Pseudomonas petrae sp. nov. isolated from regolith samples in
Antarctica

J 2023

Pseudomonas petrae sp. nov. isolated from regolith samples in Antarctica

NOVÁKOVÁ, Dana, Vendula KOUBLOVÁ, Karel SEDLÁŘ, Eva STAŇKOVÁ, Stanislava KRÁLOVÁ et. al.

Basic information

Original name

Pseudomonas petrae sp. nov. isolated from regolith samples in Antarctica

Authors

NOVÁKOVÁ, Dana (203 Czech Republic, guarantor, belonging to the institution), Vendula KOUBLOVÁ (203 Czech Republic, belonging to the institution), Karel SEDLÁŘ, Eva STAŇKOVÁ (203 Czech Republic, belonging to the institution), Stanislava KRÁLOVÁ (703 Slovakia, belonging to the institution), Pavel ŠVEC (203 Czech Republic, belonging to the institution), Meina NEUMANN-SCHAAL, Jacqueline WOLF, Sylva KOUDELKOVÁ (203 Czech Republic, belonging to the institution), Miloš BARTÁK (203 Czech Republic, belonging to the institution) and Ivo SEDLÁČEK (203 Czech Republic, belonging to the institution)

Edition

Systematic and Applied Microbiology, Elsevier GmbH, 2023, 0723-2020

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

10606 Microbiology

Country of publisher

Germany

Confidentiality degree

is not subject to a state or trade secret

References:

URL

Impact factor

Impact factor: 3.300

RIV identification code

RIV/00216224:14310/23:00130840

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.syapm.2023.126424

UT WoS

001001607000001

Keywords in English

Pseudomonas; P. petrae sp. nov.; Antarctica; Regolith; Species description; Taxonomy

Abstract

V originále

A polyphasic taxonomic approach was used to characterize the four strains P2653T, P2652, P2498, and P2647, isolated from Antarctic regolith samples. Initial genotype screening performed by PCR fingerprinting based on repetitive sequences showed that the isolates studied formed a coherent cluster separated from the other Pseudomonas species. Identification results based on 16S rRNA gene sequences showed the highest sequence similarity with Pseudomonas graminis (99.7%), which was confirmed by multilocus sequence analysis using the rpoB, rpoD, and gyrB genes. Genome sequence comparison of P2653T with the most related P. graminis type strain DSM 11363T revealed an average nucleotide identity of 92.1% and a digital DNA-DNA hybridization value of 46.6%. The major fatty acids for all Antarctic strains were C16:0, Summed Feature 3 (C16:1 ω7c/C16:1 ω6c) and Summed Feature 8 (C18:1 ω7c/C18:1 ω6c). The predominant respiratory quinone was Q-9, and the major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, and phosphatidylglycerol. The regolith strains could be differentiated from related species by the absence of arginine dihydrolase, ornithine and lysine decarboxylase and by negative tyrosine hydrolysis. The results of this polyphasic study allowed the genotypic and phenotypic differentiation of four analysed strains from the closest related species, which confirmed that the strains represent a novel species within the genus Pseudomonas, for which the name Pseudomonas petrae sp. nov. is proposed with P2653T (CCM 8850T = DSM 112068T = LMG 30619T) as the type strain.

Links

EF18_046/0015974, research and development project

Name: Modernizace České infrastruktury pro integrativní strukturní biologii

LM2015078, research and development project

Name: Česká polární výzkumná infrastruktura (Acronym: CzechPolar2)

Investor: Ministry of Education, Youth and Sports of the CR

LM2018127, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

90242, large research infrastructures

Name: CIISB III

Citovat

NOVÁKOVÁ, Dana, Vendula KOUBLOVÁ, Karel SEDLÁŘ, Eva STAŇKOVÁ, Stanislava KRÁLOVÁ, Pavel ŠVEC, Meina NEUMANN-SCHAAL, Jacqueline WOLF, Sylva KOUDELKOVÁ, Miloš BARTÁK and Ivo SEDLÁČEK. Pseudomonas petrae sp. nov. isolated from regolith samples in Antarctica. Systematic and Applied Microbiology. Elsevier GmbH, 2023, vol. 46, No 4, p. 1-9. ISSN 0723-2020. Available from: https://dx.doi.org/10.1016/j.syapm.2023.126424.

@article{2285657,
   author = {Nováková, Dana and Koublová, Vendula and Sedlář, Karel and Staňková, Eva and Králová, Stanislava and Švec, Pavel and NeumannandSchaal, Meina and Wolf, Jacqueline and Koudelková, Sylva and Barták, Miloš and Sedláček, Ivo},
   article_number = {4},
   doi = {http://dx.doi.org/10.1016/j.syapm.2023.126424},
   keywords = {Pseudomonas; P. petrae sp. nov.; Antarctica; Regolith; Species description; Taxonomy},
   language = {eng},
   issn = {0723-2020},
   journal = {Systematic and Applied Microbiology},
   title = {Pseudomonas petrae sp. nov. isolated from regolith samples in Antarctica},
   url = {https://doi.org/10.1016/j.syapm.2023.126424},
   volume = {46},
   year = {2023}
}

TY  - JOUR
ID  - 2285657
AU  - Nováková, Dana - Koublová, Vendula - Sedlář, Karel - Staňková, Eva - Králová, Stanislava - Švec, Pavel - Neumann-Schaal, Meina - Wolf, Jacqueline - Koudelková, Sylva - Barták, Miloš - Sedláček, Ivo
PY  - 2023
TI  - Pseudomonas petrae sp. nov. isolated from regolith samples in Antarctica
JF  - Systematic and Applied Microbiology
VL  - 46
IS  - 4
SP  - 1-9
EP  - 1-9
PB  - Elsevier GmbH
SN  - 07232020
KW  - Pseudomonas
KW  - P. petrae sp. nov.
KW  - Antarctica
KW  - Regolith
KW  - Species description
KW  - Taxonomy
UR  - https://doi.org/10.1016/j.syapm.2023.126424
N2  - A polyphasic taxonomic approach was used to characterize the four strains P2653T, P2652, P2498, and P2647, isolated from Antarctic regolith samples. Initial genotype screening performed by PCR fingerprinting based on repetitive sequences showed that the isolates studied formed a coherent cluster separated from the other Pseudomonas species. Identification results based on 16S rRNA gene sequences showed the highest sequence similarity with Pseudomonas graminis (99.7%), which was confirmed by multilocus sequence analysis using the rpoB, rpoD, and gyrB genes. Genome sequence comparison of P2653T with the most related P. graminis type strain DSM 11363T revealed an average nucleotide identity of 92.1% and a digital DNA-DNA hybridization value of 46.6%. The major fatty acids for all Antarctic strains were C16:0, Summed Feature 3 (C16:1 ω7c/C16:1 ω6c) and Summed Feature 8 (C18:1 ω7c/C18:1 ω6c). The predominant respiratory quinone was Q-9, and the major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, and phosphatidylglycerol. The regolith strains could be differentiated from related species by the absence of arginine dihydrolase, ornithine and lysine decarboxylase and by negative tyrosine hydrolysis. The results of this polyphasic study allowed the genotypic and phenotypic differentiation of four analysed strains from the closest related species, which confirmed that the strains represent a novel species within the genus Pseudomonas, for which the name Pseudomonas petrae sp. nov. is proposed with P2653T (CCM 8850T = DSM 112068T = LMG 30619T) as the type strain.
ER  -

NOVÁKOVÁ, Dana, Vendula KOUBLOVÁ, Karel SEDLÁŘ, Eva STAŇKOVÁ, Stanislava KRÁLOVÁ, Pavel ŠVEC, Meina NEUMANN-SCHAAL, Jacqueline WOLF, Sylva KOUDELKOVÁ, Miloš BARTÁK and Ivo SEDLÁČEK. Pseudomonas petrae sp. nov. isolated from regolith samples in Antarctica. \textit{Systematic and Applied Microbiology}. Elsevier GmbH, 2023, vol.~46, No~4, p.~1-9. ISSN~0723-2020. Available from: https://dx.doi.org/10.1016/j.syapm.2023.126424.

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