Labeling strategies for analysis of oligosaccharides and
glycans by capillary electrophoresis

J 2023

Labeling strategies for analysis of oligosaccharides and glycans by capillary electrophoresis

SMOLKOVÁ, Denisa, Richard ČMELÍK and Jana LAVICKÁ

Basic information

Original name

Labeling strategies for analysis of oligosaccharides and glycans by capillary electrophoresis

Authors

SMOLKOVÁ, Denisa (703 Slovakia, belonging to the institution), Richard ČMELÍK (203 Czech Republic) and Jana LAVICKÁ (203 Czech Republic, guarantor)

Edition

TrAC Trends in Analytical Chemistry, Elsevier B.V. 2023, 0165-9936

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 13.100 in 2022

RIV identification code

RIV/00216224:14310/23:00130705

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.trac.2023.117068

UT WoS

000991776100001

Keywords in English

Capillary electrophoresis; Derivatization; Fluorescence; Glycans; Glycomics; Labeling; Mass spectrometry; Oligosaccharides

Abstract

V originále

Capillary electrophoresis represents a powerful separation technique that has been widely accepted in oligosaccharide and glycan analysis. However, due to their low electrophoretic mobility as well as the lack of chromophore or fluorophore in their native structure, appropriate modification of the saccharide molecule is required before separation and detection. In this review, we summarize the labeling strategies used for oligosaccharide/glycan analysis followed by electrophoretic separation published between 2010 and mid-2022. The review is divided by the labeling mechanism and part of the saccharide molecule used for modification. Although most labeling for CE-based applications is done with 8-aminopyrene-1,3,6-trisulfonic acid trisodium salt, we have also summarized less common, rare, and very recently developed tags with applicability in glycomics.

Links

MUNI/A/1421/2022, interní kód MU

Name: Vývoj metod a instrumentace pro analýzu biologicky významných látek 2023

Investor: Masaryk University

Citovat

SMOLKOVÁ, Denisa, Richard ČMELÍK and Jana LAVICKÁ. Labeling strategies for analysis of oligosaccharides and glycans by capillary electrophoresis. TrAC Trends in Analytical Chemistry. Elsevier B.V., 2023, vol. 163, June, p. 1-9. ISSN 0165-9936. Available from: https://dx.doi.org/10.1016/j.trac.2023.117068.

@article{2279583,
   author = {Smolková, Denisa and Čmelík, Richard and Lavická, Jana},
   article_number = {June},
   doi = {http://dx.doi.org/10.1016/j.trac.2023.117068},
   keywords = {Capillary electrophoresis; Derivatization; Fluorescence; Glycans; Glycomics; Labeling; Mass spectrometry; Oligosaccharides},
   language = {eng},
   issn = {0165-9936},
   journal = {TrAC Trends in Analytical Chemistry},
   title = {Labeling strategies for analysis of oligosaccharides and glycans by capillary electrophoresis},
   url = {https://doi.org/10.1016/j.trac.2023.117068},
   volume = {163},
   year = {2023}
}

TY  - JOUR
ID  - 2279583
AU  - Smolková, Denisa - Čmelík, Richard - Lavická, Jana
PY  - 2023
TI  - Labeling strategies for analysis of oligosaccharides and glycans by capillary electrophoresis
JF  - TrAC Trends in Analytical Chemistry
VL  - 163
IS  - June
SP  - 1-9
EP  - 1-9
PB  - Elsevier B.V.
SN  - 01659936
KW  - Capillary electrophoresis
KW  - Derivatization
KW  - Fluorescence
KW  - Glycans
KW  - Glycomics
KW  - Labeling
KW  - Mass spectrometry
KW  - Oligosaccharides
UR  - https://doi.org/10.1016/j.trac.2023.117068
N2  - Capillary electrophoresis represents a powerful separation technique that has been widely accepted in oligosaccharide and glycan analysis. However, due to their low electrophoretic mobility as well as the lack of chromophore or fluorophore in their native structure, appropriate modification of the saccharide molecule is required before separation and detection. In this review, we summarize the labeling strategies used for oligosaccharide/glycan analysis followed by electrophoretic separation published between 2010 and mid-2022. The review is divided by the labeling mechanism and part of the saccharide molecule used for modification. Although most labeling for CE-based applications is done with 8-aminopyrene-1,3,6-trisulfonic acid trisodium salt, we have also summarized less common, rare, and very recently developed tags with applicability in glycomics.
ER  -

SMOLKOVÁ, Denisa, Richard ČMELÍK and Jana LAVICKÁ. Labeling strategies for analysis of oligosaccharides and glycans by capillary electrophoresis. \textit{TrAC Trends in Analytical Chemistry}. Elsevier B.V., 2023, vol.~163, June, p.~1-9. ISSN~0165-9936. Available from: https://dx.doi.org/10.1016/j.trac.2023.117068.

Detailed Information on Publication Record