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@article{2271653,
author = {Mahrík, Lenka and Štefanovie, Barbora and Maresova, Anna and Princova, Jarmila and Kolesár, Peter and Lelkes, Edit and Faux, Celline and Helmlinger, Dominique and Prevorovsky, Martin and Paleček, Jan},
article_location = {LONDON},
article_number = {1},
doi = {http://dx.doi.org/10.1186/s13072-023-00480-z},
keywords = {Genetic and protein-protein interactions; SMC5/6 complex; Nse3 KITE; SAGA histone acetyltransferase module; Gcn5; Ada2; Chromatin accessibility; DNA repair; rDNA; Gene regions},
language = {eng},
issn = {1756-8935},
journal = {EPIGENETICS & CHROMATIN},
title = {The SAGA histone acetyltransferase module targets SMC5/6 to specific genes},
url = {https://epigeneticsandchromatin.biomedcentral.com/articles/10.1186/s13072-023-00480-z},
volume = {16},
year = {2023}
}
TY - JOUR
ID - 2271653
AU - Mahrík, Lenka - Štefanovie, Barbora - Maresova, Anna - Princova, Jarmila - Kolesár, Peter - Lelkes, Edit - Faux, Celline - Helmlinger, Dominique - Prevorovsky, Martin - Paleček, Jan
PY - 2023
TI - The SAGA histone acetyltransferase module targets SMC5/6 to specific genes
JF - EPIGENETICS & CHROMATIN
VL - 16
IS - 1
SP - 1-16
EP - 1-16
PB - BMC
SN - 17568935
KW - Genetic and protein-protein interactions
KW - SMC5/6 complex
KW - Nse3 KITE
KW - SAGA histone acetyltransferase module
KW - Gcn5
KW - Ada2
KW - Chromatin accessibility
KW - DNA repair
KW - rDNA
KW - Gene regions
UR - https://epigeneticsandchromatin.biomedcentral.com/articles/10.1186/s13072-023-00480-z
N2 - Structural Maintenance of Chromosomes (SMC) complexes are molecular machines driving chromatin organization at higher levels. In eukaryotes, three SMC complexes (cohesin, condensin and SMC5/6) play key roles in cohesion, condensation, replication, transcription and DNA repair. Their physical binding to DNA requires accessible chromatin. We performed a genetic screen in fission yeast to identify novel factors required for SMC5/6 binding to DNA. We identified 79 genes of which histone acetyltransferases (HATs) were the most represented. Genetic and phenotypic analyses suggested a particularly strong functional relationship between the SMC5/6 and SAGA complexes. Furthermore, several SMC5/6 subunits physically interacted with SAGA HAT module components Gcn5 and Ada2. As Gcn5-dependent acetylation facilitates the accessibility of chromatin to DNA-repair proteins, we first analysed the formation of DNA-damage-induced SMC5/6 foci in the Delta gcn5 mutant. The SMC5/6 foci formed normally in Delta gcn5, suggesting SAGA-independent SMC5/6 localization to DNA-damaged sites. Next, we used Nse4-FLAG chromatin-immunoprecipitation (ChIP-seq) analysis in unchallenged cells to assess SMC5/6 distribution. A significant portion of SMC5/6 accumulated within gene regions in wild-type cells, which was reduced in Delta gcn5 and Delta ada2 mutants. The drop in SMC5/6 levels was also observed in gcn5-E191Q acetyltransferase-dead mutant. Our data show genetic and physical interactions between SMC5/6 and SAGA complexes. The ChIP-seq analysis suggests that SAGA HAT module targets SMC5/6 to specific gene regions and facilitates their accessibility for SMC5/6 loading.
ER -
MAHRÍK, Lenka, Barbora ŠTEFANOVIE, Anna MARESOVA, Jarmila PRINCOVA, Peter KOLESÁR, Edit LELKES, Celline FAUX, Dominique HELMLINGER, Martin PREVOROVSKY and Jan PALEČEK. The SAGA histone acetyltransferase module targets SMC5/6 to specific genes. \textit{EPIGENETICS \&{} CHROMATIN}. LONDON: BMC, 2023, vol.~16, No~1, p.~1-16. ISSN~1756-8935. Available from: https://dx.doi.org/10.1186/s13072-023-00480-z.
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