Mercury Content and Amelioration of Its Toxicity by Nitric
Oxide in Lichens

J 2023

Mercury Content and Amelioration of Its Toxicity by Nitric Oxide in Lichens

KOVACIK, Jozef, Lenka HUSAKOVA, Martina PIROUTKOVA and Petr BABULA

Basic information

Original name

Mercury Content and Amelioration of Its Toxicity by Nitric Oxide in Lichens

Authors

KOVACIK, Jozef (703 Slovakia, guarantor), Lenka HUSAKOVA (203 Czech Republic), Martina PIROUTKOVA (203 Czech Republic) and Petr BABULA (203 Czech Republic, belonging to the institution)

Edition

PLANTS-BASEL, BASEL, MDPI, 2023, 2223-7747

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30105 Physiology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.500 in 2022

RIV identification code

RIV/00216224:14110/23:00130702

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.3390/plants12040727

UT WoS

000940856900001

Keywords in English

antioxidants; biomonitoring; heavy metals; reactive oxygen species

Abstract

V originále

Mercury (Hg) content measured in five epiphytic lichen species collected in Slovakia mountain forests ranged from 30 to 100 ng/g DW and was species-specific, decreasing in the order Hypogymnia > Pseudevernia > Usnea > Xanthoria > Evernia prunastri (but polluted sites had no impact on Hg amount in Xanthoria). Evernia was therefore used to study the impact of short-term exogenous Hg (100 mu M, 24 h) and possible amelioration of Hg toxicity by nitric oxide (NO) donor sodium nitroprusside (SNP). NO was efficiently released from SNP as detected by two staining reagents and fluorescence microscopy and reduced Hg-induced ROS signal and absorption of Hg by thalli of Evernia prunastri. At the same time, NO ameliorated Hg-induced depletion of metabolites such as ascorbic acid and non-protein thiols, but not of free amino acids. The amount of metabolites, including soluble phenols, was reduced by excess Hg per se. On the contrary, NO was unable to restore Hg-stimulated depletion of chlorophyll autofluorescence but mitigated the decline of some macronutrients (K and Ca). Data confirm that accumulation of Hg in the epiphytic lichens is species-specific and that NO is a vital molecule in Evernia prunastri that provides protection against Hg-induced toxicity with considerable positive impact on metabolic changes.

Citovat

KOVACIK, Jozef, Lenka HUSAKOVA, Martina PIROUTKOVA and Petr BABULA. Mercury Content and Amelioration of Its Toxicity by Nitric Oxide in Lichens. PLANTS-BASEL. BASEL: MDPI, 2023, vol. 12, No 4, p. 1-12. ISSN 2223-7747. Available from: https://dx.doi.org/10.3390/plants12040727.

@article{2279479,
   author = {Kovacik, Jozef and Husakova, Lenka and Piroutkova, Martina and Babula, Petr},
   article_location = {BASEL},
   article_number = {4},
   doi = {http://dx.doi.org/10.3390/plants12040727},
   keywords = {antioxidants; biomonitoring; heavy metals; reactive oxygen species},
   language = {eng},
   issn = {2223-7747},
   journal = {PLANTS-BASEL},
   title = {Mercury Content and Amelioration of Its Toxicity by Nitric Oxide in Lichens},
   url = {https://www.mdpi.com/2223-7747/12/4/727},
   volume = {12},
   year = {2023}
}

TY  - JOUR
ID  - 2279479
AU  - Kovacik, Jozef - Husakova, Lenka - Piroutkova, Martina - Babula, Petr
PY  - 2023
TI  - Mercury Content and Amelioration of Its Toxicity by Nitric Oxide in Lichens
JF  - PLANTS-BASEL
VL  - 12
IS  - 4
SP  - 1-12
EP  - 1-12
PB  - MDPI
SN  - 22237747
KW  - antioxidants
KW  - biomonitoring
KW  - heavy metals
KW  - reactive oxygen species
UR  - https://www.mdpi.com/2223-7747/12/4/727
N2  - Mercury (Hg) content measured in five epiphytic lichen species collected in Slovakia mountain forests ranged from 30 to 100 ng/g DW and was species-specific, decreasing in the order Hypogymnia > Pseudevernia > Usnea > Xanthoria > Evernia prunastri (but polluted sites had no impact on Hg amount in Xanthoria). Evernia was therefore used to study the impact of short-term exogenous Hg (100 mu M, 24 h) and possible amelioration of Hg toxicity by nitric oxide (NO) donor sodium nitroprusside (SNP). NO was efficiently released from SNP as detected by two staining reagents and fluorescence microscopy and reduced Hg-induced ROS signal and absorption of Hg by thalli of Evernia prunastri. At the same time, NO ameliorated Hg-induced depletion of metabolites such as ascorbic acid and non-protein thiols, but not of free amino acids. The amount of metabolites, including soluble phenols, was reduced by excess Hg per se. On the contrary, NO was unable to restore Hg-stimulated depletion of chlorophyll autofluorescence but mitigated the decline of some macronutrients (K and Ca). Data confirm that accumulation of Hg in the epiphytic lichens is species-specific and that NO is a vital molecule in Evernia prunastri that provides protection against Hg-induced toxicity with considerable positive impact on metabolic changes.
ER  -

KOVACIK, Jozef, Lenka HUSAKOVA, Martina PIROUTKOVA and Petr BABULA. Mercury Content and Amelioration of Its Toxicity by Nitric Oxide in Lichens. \textit{PLANTS-BASEL}. BASEL: MDPI, 2023, vol.~12, No~4, p.~1-12. ISSN~2223-7747. Available from: https://dx.doi.org/10.3390/plants12040727.

Detailed Information on Publication Record