Magnetically Driven Self-Degrading Zinc-Containing Cystine
Microrobots for Treatment of Prostate Cancer

J 2023

Magnetically Driven Self-Degrading Zinc-Containing Cystine Microrobots for Treatment of Prostate Cancer

USSIA, Martina, Mario URSO, Monika KRATOCHVÍLOVÁ, Jiří NAVRÁTIL, Jan BALVAN et. al.

Basic information

Original name

Magnetically Driven Self-Degrading Zinc-Containing Cystine Microrobots for Treatment of Prostate Cancer

Authors

USSIA, Martina, Mario URSO, Monika KRATOCHVÍLOVÁ (203 Czech Republic, belonging to the institution), Jiří NAVRÁTIL (203 Czech Republic, belonging to the institution), Jan BALVAN (203 Czech Republic, belonging to the institution), Carmen C. C. MAYORGA-MARTINEZ, Jan VYSKOCIL (203 Czech Republic), Michal MASAŘÍK (203 Czech Republic, belonging to the institution) and Martin PUMERA (203 Czech Republic, guarantor)

Edition

SMALL, WEINHEIM, WILEY-V C H VERLAG GMBH, 2023, 1613-6810

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30204 Oncology

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 13.300 in 2022

RIV identification code

RIV/00216224:14110/23:00131017

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1002/smll.202208259

UT WoS

000921348000001

Keywords in English

cysteine; magnetic actuation; micromotors; nanorobots; self-propulsion; tumors

Abstract

V originále

Prostate cancer is the most commonly diagnosed tumor disease in men, and its treatment is still a big challenge in standard oncology therapy. Magnetically actuated microrobots represent the most promising technology in modern nanomedicine, offering the advantage of wireless guidance, effective cell penetration, and non-invasive actuation. Here, new biodegradable magnetically actuated zinc/cystine-based microrobots for in situ treatment of prostate cancer cells are reported. The microrobots are fabricated via metal-ion-mediated self-assembly of the amino acid cystine encapsulating superparamagnetic Fe3O4 nanoparticles (NPs) during the synthesis, which allows their precise manipulation by a rotating magnetic field. Inside the cells, the typical enzymatic reducing environment favors the disassembly of the aminoacidic chemical structure due to the cleavage of cystine disulfide bonds and disruption of non-covalent interactions with the metal ions, as demonstrated by in vitro experiments with reduced nicotinamide adenine dinucleotide (NADH). In this way, the cystine microrobots served for site-specific delivery of Zn2+ ions responsible for tumor cell killing via a "Trojan horse effect". This work presents a new concept of cell internalization exploiting robotic systems' self-degradation, proposing a step forward in non-invasive cancer therapy.

Links

EF19_073/0016943, research and development project

Name: Interní grantová agentura Masarykovy univerzity

MUNI/A/1343/2022, interní kód MU

Name: Zátěže kardiovaskulárního systému od A po Z

Investor: Masaryk University, Loads on the cardiovascular system from A to Z

MUNI/A/1370/2022, interní kód MU

Name: Patofyziologie vybraných komplexních nemocí od molekulární do systémovou úroveň

Investor: Masaryk University, Pathophysiology of selected complex diseases from molecular to systemic level

MUNI/IGA/0954/2021, interní kód MU

Name: Mechanobiology in prostate cancer: Tumour aggressiveness associated with cell stiffness

Investor: Masaryk University

NU21-08-00407, research and development project

Name: Funkční nanoroboti pro navigovanou kombinovanou nádorovou terapii

Investor: Ministry of Health of the CR, Subprogram 1 - standard

90110, large research infrastructures

Name: CzechNanoLab

Citovat

USSIA, Martina, Mario URSO, Monika KRATOCHVÍLOVÁ, Jiří NAVRÁTIL, Jan BALVAN, Carmen C. C. MAYORGA-MARTINEZ, Jan VYSKOCIL, Michal MASAŘÍK and Martin PUMERA. Magnetically Driven Self-Degrading Zinc-Containing Cystine Microrobots for Treatment of Prostate Cancer. SMALL. WEINHEIM: WILEY-V C H VERLAG GMBH, 2023, vol. 19, No 17, p. 1-12. ISSN 1613-6810. Available from: https://dx.doi.org/10.1002/smll.202208259.

@article{2292957,
   author = {Ussia, Martina and Urso, Mario and Kratochvílová, Monika and Navrátil, Jiří and Balvan, Jan and MayorgaandMartinez, Carmen C. C. and Vyskocil, Jan and Masařík, Michal and Pumera, Martin},
   article_location = {WEINHEIM},
   article_number = {17},
   doi = {http://dx.doi.org/10.1002/smll.202208259},
   keywords = {cysteine; magnetic actuation; micromotors; nanorobots; self-propulsion; tumors},
   language = {eng},
   issn = {1613-6810},
   journal = {SMALL},
   title = {Magnetically Driven Self-Degrading Zinc-Containing Cystine Microrobots for Treatment of Prostate Cancer},
   url = {https://onlinelibrary.wiley.com/doi/10.1002/smll.202208259},
   volume = {19},
   year = {2023}
}

TY  - JOUR
ID  - 2292957
AU  - Ussia, Martina - Urso, Mario - Kratochvílová, Monika - Navrátil, Jiří - Balvan, Jan - Mayorga-Martinez, Carmen C. C. - Vyskocil, Jan - Masařík, Michal - Pumera, Martin
PY  - 2023
TI  - Magnetically Driven Self-Degrading Zinc-Containing Cystine Microrobots for Treatment of Prostate Cancer
JF  - SMALL
VL  - 19
IS  - 17
SP  - 1-12
EP  - 1-12
PB  - WILEY-V C H VERLAG GMBH
SN  - 16136810
KW  - cysteine
KW  - magnetic actuation
KW  - micromotors
KW  - nanorobots
KW  - self-propulsion
KW  - tumors
UR  - https://onlinelibrary.wiley.com/doi/10.1002/smll.202208259
N2  - Prostate cancer is the most commonly diagnosed tumor disease in men, and its treatment is still a big challenge in standard oncology therapy. Magnetically actuated microrobots represent the most promising technology in modern nanomedicine, offering the advantage of wireless guidance, effective cell penetration, and non-invasive actuation. Here, new biodegradable magnetically actuated zinc/cystine-based microrobots for in situ treatment of prostate cancer cells are reported. The microrobots are fabricated via metal-ion-mediated self-assembly of the amino acid cystine encapsulating superparamagnetic Fe3O4 nanoparticles (NPs) during the synthesis, which allows their precise manipulation by a rotating magnetic field. Inside the cells, the typical enzymatic reducing environment favors the disassembly of the aminoacidic chemical structure due to the cleavage of cystine disulfide bonds and disruption of non-covalent interactions with the metal ions, as demonstrated by in vitro experiments with reduced nicotinamide adenine dinucleotide (NADH). In this way, the cystine microrobots served for site-specific delivery of Zn2+ ions responsible for tumor cell killing via a "Trojan horse effect". This work presents a new concept of cell internalization exploiting robotic systems' self-degradation, proposing a step forward in non-invasive cancer therapy.
ER  -

USSIA, Martina, Mario URSO, Monika KRATOCHVÍLOVÁ, Jiří NAVRÁTIL, Jan BALVAN, Carmen C. C. MAYORGA-MARTINEZ, Jan VYSKOCIL, Michal MASAŘÍK and Martin PUMERA. Magnetically Driven Self-Degrading Zinc-Containing Cystine Microrobots for Treatment of Prostate Cancer. \textit{SMALL}. WEINHEIM: WILEY-V C H VERLAG GMBH, 2023, vol.~19, No~17, p.~1-12. ISSN~1613-6810. Available from: https://dx.doi.org/10.1002/smll.202208259.

Detailed Information on Publication Record