HÁNĚLOVÁ, Klára, Martina RAUDENSKÁ, Monika KRATOCHVÍLOVÁ, Jiří NAVRÁTIL, Tomáš VIČAR, Mária BUGAJOVÁ, Jaromír GUMULEC, Michal MASAŘÍK and Jan BALVAN. Autophagy modulators influence the content of important signalling molecules in PS-positive extracellular vesicles. Cell Communication and Signaling. LONDON: BMC, 2023, vol. 21, No 1, p. 1-21. ISSN 1478-811X. Available from: https://dx.doi.org/10.1186/s12964-023-01126-z.
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Basic information
Original name Autophagy modulators influence the content of important signalling molecules in PS-positive extracellular vesicles
Authors HÁNĚLOVÁ, Klára (203 Czech Republic, belonging to the institution), Martina RAUDENSKÁ (203 Czech Republic, belonging to the institution), Monika KRATOCHVÍLOVÁ (203 Czech Republic, belonging to the institution), Jiří NAVRÁTIL (203 Czech Republic, belonging to the institution), Tomáš VIČAR (203 Czech Republic, belonging to the institution), Mária BUGAJOVÁ (703 Slovakia, belonging to the institution), Jaromír GUMULEC (203 Czech Republic, belonging to the institution), Michal MASAŘÍK (203 Czech Republic, belonging to the institution) and Jan BALVAN (203 Czech Republic, guarantor, belonging to the institution).
Edition Cell Communication and Signaling, LONDON, BMC, 2023, 1478-811X.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10601 Cell biology
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 8.400 in 2022
RIV identification code RIV/00216224:14110/23:00131018
Organization unit Faculty of Medicine
Doi http://dx.doi.org/10.1186/s12964-023-01126-z
UT WoS 000994734500001
Keywords in English Extracellular vesicles
Tags 14110515, 14110518, rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Tereza Miškechová, učo 341652. Changed: 8/4/2024 10:08.
Abstract
Extracellular vesicles (EVs) are important mediators of intercellular communication in the tumour microenvironment. Many studies suggest that cancer cells release higher amounts of EVs exposing phosphatidylserine (PS) at the surface. There are lots of interconnections between EVs biogenesis and autophagy machinery. Modulation of autophagy can probably affect not only the quantity of EVs but also their content, which can deeply influence the resulting pro-tumourigenic or anticancer effect of autophagy modulators. In this study, we found that autophagy modulators autophinib, CPD18, EACC, bafilomycin A1 (BAFA1), 3-hydroxychloroquine (HCQ), rapamycin, NVP-BEZ235, Torin1, and starvation significantly alter the composition of the protein content of phosphatidylserine-positive EVs (PS-EVs) produced by cancer cells. The greatest impact had HCQ, BAFA1, CPD18, and starvation. The most abundant proteins in PS-EVs were proteins typical for extracellular exosomes, cytosol, cytoplasm, and cell surface involved in cell adhesion and angiogenesis. PS-EVs protein content involved mitochondrial proteins and signalling molecules such as SQSTM1 and TGFβ1 pro-protein. Interestingly, PS-EVs contained no commonly determined cytokines, such as IL-6, IL-8, GRO-α, MCP-1, RANTES, and GM-CSF, which indicates that secretion of these cytokines is not predominantly mediated through PS-EVs. Nevertheless, the altered protein content of PS-EVs can still participate in the modulation of the fibroblast metabolism and phenotype as p21 was accumulated in fibroblasts influenced by EVs derived from CPD18-treated FaDu cells. The altered protein content of PS-EVs (data are available via ProteomeXchange with identifier PXD037164) also provides information about the cellular compartments and processes that are affected by the applied autophagy modulators.
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EF18_046/0015974, research and development projectName: Modernizace České infrastruktury pro integrativní strukturní biologii
GA21-06873S, research and development projectName: Metabolická symbióza mezi nádorovými buňkami a fibroblasty asociovaných s nádorem u nádorů v oblasti hlavy a krku
Investor: Czech Science Foundation
LM2018129, research and development projectName: Národní infrastruktura pro biologické a medicínské zobrazování Czech-BioImaging
Investor: Ministry of Education, Youth and Sports of the CR
LM2018140, research and development projectName: e-Infrastruktura CZ (Acronym: e-INFRA CZ)
Investor: Ministry of Education, Youth and Sports of the CR
LM2023042, research and development projectName: Česká infrastruktura pro integrativní strukturní biologii
Investor: Ministry of Education, Youth and Sports of the CR, CIISB - Czech Infrastructure for Integrative Structural Biology
MUNI/A/1343/2022, interní kód MUName: Zátěže kardiovaskulárního systému od A po Z
Investor: Masaryk University, Loads on the cardiovascular system from A to Z
MUNI/A/1370/2022, interní kód MUName: Patofyziologie vybraných komplexních nemocí od molekulární do systémovou úroveň
Investor: Masaryk University, Pathophysiology of selected complex diseases from molecular to systemic level
NU20J-08-00018, research and development projectName: Exosomální RNA produkovaná fibroblasty asociovanými s nádorem jako relevantní marker v prognóze nádorů hlavy a krku
Investor: Ministry of Health of the CR, Subprogram 2 - junior
NU22-03-00202, research and development projectName: Proteom extracelulárních vezikul - neprozkoumaná oblast mezibuněčné komunikace v mikroprostředí nádorů hlavy a krku
Investor: Ministry of Health of the CR, Subprogram 1 - standard
90242, large research infrastructuresName: CIISB III
90250, large research infrastructuresName: Czech-BioImaging III
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