Does dietary exposure to 17?-ethinylestradiol alter biomarkers
related with endocrine disruption and oxidative stress in the
adult triploid of Danio rerio?

J 2023

Does dietary exposure to 17?-ethinylestradiol alter biomarkers related with endocrine disruption and oxidative stress in the adult triploid of Danio rerio?

WEISEROVA, Zuzana, Jana BLAHOVA, Veronika DOBUKOVA, Petr MARSALEK, Nikola HODKOVICOVA et. al.

Basic information

Original name

Does dietary exposure to 17?-ethinylestradiol alter biomarkers related with endocrine disruption and oxidative stress in the adult triploid of Danio rerio?

Authors

WEISEROVA, Zuzana, Jana BLAHOVA (guarantor), Veronika DOBUKOVA, Petr MARSALEK, Nikola HODKOVICOVA, Jiri LENZ, Frantisek TICHY, Roman FRANEK, Martin PSENICKA, Aleš FRANC (203 Czech Republic, belonging to the institution) and Zdenka SVOBODOVA

Edition

Science of the Total Environment, Amsterdam, Elsevier, 2023, 0048-9697

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30104 Pharmacology and pharmacy

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 9.800 in 2022

RIV identification code

RIV/00216224:14160/23:00130832

Organization unit

Faculty of Pharmacy

DOI

http://dx.doi.org/10.1016/j.scitotenv.2023.161911

UT WoS

000963644800001

Keywords in English

Estrogens; Zebrafish; Male; Vitellogenin; Gene expression; Antioxidant and detoxifying enzymes

Abstract

V originále

This study was conducted to investigate a comprehensive effect of 17 alpha-ethinylestradiol (EE2) in zebrafish (Danio rerio) with the emphasis on endocrine disruption, oxidative stress and detoxification processes at different levels. Adult male triploid zebrafish were exposed to EE2 administered in feed at two concentrations - 10 and 1000 mu g/kg for six weeks. The estrogenic potential of EE2 was evaluated using an analysis of vitellogenin, gene expression focused on reproduc-tive disorders and gonad histological examination. The alterations in antioxidant and detoxification status were assessed using analyses of enzyme activities and changes in transcriptional levels of selected genes. The most signifi-cant changes were observed especially in fish exposed to a high concentration of EE2 (i.e., 1000 mu g/kg). Such high con-centration caused extensive mortality (25 %) mainly in the second half of the experiment followed by a highly significant decrease in the length and body weight. Similarly, highly significant induction of vitellogenin level and vtg1 mRNA expression (about 43,000-fold compared to the control) as well as a significant downregulation of gonad aromatase expression (cyp19a1a) and histological changes in testicular tissue were confirmed in this group. In the group exposed to environmentally relevant concentration of EE2 (i.e., 10 mu g/kg), no significant differences in vitello-genin were observed, although all fish were positive in the detection of vitellogenin compared to control, where only 40 % of individuals were positive. In addition, the high concentration of EE2 resulted in significant alterations in most monitored antioxidant and detoxifying enzymes with the exception of catalase, followed by strongly significant upregulation in mRNA expression of gsr, gpx1a, cat and cyp1a genes. Furthermore, a significant decrease in the glutathione reductase activity was recorded in fish exposed to 10 mu g EE2/kg. To our knowledge, this is the first study which reports the effects of subchronic per oral exposure to EE2 in adult triploid zebrafish.

Citovat

WEISEROVA, Zuzana, Jana BLAHOVA, Veronika DOBUKOVA, Petr MARSALEK, Nikola HODKOVICOVA, Jiri LENZ, Frantisek TICHY, Roman FRANEK, Martin PSENICKA, Aleš FRANC and Zdenka SVOBODOVA. Does dietary exposure to 17?-ethinylestradiol alter biomarkers related with endocrine disruption and oxidative stress in the adult triploid of Danio rerio? Science of the Total Environment. Amsterdam: Elsevier, 2023, vol. 870, April, p. 1-11. ISSN 0048-9697. Available from: https://dx.doi.org/10.1016/j.scitotenv.2023.161911.

@article{2285497,
   author = {Weiserova, Zuzana and Blahova, Jana and Dobukova, Veronika and Marsalek, Petr and Hodkovicova, Nikola and Lenz, Jiri and Tichy, Frantisek and Franek, Roman and Psenicka, Martin and Franc, Aleš and Svobodova, Zdenka},
   article_location = {Amsterdam},
   article_number = {April},
   doi = {http://dx.doi.org/10.1016/j.scitotenv.2023.161911},
   keywords = {Estrogens; Zebrafish; Male; Vitellogenin; Gene expression; Antioxidant and detoxifying enzymes},
   language = {eng},
   issn = {0048-9697},
   journal = {Science of the Total Environment},
   title = {Does dietary exposure to 17?-ethinylestradiol alter biomarkers related with endocrine disruption and oxidative stress in the adult triploid of Danio rerio?},
   url = {https://www.sciencedirect.com/science/article/pii/S0048969723005260?via%3Dihub},
   volume = {870},
   year = {2023}
}

TY  - JOUR
ID  - 2285497
AU  - Weiserova, Zuzana - Blahova, Jana - Dobukova, Veronika - Marsalek, Petr - Hodkovicova, Nikola - Lenz, Jiri - Tichy, Frantisek - Franek, Roman - Psenicka, Martin - Franc, Aleš - Svobodova, Zdenka
PY  - 2023
TI  - Does dietary exposure to 17?-ethinylestradiol alter biomarkers related with endocrine disruption and oxidative stress in the adult triploid of Danio rerio?
JF  - Science of the Total Environment
VL  - 870
IS  - April
SP  - 1-11
EP  - 1-11
PB  - Elsevier
SN  - 00489697
KW  - Estrogens
KW  - Zebrafish
KW  - Male
KW  - Vitellogenin
KW  - Gene expression
KW  - Antioxidant and detoxifying enzymes
UR  - https://www.sciencedirect.com/science/article/pii/S0048969723005260?via%3Dihub
N2  - This study was conducted to investigate a comprehensive effect of 17 alpha-ethinylestradiol (EE2) in zebrafish (Danio rerio) with the emphasis on endocrine disruption, oxidative stress and detoxification processes at different levels. Adult male triploid zebrafish were exposed to EE2 administered in feed at two concentrations - 10 and 1000 mu g/kg for six weeks. The estrogenic potential of EE2 was evaluated using an analysis of vitellogenin, gene expression focused on reproduc-tive disorders and gonad histological examination. The alterations in antioxidant and detoxification status were assessed using analyses of enzyme activities and changes in transcriptional levels of selected genes. The most signifi-cant changes were observed especially in fish exposed to a high concentration of EE2 (i.e., 1000 mu g/kg). Such high con-centration caused extensive mortality (25 %) mainly in the second half of the experiment followed by a highly significant decrease in the length and body weight. Similarly, highly significant induction of vitellogenin level and vtg1 mRNA expression (about 43,000-fold compared to the control) as well as a significant downregulation of gonad aromatase expression (cyp19a1a) and histological changes in testicular tissue were confirmed in this group. In the group exposed to environmentally relevant concentration of EE2 (i.e., 10 mu g/kg), no significant differences in vitello-genin were observed, although all fish were positive in the detection of vitellogenin compared to control, where only 40 % of individuals were positive. In addition, the high concentration of EE2 resulted in significant alterations in most monitored antioxidant and detoxifying enzymes with the exception of catalase, followed by strongly significant upregulation in mRNA expression of gsr, gpx1a, cat and cyp1a genes. Furthermore, a significant decrease in the glutathione reductase activity was recorded in fish exposed to 10 mu g EE2/kg. To our knowledge, this is the first study which reports the effects of subchronic per oral exposure to EE2 in adult triploid zebrafish.
ER  -

WEISEROVA, Zuzana, Jana BLAHOVA, Veronika DOBUKOVA, Petr MARSALEK, Nikola HODKOVICOVA, Jiri LENZ, Frantisek TICHY, Roman FRANEK, Martin PSENICKA, Aleš FRANC and Zdenka SVOBODOVA. Does dietary exposure to 17?-ethinylestradiol alter biomarkers related with endocrine disruption and oxidative stress in the adult triploid of Danio rerio? \textit{Science of the Total Environment}. Amsterdam: Elsevier, 2023, vol.~870, April, p.~1-11. ISSN~0048-9697. Available from: https://dx.doi.org/10.1016/j.scitotenv.2023.161911.

Detailed Information on Publication Record