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@article{2271920,
author = {Nekola, Jeffrey Clark and Nováková, Markéta and Horsák, Michal and Adema, Coen M.},
article_number = {3},
doi = {http://dx.doi.org/10.1007/s13127-022-00587-3},
keywords = {PCR; nDNA; Intron; Phylogenetic marker; Gastropods},
language = {eng},
issn = {1439-6092},
journal = {Organisms Diversity and Evolution},
title = {ELAV Intron 8: a single-copy sequence marker for shallow to deep phylogeny in Eupulmonata Hasprunar & Huber, 1990 and Hygrophila Ferussac, 1822 (Gastropoda: Mollusca)},
url = {https://doi.org/10.1007/s13127-022-00587-3},
volume = {23},
year = {2023}
}
TY - JOUR
ID - 2271920
AU - Nekola, Jeffrey Clark - Nováková, Markéta - Horsák, Michal - Adema, Coen M.
PY - 2023
TI - ELAV Intron 8: a single-copy sequence marker for shallow to deep phylogeny in Eupulmonata Hasprunar & Huber, 1990 and Hygrophila Ferussac, 1822 (Gastropoda: Mollusca)
JF - Organisms Diversity and Evolution
VL - 23
IS - 3
SP - 621-629
EP - 621-629
PB - Springer
SN - 14396092
KW - PCR
KW - nDNA
KW - Intron
KW - Phylogenetic marker
KW - Gastropods
UR - https://doi.org/10.1007/s13127-022-00587-3
N2 - Progress in eupulmonate gastropod taxonomy is limited by absence of single- or low-copy nuclear DNA markers that provide species-scale insights. We detail here the single-copy intron 8 of the embryonic lethality and abnormal visual system gene (ELAVI8). High sequence conservation within flanking exons 8 and 9 allowed design of non-redundant primers that enable PCR amplification across a broad phylogenetic extent. Across the Eupulmonata and Hygrophila ELAVI8 product ranged from 602 to 802 base pairs in length. A multiple sequence alignment across 62 taxa, representing 36 genera and 12 major clades, showed 1296 sites with 865 variable positions. Typically, 2-10 base pair differences were noted between closely related species within a genus, with 74-200 bases differing between major infra-order clades. ELAVI8 maximum-likelihood trees showed essentially identical topologies at high levels of support compared to those created from concatenated ITS1 + 2 (within a genus) or 28S (across Eupulmonata and Hygrophila). ELAVI8 is superior to these two phylogenetic marker regions, however, because it (1) resolves phylogenetic pattern from species- to sub-cohort scales in a single amplicon, (2) was better able to resolve family- and deeper-clades at high support, (3) allows for outgroup rooting between genera, and (4) possesses no intercopy haplotype variability.
ER -
NEKOLA, Jeffrey Clark, Markéta NOVÁKOVÁ, Michal HORSÁK and Coen M. ADEMA. ELAV Intron 8: a single-copy sequence marker for shallow to deep phylogeny in Eupulmonata Hasprunar \&{}amp; Huber, 1990 and Hygrophila Ferussac, 1822 (Gastropoda: Mollusca). \textit{Organisms Diversity and Evolution}. Springer, 2023, vol.~23, No~3, p.~621-629. ISSN~1439-6092. Available from: https://dx.doi.org/10.1007/s13127-022-00587-3.
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