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@article{2292958,
author = {Hánělová, Klára and Raudenská, Martina and Kratochvílová, Monika and Navrátil, Jiří and Vičar, Tomáš and Bugajová, Mária and Gumulec, Jaromír and Masařík, Michal and Balvan, Jan},
article_location = {LONDON},
article_number = {1},
doi = {http://dx.doi.org/10.1186/s12964-023-01126-z},
keywords = {Extracellular vesicles},
language = {eng},
issn = {1478-811X},
journal = {Cell Communication and Signaling},
title = {Autophagy modulators influence the content of important signalling molecules in PS-positive extracellular vesicles},
url = {https://biosignaling.biomedcentral.com/articles/10.1186/s12964-023-01126-z},
volume = {21},
year = {2023}
}
TY - JOUR
ID - 2292958
AU - Hánělová, Klára - Raudenská, Martina - Kratochvílová, Monika - Navrátil, Jiří - Vičar, Tomáš - Bugajová, Mária - Gumulec, Jaromír - Masařík, Michal - Balvan, Jan
PY - 2023
TI - Autophagy modulators influence the content of important signalling molecules in PS-positive extracellular vesicles
JF - Cell Communication and Signaling
VL - 21
IS - 1
SP - 1-21
EP - 1-21
PB - BMC
SN - 1478811X
KW - Extracellular vesicles
UR - https://biosignaling.biomedcentral.com/articles/10.1186/s12964-023-01126-z
N2 - Extracellular vesicles (EVs) are important mediators of intercellular communication in the tumour microenvironment. Many studies suggest that cancer cells release higher amounts of EVs exposing phosphatidylserine (PS) at the surface. There are lots of interconnections between EVs biogenesis and autophagy machinery. Modulation of autophagy can probably affect not only the quantity of EVs but also their content, which can deeply influence the resulting pro-tumourigenic or anticancer effect of autophagy modulators. In this study, we found that autophagy modulators autophinib, CPD18, EACC, bafilomycin A1 (BAFA1), 3-hydroxychloroquine (HCQ), rapamycin, NVP-BEZ235, Torin1, and starvation significantly alter the composition of the protein content of phosphatidylserine-positive EVs (PS-EVs) produced by cancer cells. The greatest impact had HCQ, BAFA1, CPD18, and starvation. The most abundant proteins in PS-EVs were proteins typical for extracellular exosomes, cytosol, cytoplasm, and cell surface involved in cell adhesion and angiogenesis. PS-EVs protein content involved mitochondrial proteins and signalling molecules such as SQSTM1 and TGFβ1 pro-protein. Interestingly, PS-EVs contained no commonly determined cytokines, such as IL-6, IL-8, GRO-α, MCP-1, RANTES, and GM-CSF, which indicates that secretion of these cytokines is not predominantly mediated through PS-EVs. Nevertheless, the altered protein content of PS-EVs can still participate in the modulation of the fibroblast metabolism and phenotype as p21 was accumulated in fibroblasts influenced by EVs derived from CPD18-treated FaDu cells. The altered protein content of PS-EVs (data are available via ProteomeXchange with identifier PXD037164) also provides information about the cellular compartments and processes that are affected by the applied autophagy modulators.
ER -
HÁNĚLOVÁ, Klára, Martina RAUDENSKÁ, Monika KRATOCHVÍLOVÁ, Jiří NAVRÁTIL, Tomáš VIČAR, Mária BUGAJOVÁ, Jaromír GUMULEC, Michal MASAŘÍK and Jan BALVAN. Autophagy modulators influence the content of important signalling molecules in PS-positive extracellular vesicles. \textit{Cell Communication and Signaling}. LONDON: BMC, 2023, vol.~21, No~1, p.~1-21. ISSN~1478-811X. Available from: https://dx.doi.org/10.1186/s12964-023-01126-z.
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