Comprehensive analysis of floral scent and fatty acids in
nectar of Silene nutans through modern analytical gas
chromatography techniques

J 2023

Comprehensive analysis of floral scent and fatty acids in nectar of Silene nutans through modern analytical gas chromatography techniques

SPADAFORA, Natasha Damiana, Damien EGGERMONT, Veronika KŘEŠŤÁKOVÁ, Tatiana CHENET, Fabienne VAN ROSSUM et. al.

Basic information

Original name

Comprehensive analysis of floral scent and fatty acids in nectar of Silene nutans through modern analytical gas chromatography techniques

Authors

SPADAFORA, Natasha Damiana, Damien EGGERMONT, Veronika KŘEŠŤÁKOVÁ (203 Czech Republic, belonging to the institution), Tatiana CHENET, Fabienne VAN ROSSUM and Giorgia PURCARO (guarantor)

Edition

Journal of Chromatography A, Elsevier, 2023, 0021-9673

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.100 in 2022

RIV identification code

RIV/00216224:14310/23:00130780

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.chroma.2023.463977

UT WoS

000982832600001

Keywords in English

Head-space extraction; Chemometrics; GC × GC; Fatty acids; Volatile organic compounds

Abstract

V originále

The aim of this work was to show the potential of multidimensional gas chromatography combined with mass spectrometry and suitable chemometrics means based on untargeted and profiling data analysis to strengthen the information provided by floral scent and nectar fatty acids of four genetically differentiated lineages (E1, W1, W2, and W3) of the nocturnal moth-pollinated herb Silene nutans. Volatile organic compounds emitted by flowers were trapped for a total of 42 samples by in-vivo sampling dynamic head space for analysing floral scent by untargeted approach, while 37 samples of nectar were collected for analysing fatty acids through profiling analysis. The resulting data from floral scent analysis were aligned and compared using a tile-based methodology followed by data mining to access high-level information. Based on floral scent and nectar fatty acid results, it was possible to distinguish E1 from the W lineages, and W3 from W1 and W2. This work puts the bases for a larger study aiming to clarify the existence of prezygotic barriers involved in speciation among lineages of S. nutans, and thus the possible implication of different flower scents and nectar compositions in this phenomenon.

Citovat

SPADAFORA, Natasha Damiana, Damien EGGERMONT, Veronika KŘEŠŤÁKOVÁ, Tatiana CHENET, Fabienne VAN ROSSUM and Giorgia PURCARO. Comprehensive analysis of floral scent and fatty acids in nectar of Silene nutans through modern analytical gas chromatography techniques. Journal of Chromatography A. Elsevier, 2023, vol. 1696, May, p. 1-9. ISSN 0021-9673. Available from: https://dx.doi.org/10.1016/j.chroma.2023.463977.

@article{2282780,
   author = {Spadafora, Natasha Damiana and Eggermont, Damien and Křešťáková, Veronika and Chenet, Tatiana and Van Rossum, Fabienne and Purcaro, Giorgia},
   article_number = {May},
   doi = {http://dx.doi.org/10.1016/j.chroma.2023.463977},
   keywords = {Head-space extraction; Chemometrics; GC × GC; Fatty acids; Volatile organic compounds},
   language = {eng},
   issn = {0021-9673},
   journal = {Journal of Chromatography A},
   title = {Comprehensive analysis of floral scent and fatty acids in nectar of Silene nutans through modern analytical gas chromatography techniques},
   url = {https://doi.org/10.1016/j.chroma.2023.463977},
   volume = {1696},
   year = {2023}
}

TY  - JOUR
ID  - 2282780
AU  - Spadafora, Natasha Damiana - Eggermont, Damien - Křešťáková, Veronika - Chenet, Tatiana - Van Rossum, Fabienne - Purcaro, Giorgia
PY  - 2023
TI  - Comprehensive analysis of floral scent and fatty acids in nectar of Silene nutans through modern analytical gas chromatography techniques
JF  - Journal of Chromatography A
VL  - 1696
IS  - May
SP  - 1-9
EP  - 1-9
PB  - Elsevier
SN  - 00219673
KW  - Head-space extraction
KW  - Chemometrics
KW  - GC × GC
KW  - Fatty acids
KW  - Volatile organic compounds
UR  - https://doi.org/10.1016/j.chroma.2023.463977
N2  - The aim of this work was to show the potential of multidimensional gas chromatography combined with mass spectrometry and suitable chemometrics means based on untargeted and profiling data analysis to strengthen the information provided by floral scent and nectar fatty acids of four genetically differentiated lineages (E1, W1, W2, and W3) of the nocturnal moth-pollinated herb Silene nutans. Volatile organic compounds emitted by flowers were trapped for a total of 42 samples by in-vivo sampling dynamic head space for analysing floral scent by untargeted approach, while 37 samples of nectar were collected for analysing fatty acids through profiling analysis. The resulting data from floral scent analysis were aligned and compared using a tile-based methodology followed by data mining to access high-level information. Based on floral scent and nectar fatty acid results, it was possible to distinguish E1 from the W lineages, and W3 from W1 and W2. This work puts the bases for a larger study aiming to clarify the existence of prezygotic barriers involved in speciation among lineages of S. nutans, and thus the possible implication of different flower scents and nectar compositions in this phenomenon.
ER  -

SPADAFORA, Natasha Damiana, Damien EGGERMONT, Veronika KŘEŠŤÁKOVÁ, Tatiana CHENET, Fabienne VAN ROSSUM and Giorgia PURCARO. Comprehensive analysis of floral scent and fatty acids in nectar of Silene nutans through modern analytical gas chromatography techniques. \textit{Journal of Chromatography A}. Elsevier, 2023, vol.~1696, May, p.~1-9. ISSN~0021-9673. Available from: https://dx.doi.org/10.1016/j.chroma.2023.463977.

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