Use of all-male triploid population of zebrafish (Danio rerio)
for evaluation of 17 alpha-ethinylestradiol effects

J 2022

Use of all-male triploid population of zebrafish (Danio rerio) for evaluation of 17 alpha-ethinylestradiol effects

WEISEROVA, Zuzana, Viola ZENTRICHOVA, Denisa MEDKOVA, Jana BLAHOVA, Aleš FRANC et. al.

Basic information

Original name

Use of all-male triploid population of zebrafish (Danio rerio) for evaluation of 17 alpha-ethinylestradiol effects

Authors

WEISEROVA, Zuzana (guarantor), Viola ZENTRICHOVA, Denisa MEDKOVA, Jana BLAHOVA, Aleš FRANC (203 Czech Republic, belonging to the institution), Michaela GARAJOVA, Jan MARES, Eva POSTULKOVA, Roman FRANEK, Martin PSENICKA, Petr MARSALEK and Zdenka SVOBODOVA

Edition

Neuroendocrinology letters, MUNSBACH, MAGHIRA & MAAS PUBLICATIONS, 2022, 0172-780X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30104 Pharmacology and pharmacy

Country of publisher

Luxembourg

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 0.700

RIV identification code

RIV/00216224:14160/22:00128945

Organization unit

Faculty of Pharmacy

UT WoS

000942362300005

Keywords in English

EE2; feed contamination; oral intake; vitellogenin; xenoestrogen testing

Abstract

V originále

OBJECTIVES: We tested the toxicity of ethinylestradiol, a semisynthetic estrogen used in oral contraceptives, on all-male triploid zebrafish using commercial feeds and three different doses concentrations. We aimed to determine whether ethinylestradiol peroral administration resulted in vitellogenin production and whether all-male triploid zebrafish could serve as a model species for xenoestrogen testing. METHODS: The actual concentrations of 17 alpha-ethinylestradiol were 0.0035 (low); 0.0315 (medium) and 0.365 (high) mu g/g. Positive control represented commercial feeds containing 0.0465 mu g/g of beta-estradiol. The experiment lasted 8 weeks. RESULTS: Our results indicate that 17 alpha-ethinylestradiol consumption does induce vitellogenin production in triploid zebrafish. CONCLUSIONS: The simple presence of vitellogenin is a definite symptom indicative of the potential for such changes due to the action of estrogenic substances. As such, this experiment has shown that the use of all-male triploid zebrafish populations, rather than the mixed-sex populations of other species previously used, could serve as a suitable alternative model population for controlled testing of the effects of xenoestrogens on fish.

Citovat

WEISEROVA, Zuzana, Viola ZENTRICHOVA, Denisa MEDKOVA, Jana BLAHOVA, Aleš FRANC, Michaela GARAJOVA, Jan MARES, Eva POSTULKOVA, Roman FRANEK, Martin PSENICKA, Petr MARSALEK and Zdenka SVOBODOVA. Use of all-male triploid population of zebrafish (Danio rerio) for evaluation of 17 alpha-ethinylestradiol effects. Neuroendocrinology letters. MUNSBACH: MAGHIRA & MAAS PUBLICATIONS, 2022, vol. 43, No 5, p. 281-290. ISSN 0172-780X.

@article{2270797,
   author = {Weiserova, Zuzana and Zentrichova, Viola and Medkova, Denisa and Blahova, Jana and Franc, Aleš and Garajova, Michaela and Mares, Jan and Postulkova, Eva and Franek, Roman and Psenicka, Martin and Marsalek, Petr and Svobodova, Zdenka},
   article_location = {MUNSBACH},
   article_number = {5},
   keywords = {EE2; feed contamination; oral intake; vitellogenin; xenoestrogen testing},
   language = {eng},
   issn = {0172-780X},
   journal = {Neuroendocrinology letters},
   title = {Use of all-male triploid population of zebrafish (Danio rerio) for evaluation of 17 alpha-ethinylestradiol effects},
   url = {https://www.nel.edu/userfiles/articlesnew/1674499217_43_5_weiserova_281-pdf.pdf},
   volume = {43},
   year = {2022}
}

TY  - JOUR
ID  - 2270797
AU  - Weiserova, Zuzana - Zentrichova, Viola - Medkova, Denisa - Blahova, Jana - Franc, Aleš - Garajova, Michaela - Mares, Jan - Postulkova, Eva - Franek, Roman - Psenicka, Martin - Marsalek, Petr - Svobodova, Zdenka
PY  - 2022
TI  - Use of all-male triploid population of zebrafish (Danio rerio) for evaluation of 17 alpha-ethinylestradiol effects
JF  - Neuroendocrinology letters
VL  - 43
IS  - 5
SP  - 281-290
EP  - 281-290
PB  - MAGHIRA & MAAS PUBLICATIONS
SN  - 0172780X
KW  - EE2
KW  - feed contamination
KW  - oral intake
KW  - vitellogenin
KW  - xenoestrogen testing
UR  - https://www.nel.edu/userfiles/articlesnew/1674499217_43_5_weiserova_281-pdf.pdf
N2  - OBJECTIVES: We tested the toxicity of ethinylestradiol, a semisynthetic estrogen used in oral contraceptives, on all-male triploid zebrafish using commercial feeds and three different doses concentrations. We aimed to determine whether ethinylestradiol peroral administration resulted in vitellogenin production and whether all-male triploid zebrafish could serve as a model species for xenoestrogen testing. METHODS: The actual concentrations of 17 alpha-ethinylestradiol were 0.0035 (low); 0.0315 (medium) and 0.365 (high) mu g/g. Positive control represented commercial feeds containing 0.0465 mu g/g of beta-estradiol. The experiment lasted 8 weeks. RESULTS: Our results indicate that 17 alpha-ethinylestradiol consumption does induce vitellogenin production in triploid zebrafish. CONCLUSIONS: The simple presence of vitellogenin is a definite symptom indicative of the potential for such changes due to the action of estrogenic substances. As such, this experiment has shown that the use of all-male triploid zebrafish populations, rather than the mixed-sex populations of other species previously used, could serve as a suitable alternative model population for controlled testing of the effects of xenoestrogens on fish.
ER  -

WEISEROVA, Zuzana, Viola ZENTRICHOVA, Denisa MEDKOVA, Jana BLAHOVA, Aleš FRANC, Michaela GARAJOVA, Jan MARES, Eva POSTULKOVA, Roman FRANEK, Martin PSENICKA, Petr MARSALEK and Zdenka SVOBODOVA. Use of all-male triploid population of zebrafish (Danio rerio) for evaluation of 17 alpha-ethinylestradiol effects. \textit{Neuroendocrinology letters}. MUNSBACH: MAGHIRA \&{} MAAS PUBLICATIONS, 2022, vol.~43, No~5, p.~281-290. ISSN~0172-780X.

Detailed Information on Publication Record