Safety assessment and redox status in rats after chronic
exposure to cannabidiol and cannabigerol

J 2023

Safety assessment and redox status in rats after chronic exposure to cannabidiol and cannabigerol

HOLCOVÁ POLANSKÁ, Hana, Kateřina PETRLÁKOVÁ, Barbora PAPOUSKOVA, Michal HENDRYCH, Amir SAMADIAN et. al.

Basic information

Original name

Safety assessment and redox status in rats after chronic exposure to cannabidiol and cannabigerol

Authors

HOLCOVÁ POLANSKÁ, Hana (203 Czech Republic, belonging to the institution), Kateřina PETRLÁKOVÁ (203 Czech Republic, belonging to the institution), Barbora PAPOUSKOVA (203 Czech Republic), Michal HENDRYCH (203 Czech Republic, belonging to the institution), Amir SAMADIAN (364 Islamic Republic of Iran, belonging to the institution), Jan STORCH (203 Czech Republic), Petr BABULA (203 Czech Republic, belonging to the institution), Michal MASAŘÍK (203 Czech Republic, belonging to the institution) and Jan VACEK (203 Czech Republic, guarantor)

Edition

Toxicology, CLARE, ELSEVIER IRELAND LTD, 2023, 0300-483X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30108 Toxicology

Country of publisher

Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.500 in 2022

RIV identification code

RIV/00216224:14110/23:00130557

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.tox.2023.153460

UT WoS

000962570900001

Keywords in English

Phytocannabinoid; in vivo experiment; Antioxidant; CBD protective properties; CBG toxicity

Abstract

V originále

Cannabidiol (CBD) and cannabigerol (CBG) are the two main non-psychotropic phytocannabinoids with high application potential in drug development. Both substances are redox-active and are intensively investigated for their cytoprotective and antioxidant action in vitro. In this study, we focused on an in vivo safety evaluation and the effect of CBD and CBG on the redox status in rats in a 90-d experiment. The substances were administered orogastrically in a dose of 0.66 mg synthetic CBD or 0.66 mg/1.33 mg CBG/kg/day. CBD produced no changes in the red or white blood count or biochemical blood parameters in comparison to the control. No deviations in the morphology or histology of the gastrointestinal tract and liver were observed. After 90 d of CBD exposure, a significant improvement in redox status was found in the blood plasma and liver. The concentration of malondialdehyde and carbonylated proteins was reduced compared to the control. In contrast to CBD, total oxidative stress was significantly increased and this was accompanied by an elevated level of malondialdehyde and carbonylated proteins in CBG-treated animals. Hepatotoxic (regressive changes) manifestations, disruption in white cell count, and alterations in the ALT activity, level of creatinine and ionized calcium were also found in CBG-treated animals. Based on liquid chromatography-mass spectrometry analysis, CBD/CBG accumulated in rat tissues (in the liver, brain, muscle, heart, kidney and skin) at a low ng level per gram. Both CBD and CBG molecular structures include a resorcinol moiety. In CBG, there is an extra dimethyloctadienyl structural pattern, which is most likely responsible for the disruption to the redox status and hepatic environment. The results are valuable to further investigation of the effects of CBD on redox status and should contribute towards opening up critical discussion on the applicability of other non-psychotropic cannabinoids.

Citovat

HOLCOVÁ POLANSKÁ, Hana, Kateřina PETRLÁKOVÁ, Barbora PAPOUSKOVA, Michal HENDRYCH, Amir SAMADIAN, Jan STORCH, Petr BABULA, Michal MASAŘÍK and Jan VACEK. Safety assessment and redox status in rats after chronic exposure to cannabidiol and cannabigerol. Toxicology. CLARE: ELSEVIER IRELAND LTD, 2023, vol. 488, April 2023, p. 1-10. ISSN 0300-483X. Available from: https://dx.doi.org/10.1016/j.tox.2023.153460.

@article{2273648,
   author = {Holcová Polanská, Hana and Petrláková, Kateřina and Papouskova, Barbora and Hendrych, Michal and Samadian, Amir and Storch, Jan and Babula, Petr and Masařík, Michal and Vacek, Jan},
   article_location = {CLARE},
   article_number = {April 2023},
   doi = {http://dx.doi.org/10.1016/j.tox.2023.153460},
   keywords = {Phytocannabinoid; in vivo experiment; Antioxidant; CBD protective properties; CBG toxicity},
   language = {eng},
   issn = {0300-483X},
   journal = {Toxicology},
   title = {Safety assessment and redox status in rats after chronic exposure to cannabidiol and cannabigerol},
   url = {https://www.sciencedirect.com/science/article/pii/S0300483X2300046X?via%3Dihub},
   volume = {488},
   year = {2023}
}

TY  - JOUR
ID  - 2273648
AU  - Holcová Polanská, Hana - Petrláková, Kateřina - Papouskova, Barbora - Hendrych, Michal - Samadian, Amir - Storch, Jan - Babula, Petr - Masařík, Michal - Vacek, Jan
PY  - 2023
TI  - Safety assessment and redox status in rats after chronic exposure to cannabidiol and cannabigerol
JF  - Toxicology
VL  - 488
IS  - April 2023
SP  - 1-10
EP  - 1-10
PB  - ELSEVIER IRELAND LTD
SN  - 0300483X
KW  - Phytocannabinoid
KW  - in vivo experiment
KW  - Antioxidant
KW  - CBD protective properties
KW  - CBG toxicity
UR  - https://www.sciencedirect.com/science/article/pii/S0300483X2300046X?via%3Dihub
N2  - Cannabidiol (CBD) and cannabigerol (CBG) are the two main non-psychotropic phytocannabinoids with high application potential in drug development. Both substances are redox-active and are intensively investigated for their cytoprotective and antioxidant action in vitro. In this study, we focused on an in vivo safety evaluation and the effect of CBD and CBG on the redox status in rats in a 90-d experiment. The substances were administered orogastrically in a dose of 0.66 mg synthetic CBD or 0.66 mg/1.33 mg CBG/kg/day. CBD produced no changes in the red or white blood count or biochemical blood parameters in comparison to the control. No deviations in the morphology or histology of the gastrointestinal tract and liver were observed. After 90 d of CBD exposure, a significant improvement in redox status was found in the blood plasma and liver. The concentration of malondialdehyde and carbonylated proteins was reduced compared to the control. In contrast to CBD, total oxidative stress was significantly increased and this was accompanied by an elevated level of malondialdehyde and carbonylated proteins in CBG-treated animals. Hepatotoxic (regressive changes) manifestations, disruption in white cell count, and alterations in the ALT activity, level of creatinine and ionized calcium were also found in CBG-treated animals. Based on liquid chromatography-mass spectrometry analysis, CBD/CBG accumulated in rat tissues (in the liver, brain, muscle, heart, kidney and skin) at a low ng level per gram. Both CBD and CBG molecular structures include a resorcinol moiety. In CBG, there is an extra dimethyloctadienyl structural pattern, which is most likely responsible for the disruption to the redox status and hepatic environment. The results are valuable to further investigation of the effects of CBD on redox status and should contribute towards opening up critical discussion on the applicability of other non-psychotropic cannabinoids.
ER  -

HOLCOVÁ POLANSKÁ, Hana, Kateřina PETRLÁKOVÁ, Barbora PAPOUSKOVA, Michal HENDRYCH, Amir SAMADIAN, Jan STORCH, Petr BABULA, Michal MASAŘÍK and Jan VACEK. Safety assessment and redox status in rats after chronic exposure to cannabidiol and cannabigerol. \textit{Toxicology}. CLARE: ELSEVIER IRELAND LTD, 2023, vol.~488, April 2023, p.~1-10. ISSN~0300-483X. Available from: https://dx.doi.org/10.1016/j.tox.2023.153460.

Detailed Information on Publication Record