Synthetic long reads reconstruction using molecular barcoding

DAVYDOV, Alexey Nikolayevich. Synthetic long reads reconstruction using molecular barcoding. 2022.

Basic information

• Original name: Synthetic long reads reconstruction using molecular barcoding
• Name in Czech: Rekonstrukce syntetických dlouhých sekvencí za použití molekulárního barkodování
• Authors: DAVYDOV, Alexey Nikolayevich (643 Russian Federation, guarantor, belonging to the institution).
• Edition: 2022.

Other information

• Original language: English
• Type of outcome: Research and development projects
• Field of Study: 10608 Biochemistry and molecular biology
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14740/22:00129621
• Organization unit: Central European Institute of Technology
• Keywords (in Czech): molekulární čárové kódy; unikátní molekulární identifikátory;oligonukleotidy;PCR;příprava knihoven
• Keywords in English: molecular barcoding;unique molecular identifiers;oligonucleotides;polymerase-based reaction;library preparation
• Tags: rivok

Abstract

The project aims to implement our newly developed method for the unique molecular identifiers (UMIs) introduction into oligonucleotides. This method can be used for RNA/DNA next generation sequencing (NGS) libraries preparation. The structure of the generated sequencing libraries with the introduced UMIs allows for a downstream long-reads reconstruction and PCR error correction resulting in accurately quantified ‚phased‘ genomic sequences or full-length transcripts. The potential range of the technology applications is very broad, and includes various fields, such as medical genomics, pharmacogenomics, molecular diagnostics, cancer genomics, and others. In this project, we focused on applying the method for the analysis 16S RNA sequencing (metagenomics) and immunogenomics analysis (TCR and BCR repertoires).

Links

• TP01010039, research and development project: Name: Posílení systému komercializace výsledků VaV na Masarykově univerzitě (Acronym: POSTKOM)

Investor: Technology Agency of the Czech Republic, Subprogram 1