Preparation and Evaluation of a Dosage Form for Individualized
Administration of Lyophilized Probiotics

J 2023

Preparation and Evaluation of a Dosage Form for Individualized Administration of Lyophilized Probiotics

FÜLÖPOVÁ, Nicole, Natalia CHOMOVA, Jan ELBL, Dagmar MUDRONOVA, Patrik SIVULIČ et. al.

Basic information

Original name

Preparation and Evaluation of a Dosage Form for Individualized Administration of Lyophilized Probiotics

Authors

FÜLÖPOVÁ, Nicole (703 Slovakia, belonging to the institution), Natalia CHOMOVA, Jan ELBL (203 Czech Republic, belonging to the institution), Dagmar MUDRONOVA, Patrik SIVULIČ (703 Slovakia, belonging to the institution), Sylvie PAVLOKOVÁ (203 Czech Republic, guarantor, belonging to the institution) and Aleš FRANC (203 Czech Republic, belonging to the institution)

Edition

Pharmaceutics, Basel, MDPI, 2023, 1999-4923

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30104 Pharmacology and pharmacy

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.400 in 2022

RIV identification code

RIV/00216224:14160/23:00130704

Organization unit

Faculty of Pharmacy

DOI

http://dx.doi.org/10.3390/pharmaceutics15030910

UT WoS

000960126700001

Keywords in English

lyophilization; antropozoonoses; probiotic bacteria; individual treatment; principal component analysis; viability of bacteria

Abstract

V originále

Probiotics have been used in human and veterinary medicine to increase resistance to pathogens and provide protection against external impacts for many years. Pathogens are often transmitted to humans through animal product consumption. Therefore, it is assumed that probiotics protecting animals may also protect the humans who consume them. Many tested strains of probiotic bacteria can be used for individualized therapy. The recently isolated Lactobacillus plantarum R2 Biocenol™ has proven to be preferential in aquaculture, and potential benefits in humans are expected. A simple oral dosage form should be developed to test this hypothesis by a suitable preparation method, i.e., lyophilization, allowing the bacteria to survive longer. Lyophilizates were formed from silicates (Neusilin® NS2N; US2), cellulose derivates (Avicel® PH-101), and saccharides (inulin; saccharose; modified starch® 1500). They were evaluated for their physicochemical properties (pH leachate, moisture content, water absorption, wetting time, DSC tests, densities, and flow properties); their bacterial viability was determined in conditions including relevant studies over 6 months at 4 °C and scanned under an electron microscope. Lyophilizate composed of Neusilin® NS2N and saccharose appeared to be the most advantageous in terms of viability without any significant decrease. Its physicochemical properties are also suitable for capsule encapsulation, subsequent clinical evaluation, and individualized therapy.

Links

EF19_073/0016943, research and development project

Name: Interní grantová agentura Masarykovy univerzity

MUNI/IGA/0942/2021, interní kód MU

Name: Formulation of dosage form with probiotics (Lactobacillus) for an oral administration (Acronym: FODPOA)

Investor: Masaryk University

Citovat

FÜLÖPOVÁ, Nicole, Natalia CHOMOVA, Jan ELBL, Dagmar MUDRONOVA, Patrik SIVULIČ, Sylvie PAVLOKOVÁ and Aleš FRANC. Preparation and Evaluation of a Dosage Form for Individualized Administration of Lyophilized Probiotics. Pharmaceutics. Basel: MDPI, 2023, vol. 15, No 3, p. 1-19. ISSN 1999-4923. Available from: https://dx.doi.org/10.3390/pharmaceutics15030910.

@article{2279582,
   author = {Fülöpová, Nicole and Chomova, Natalia and Elbl, Jan and Mudronova, Dagmar and Sivulič, Patrik and Pavloková, Sylvie and Franc, Aleš},
   article_location = {Basel},
   article_number = {3},
   doi = {http://dx.doi.org/10.3390/pharmaceutics15030910},
   keywords = {lyophilization; antropozoonoses; probiotic bacteria; individual treatment; principal component analysis; viability of bacteria},
   language = {eng},
   issn = {1999-4923},
   journal = {Pharmaceutics},
   title = {Preparation and Evaluation of a Dosage Form for Individualized Administration of Lyophilized Probiotics},
   url = {https://www.mdpi.com/1999-4923/15/3/910},
   volume = {15},
   year = {2023}
}

TY  - JOUR
ID  - 2279582
AU  - Fülöpová, Nicole - Chomova, Natalia - Elbl, Jan - Mudronova, Dagmar - Sivulič, Patrik - Pavloková, Sylvie - Franc, Aleš
PY  - 2023
TI  - Preparation and Evaluation of a Dosage Form for Individualized Administration of Lyophilized Probiotics
JF  - Pharmaceutics
VL  - 15
IS  - 3
SP  - 1-19
EP  - 1-19
PB  - MDPI
SN  - 19994923
KW  - lyophilization
KW  - antropozoonoses
KW  - probiotic bacteria
KW  - individual treatment
KW  - principal component analysis
KW  - viability of bacteria
UR  - https://www.mdpi.com/1999-4923/15/3/910
N2  - Probiotics have been used in human and veterinary medicine to increase resistance to pathogens and provide protection against external impacts for many years. Pathogens are often transmitted to humans through animal product consumption. Therefore, it is assumed that probiotics protecting animals may also protect the humans who consume them. Many tested strains of probiotic bacteria can be used for individualized therapy. The recently isolated Lactobacillus plantarum R2 Biocenol™ has proven to be preferential in aquaculture, and potential benefits in humans are expected. A simple oral dosage form should be developed to test this hypothesis by a suitable preparation method, i.e., lyophilization, allowing the bacteria to survive longer. Lyophilizates were formed from silicates (Neusilin® NS2N; US2), cellulose derivates (Avicel® PH-101), and saccharides (inulin; saccharose; modified starch® 1500). They were evaluated for their physicochemical properties (pH leachate, moisture content, water absorption, wetting time, DSC tests, densities, and flow properties); their bacterial viability was determined in conditions including relevant studies over 6 months at 4 °C and scanned under an electron microscope. Lyophilizate composed of Neusilin® NS2N and saccharose appeared to be the most advantageous in terms of viability without any significant decrease. Its physicochemical properties are also suitable for capsule encapsulation, subsequent clinical evaluation, and individualized therapy.
ER  -

FÜLÖPOVÁ, Nicole, Natalia CHOMOVA, Jan ELBL, Dagmar MUDRONOVA, Patrik SIVULIČ, Sylvie PAVLOKOVÁ and Aleš FRANC. Preparation and Evaluation of a Dosage Form for Individualized Administration of Lyophilized Probiotics. \textit{Pharmaceutics}. Basel: MDPI, 2023, vol.~15, No~3, p.~1-19. ISSN~1999-4923. Available from: https://dx.doi.org/10.3390/pharmaceutics15030910.

Detailed Information on Publication Record