Does dietary exposure to 17?-ethinylestradiol alter biomarkers related with endocrine disruption and oxidative stress in the adult triploid of Danio rerio?

WEISEROVA, Zuzana, Jana BLAHOVA, Veronika DOBUKOVA, Petr MARSALEK, Nikola HODKOVICOVA, Jiri LENZ, Frantisek TICHY, Roman FRANEK, Martin PSENICKA, Aleš FRANC and Zdenka SVOBODOVA. Does dietary exposure to 17?-ethinylestradiol alter biomarkers related with endocrine disruption and oxidative stress in the adult triploid of Danio rerio? Science of the Total Environment. Amsterdam: Elsevier, 2023, vol. 870, April, p. 1-11. ISSN 0048-9697. Available from: https://dx.doi.org/10.1016/j.scitotenv.2023.161911.

Basic information

• Original name: Does dietary exposure to 17?-ethinylestradiol alter biomarkers related with endocrine disruption and oxidative stress in the adult triploid of Danio rerio?
• Authors: WEISEROVA, Zuzana, Jana BLAHOVA (guarantor), Veronika DOBUKOVA, Petr MARSALEK, Nikola HODKOVICOVA, Jiri LENZ, Frantisek TICHY, Roman FRANEK, Martin PSENICKA, Aleš FRANC (203 Czech Republic, belonging to the institution) and Zdenka SVOBODOVA.
• Edition: Science of the Total Environment, Amsterdam, Elsevier, 2023, 0048-9697.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 30104 Pharmacology and pharmacy
• Country of publisher: Netherlands
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 9.800 in 2022
• RIV identification code: RIV/00216224:14160/23:00130832
• Organization unit: Faculty of Pharmacy
• Doi: http://dx.doi.org/10.1016/j.scitotenv.2023.161911
• UT WoS: 000963644800001
• Keywords in English: Estrogens; Zebrafish; Male; Vitellogenin; Gene expression; Antioxidant and detoxifying enzymes
• Tags: rivok, ÚFT
• Tags: International impact, Reviewed

Abstract

This study was conducted to investigate a comprehensive effect of 17 alpha-ethinylestradiol (EE2) in zebrafish (Danio rerio) with the emphasis on endocrine disruption, oxidative stress and detoxification processes at different levels. Adult male triploid zebrafish were exposed to EE2 administered in feed at two concentrations - 10 and 1000 mu g/kg for six weeks. The estrogenic potential of EE2 was evaluated using an analysis of vitellogenin, gene expression focused on reproduc-tive disorders and gonad histological examination. The alterations in antioxidant and detoxification status were assessed using analyses of enzyme activities and changes in transcriptional levels of selected genes. The most signifi-cant changes were observed especially in fish exposed to a high concentration of EE2 (i.e., 1000 mu g/kg). Such high con-centration caused extensive mortality (25 %) mainly in the second half of the experiment followed by a highly significant decrease in the length and body weight. Similarly, highly significant induction of vitellogenin level and vtg1 mRNA expression (about 43,000-fold compared to the control) as well as a significant downregulation of gonad aromatase expression (cyp19a1a) and histological changes in testicular tissue were confirmed in this group. In the group exposed to environmentally relevant concentration of EE2 (i.e., 10 mu g/kg), no significant differences in vitello-genin were observed, although all fish were positive in the detection of vitellogenin compared to control, where only 40 % of individuals were positive. In addition, the high concentration of EE2 resulted in significant alterations in most monitored antioxidant and detoxifying enzymes with the exception of catalase, followed by strongly significant upregulation in mRNA expression of gsr, gpx1a, cat and cyp1a genes. Furthermore, a significant decrease in the glutathione reductase activity was recorded in fish exposed to 10 mu g EE2/kg. To our knowledge, this is the first study which reports the effects of subchronic per oral exposure to EE2 in adult triploid zebrafish.