Sensitivity enhancement of capillary electrophoresis‐frontal
analysis based method for characterization of drug‐protein
interactions using on‐line sample preconcentration

J 2023

Sensitivity enhancement of capillary electrophoresis‐frontal analysis based method for characterization of drug‐protein interactions using on‐line sample preconcentration

BRŽEZICKÁ, Taťána, Zdeněk GLATZ and Lenka KOHÚTOVÁ

Basic information

Original name

Sensitivity enhancement of capillary electrophoresis‐frontal analysis based method for characterization of drug‐protein interactions using on‐line sample preconcentration

Authors

BRŽEZICKÁ, Taťána (203 Czech Republic, belonging to the institution), Zdeněk GLATZ (203 Czech Republic, belonging to the institution) and Lenka KOHÚTOVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Journal of Separation Science, Weinheim, Wiley, 2023, 1615-9306

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.100 in 2022

RIV identification code

RIV/00216224:14310/23:00134152

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1002/jssc.202300152

UT WoS

001017663100001

Keywords in English

binding constants; capillary electrophoresis-frontal analysis; human serum albumin; on-line preconcentration

Abstract

V originále

Capillary electrophoresis-frontal analysis is one of the most frequently used approach for the study of plasma protein-drug interactions as a substantial part of the new drug development. However, the capillary electrophoresis-frontal analysis typically combined with ultraviolet visible detection suffers from insufficient concentration sensitivity particularly for substances with limited solubility and low molar absorption coefficient. The sensitivity problem has been solved in this work by its combination with an on-line sample preconcentration. According the knowledge of the authors this combination has never been used to characterize plasma protein-drug binding. It resulted into a fully automated and versatile methodology for the characterization of binding interactions. Further, the validated method minimalizes the experimental errors due to a reduction in the manipulation of samples. Moreover, employing an on-line preconcentration strategy with capillary electrophoresis-frontal analysis using human serum albumin-salicylic acid as a model system improves the drug concentration sensitivity 17 fold compared to the conventional method. The value of binding constant (1.51 ± 0.63)‧104 L/mol obtained by this new capillary electrophoresis-frontal analysis modification is in agreement with the value (1.13 ± 0.28)‧104 L/mol estimated by conventional variant of capillary electrophoresis-frontal analysis without the preconcentration step, as well as with literature data obtained using different techniques.

Links

GA19-08358S, research and development project

Name: Nové přístupy pro studium afinitních interakcí založené na kapilární elektroforéze

Investor: Czech Science Foundation

MUNI/A/1313/2022, interní kód MU

Name: Podpora biochemického výzkumu v roce 2023

Investor: Masaryk University

Citovat

BRŽEZICKÁ, Taťána, Zdeněk GLATZ and Lenka KOHÚTOVÁ. Sensitivity enhancement of capillary electrophoresis‐frontal analysis based method for characterization of drug‐protein interactions using on‐line sample preconcentration. Journal of Separation Science. Weinheim: Wiley, 2023, vol. 46, No 17, p. 1-8. ISSN 1615-9306. Available from: https://dx.doi.org/10.1002/jssc.202300152.

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   author = {Bržezická, Taťána and Glatz, Zdeněk and Kohútová, Lenka},
   article_location = {Weinheim},
   article_number = {17},
   doi = {http://dx.doi.org/10.1002/jssc.202300152},
   keywords = {binding constants; capillary electrophoresis-frontal analysis; human serum albumin; on-line preconcentration},
   language = {eng},
   issn = {1615-9306},
   journal = {Journal of Separation Science},
   title = {Sensitivity enhancement of capillary electrophoresis‐frontal analysis based method for characterization of drug‐protein interactions using on‐line sample preconcentration},
   url = {https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/full/10.1002/jssc.202300152?campaign=woletoc},
   volume = {46},
   year = {2023}
}

TY  - JOUR
ID  - 2293206
AU  - Bržezická, Taťána - Glatz, Zdeněk - Kohútová, Lenka
PY  - 2023
TI  - Sensitivity enhancement of capillary electrophoresis‐frontal analysis based method for characterization of drug‐protein interactions using on‐line sample preconcentration
JF  - Journal of Separation Science
VL  - 46
IS  - 17
SP  - 1-8
EP  - 1-8
PB  - Wiley
SN  - 16159306
KW  - binding constants
KW  - capillary electrophoresis-frontal analysis
KW  - human serum albumin
KW  - on-line preconcentration
UR  - https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/full/10.1002/jssc.202300152?campaign=woletoc
N2  - Capillary electrophoresis-frontal analysis is one of the most frequently used approach for the study of plasma protein-drug interactions as a substantial part of the new drug development. However, the capillary electrophoresis-frontal analysis typically combined with ultraviolet visible detection suffers from insufficient concentration sensitivity particularly for substances with limited solubility and low molar absorption coefficient. The sensitivity problem has been solved in this work by its combination with an on-line sample preconcentration. According the knowledge of the authors this combination has never been used to characterize plasma protein-drug binding. It resulted into a fully automated and versatile methodology for the characterization of binding interactions. Further, the validated method minimalizes the experimental errors due to a reduction in the manipulation of samples. Moreover, employing an on-line preconcentration strategy with capillary electrophoresis-frontal analysis using human serum albumin-salicylic acid as a model system improves the drug concentration sensitivity 17 fold compared to the conventional method. The value of binding constant (1.51 ± 0.63)‧104 L/mol obtained by this new capillary electrophoresis-frontal analysis modification is in agreement with the value (1.13 ± 0.28)‧104 L/mol estimated by conventional variant of capillary electrophoresis-frontal analysis without the preconcentration step, as well as with literature data obtained using different techniques.
ER  -

BRŽEZICKÁ, Taťána, Zdeněk GLATZ and Lenka KOHÚTOVÁ. Sensitivity enhancement of capillary electrophoresis‐frontal analysis based method for characterization of drug‐protein interactions using on‐line sample preconcentration. \textit{Journal of Separation Science}. Weinheim: Wiley, 2023, vol.~46, No~17, p.~1-8. ISSN~1615-9306. Available from: https://dx.doi.org/10.1002/jssc.202300152.

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