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@article{2275821,
author = {Kacvinska, Katarina and Pavlinakova, Veronika and Polacek, Petr and Michlovska, Lenka and Hefka Blahnova, Veronika and Filova, Eva and Knoz, Martin and Lipový, Břetislav and Holoubek, Jakub and Faldyna, Martin and Pavlovský, Zdeněk and Vicenova, Monika and Cvanová, Michaela and Jarkovský, Jiří and Vojtova, Lucy},
article_location = {LONDON},
article_number = {1},
doi = {http://dx.doi.org/10.1186/s12951-023-01822-5},
keywords = {Bilayer; Chitosan; Collagen; Oxidized cellulose; Polydopamine; Wound healing},
language = {eng},
issn = {1477-3155},
journal = {JOURNAL OF NANOBIOTECHNOLOGY},
title = {Accelular nanofibrous bilayer scaffold intrapenetrated with polydopamine network and implemented into a full-thickness wound of a white-pig model affects inflammation and healing process},
url = {https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-023-01822-5},
volume = {21},
year = {2023}
}
TY - JOUR
ID - 2275821
AU - Kacvinska, Katarina - Pavlinakova, Veronika - Polacek, Petr - Michlovska, Lenka - Hefka Blahnova, Veronika - Filova, Eva - Knoz, Martin - Lipový, Břetislav - Holoubek, Jakub - Faldyna, Martin - Pavlovský, Zdeněk - Vicenova, Monika - Cvanová, Michaela - Jarkovský, Jiří - Vojtova, Lucy
PY - 2023
TI - Accelular nanofibrous bilayer scaffold intrapenetrated with polydopamine network and implemented into a full-thickness wound of a white-pig model affects inflammation and healing process
JF - JOURNAL OF NANOBIOTECHNOLOGY
VL - 21
IS - 1
SP - 1-24
EP - 1-24
PB - BIOMED CENTRAL LTD
SN - 14773155
KW - Bilayer
KW - Chitosan
KW - Collagen
KW - Oxidized cellulose
KW - Polydopamine
KW - Wound healing
UR - https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-023-01822-5
N2 - Treatment of complete loss of skin thickness requires expensive cellular materials and limited skin grafts used as temporary coverage. This paper presents an acellular bilayer scaffold modified with polydopamine (PDA), which is designed to mimic a missing dermis and a basement membrane (BM). The alternate dermis is made from freeze-dried collagen and chitosan (Coll/Chit) or collagen and a calcium salt of oxidized cellulose (Coll/CaOC). Alternate BM is made from electrospun gelatin (Gel), polycaprolactone (PCL), and CaOC. Morphological and mechanical analyzes have shown that PDA significantly improved the elasticity and strength of collagen microfibrils, which favorably affected swelling capacity and porosity. PDA significantly supported and maintained metabolic activity, proliferation, and viability of the murine fibroblast cell lines. The in vivo experiment carried out in a domestic Large white pig model resulted in the expression of pro-inflammatory cytokines in the first 1–2 weeks, giving the idea that PDA and/or CaOC trigger the early stages of inflammation. Otherwise, in later stages, PDA caused a reduction in inflammation with the expression of the anti-inflammatory molecule IL10 and the transforming growth factor β (TGFβ1), which could support the formation of fibroblasts. Similarities in treatment with native porcine skin suggested that the bilayer can be used as an implant for full-thickness skin wounds and thus eliminate the use of skin grafts.
ER -
KACVINSKA, Katarina, Veronika PAVLINAKOVA, Petr POLACEK, Lenka MICHLOVSKA, Veronika HEFKA BLAHNOVA, Eva FILOVA, Martin KNOZ, Břetislav LIPOVÝ, Jakub HOLOUBEK, Martin FALDYNA, Zdeněk PAVLOVSKÝ, Monika VICENOVA, Michaela CVANOVÁ, Jiří JARKOVSKÝ and Lucy VOJTOVA. Accelular nanofibrous bilayer scaffold intrapenetrated with polydopamine network and implemented into a full-thickness wound of a white-pig model affects inflammation and healing process. \textit{JOURNAL OF NANOBIOTECHNOLOGY}. LONDON: BIOMED CENTRAL LTD, 2023, vol.~21, No~1, p.~1-24. ISSN~1477-3155. Available from: https://dx.doi.org/10.1186/s12951-023-01822-5.
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