Novel Förster Resonance Energy Transfer probe with quantum dot
for a long-time imaging of active caspases inside individual
cells

J 2023

Novel Förster Resonance Energy Transfer probe with quantum dot for a long-time imaging of active caspases inside individual cells

PROCHÁZKOVÁ, Markéta, Eliška KUCHOVSKÁ, Michael KILLINGER and Karel KLEPÁRNÍK

Basic information

Original name

Novel Förster Resonance Energy Transfer probe with quantum dot for a long-time imaging of active caspases inside individual cells

Authors

PROCHÁZKOVÁ, Markéta (203 Czech Republic, belonging to the institution), Eliška KUCHOVSKÁ (203 Czech Republic), Michael KILLINGER (203 Czech Republic, belonging to the institution) and Karel KLEPÁRNÍK (203 Czech Republic, guarantor)

Edition

Analytica Chimica Acta, Elsevier B.V. 2023, 0003-2670

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 6.200 in 2022

RIV identification code

RIV/00216224:14310/23:00130766

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.aca.2023.341334

UT WoS

001009667500001

Keywords in English

Caspase molecular probe; Time-laps fluorescence microscopy; Quantum dot photostability; FRET probe

Abstract

V originále

With the goal to investigate biological phenomena at a single-cell level, we designed, synthesized and tested a molecular probe based on Förster resonance energy transfer (FRET) between a highly luminescent quantum dot (QD) as a donor and a fluorophore or fluorescence quencher as an acceptor linked by a specific peptide. In principle, QD luminescence, effectively dissipated in the probe, is switched on after the cleavage of the peptide by a protease and the release of the quencher. We proposed a novel synthesis strategy of a probe. A two-step synthesis consists of: (i) Conjugation of CdTe QDs functionalized by –COOH groups of succinic acid on the nanoparticle surface with the designed specific peptide (GTADVEDTSC) using a ligand-exchange approach; (ii) A fast, high-yield reaction of amine-reactive succinimidyl group on the BHQ-2 quencher with N-terminal of the peptide.

Links

MUNI/A/1421/2022, interní kód MU

Name: Vývoj metod a instrumentace pro analýzu biologicky významných látek 2023

Investor: Masaryk University

Citovat

PROCHÁZKOVÁ, Markéta, Eliška KUCHOVSKÁ, Michael KILLINGER and Karel KLEPÁRNÍK. Novel Förster Resonance Energy Transfer probe with quantum dot for a long-time imaging of active caspases inside individual cells. Analytica Chimica Acta. Elsevier B.V., 2023, vol. 1267, August, p. 1-9. ISSN 0003-2670. Available from: https://dx.doi.org/10.1016/j.aca.2023.341334.

@article{2282018,
   author = {Procházková, Markéta and Kuchovská, Eliška and Killinger, Michael and Klepárník, Karel},
   article_number = {August},
   doi = {http://dx.doi.org/10.1016/j.aca.2023.341334},
   keywords = {Caspase molecular probe; Time-laps fluorescence microscopy; Quantum dot photostability; FRET probe},
   language = {eng},
   issn = {0003-2670},
   journal = {Analytica Chimica Acta},
   title = {Novel Förster Resonance Energy Transfer probe with quantum dot for a long-time imaging of active caspases inside individual cells},
   url = {https://www.sciencedirect.com/science/article/pii/S000326702300555X},
   volume = {1267},
   year = {2023}
}

TY  - JOUR
ID  - 2282018
AU  - Procházková, Markéta - Kuchovská, Eliška - Killinger, Michael - Klepárník, Karel
PY  - 2023
TI  - Novel Förster Resonance Energy Transfer probe with quantum dot for a long-time imaging of active caspases inside individual cells
JF  - Analytica Chimica Acta
VL  - 1267
IS  - August
SP  - 1-9
EP  - 1-9
PB  - Elsevier B.V.
SN  - 00032670
KW  - Caspase molecular probe
KW  - Time-laps fluorescence microscopy
KW  - Quantum dot photostability
KW  - FRET probe
UR  - https://www.sciencedirect.com/science/article/pii/S000326702300555X
N2  - With the goal to investigate biological phenomena at a single-cell level, we designed, synthesized and tested a molecular probe based on Förster resonance energy transfer (FRET) between a highly luminescent quantum dot (QD) as a donor and a fluorophore or fluorescence quencher as an acceptor linked by a specific peptide. In principle, QD luminescence, effectively dissipated in the probe, is switched on after the cleavage of the peptide by a protease and the release of the quencher. We proposed a novel synthesis strategy of a probe. A two-step synthesis consists of: (i) Conjugation of CdTe QDs functionalized by –COOH groups of succinic acid on the nanoparticle surface with the designed specific peptide (GTADVEDTSC) using a ligand-exchange approach; (ii) A fast, high-yield reaction of amine-reactive succinimidyl group on the BHQ-2 quencher with N-terminal of the peptide.
ER  -

PROCHÁZKOVÁ, Markéta, Eliška KUCHOVSKÁ, Michael KILLINGER and Karel KLEPÁRNÍK. Novel Förster Resonance Energy Transfer probe with quantum dot for a long-time imaging of active caspases inside individual cells. \textit{Analytica Chimica Acta}. Elsevier B.V., 2023, vol.~1267, August, p.~1-9. ISSN~0003-2670. Available from: https://dx.doi.org/10.1016/j.aca.2023.341334.

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