lowest in comparison with more expensive and unportable detectors using GC-MS, FTIR or IMS. DTs are based on fast enzymatic Ellman reaction. However, it suffers from an indistinct color transition from white to yellow in the absence of ChI. In this study, an innovative carrier in the form of coated matrix pellets was prepared utilizing the advantages of the previous experiment. The pellet cores contained the unique sorbent – Neusilin® US2 and the semipermeable coating consisted mainly of Eudragit® RL. The main aim of the experiment was to evaluate reaction rate, sensitivity, and color transition intensity of prepared samples in comparison with previous types of carriers.

Links

VI20192022172, research and development project

Name: Detekční trubičky druhé generace pro kontinuální a opakované monitorování nervově paralytických a jiných toxických látek

Investor: Ministry of the Interior of the CR

Citovat

ZEMAN, Jiří, David VETCHÝ, Sylvie PAVLOKOVÁ, Aleš FRANC and Vladimír PITSCHMANN. Eudragit® RL coated pellets with immobilized butyrylcholinesterase for rapid detection of cholinesterase inhibitors in water. In 4th European Conference on Pharmaceutics. 2023.

@proceedings{2270422,
   author = {Zeman, Jiří and Vetchý, David and Pavloková, Sylvie and Franc, Aleš and Pitschmann, Vladimír},
   booktitle = {4th European Conference on Pharmaceutics},
   keywords = {detection tube; nerve agent; Neusilin; cholinesterase; color transition; stability},
   language = {eng},
   title = {Eudragit® RL coated pellets with immobilized butyrylcholinesterase for rapid detection of cholinesterase inhibitors in water},
   url = {https://www.europeanmeeting.org/home/},
   year = {2023}
}

TY  - CONF
ID  - 2270422
AU  - Zeman, Jiří - Vetchý, David - Pavloková, Sylvie - Franc, Aleš - Pitschmann, Vladimír
PY  - 2023
TI  - Eudragit® RL coated pellets with immobilized butyrylcholinesterase for rapid detection of cholinesterase inhibitors in water
KW  - detection tube
KW  - nerve agent
KW  - Neusilin
KW  - cholinesterase
KW  - color transition
KW  - stability
UR  - https://www.europeanmeeting.org/home/
N2  - Nerve agents (soman, sarin, tabun, VX), organophosphate (parathion, malathion) and carbamate (aldicarb) pesticides are commonly referred to as cholinesterase inhibitors (ChI), which are substances with very high toxicity. A fast, cheap, and sensitive sensor is needed to detect ChI as quickly as possible in order to carry out the appropriate treatment for those affected. Detection tubes (DTs) that use pellets with immobilized cholinesterase for colorimetric detection of the ChI presence, are just the type of detector that meets the above-mentioned requirements. Moreover, enzyme-based biosensors such as DTs have a detection limit between 0.005–0.01 mg∙m−3, which is the lowest in comparison with more expensive and unportable detectors using GC-MS, FTIR or IMS. DTs are based on fast enzymatic Ellman reaction. However, it suffers from an indistinct color transition from white to yellow in the absence of ChI. In this study, an innovative carrier in the form of coated matrix pellets was prepared utilizing the advantages of the previous experiment. The pellet cores contained the unique sorbent – Neusilin® US2 and the semipermeable coating consisted mainly of Eudragit® RL. The main aim of the experiment was to evaluate reaction rate, sensitivity, and color transition intensity of prepared samples in comparison with previous types of carriers.
ER  -

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