Wnt signaling from Gli1-expressing apical stem/progenitor cells is essential for the coordination of tooth root development

LAV, Rupali, Jan KŘIVÁNEK, Neal ANTHWAL and Abigail S. TUCKER. Wnt signaling from Gli1-expressing apical stem/progenitor cells is essential for the coordination of tooth root development. STEM CELL REPORTS. CAMBRIDGE: CELL PRESS, 2023, vol. 18, No 4, p. 1015-1029. ISSN 2213-6711. Available from: https://dx.doi.org/10.1016/j.stemcr.2023.02.004.

Basic information

• Original name: Wnt signaling from Gli1-expressing apical stem/progenitor cells is essential for the coordination of tooth root development
• Authors: LAV, Rupali, Jan KŘIVÁNEK (203 Czech Republic, belonging to the institution), Neal ANTHWAL and Abigail S. TUCKER (guarantor).
• Edition: STEM CELL REPORTS, CAMBRIDGE, CELL PRESS, 2023, 2213-6711.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10605 Developmental biology
• Country of publisher: United States of America
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 5.900 in 2022
• RIV identification code: RIV/00216224:14110/23:00134157
• Organization unit: Faculty of Medicine
• Doi: http://dx.doi.org/10.1016/j.stemcr.2023.02.004
• UT WoS: 000981833300001
• Keywords in English: stem cells; development; homeostasis; Wnt signaling; tooth development; tooth defects; Hh signaling; Sox9
• Tags: 14110517, rivok
• Tags: International impact, Reviewed

Abstract

Stem cell regulation plays a crucial role during development and homeostasis. Here, an essential source of Wnts from Gli1+ stem/progenitor cells was identified in the murine molar. Loss of Wnt production in Gli1+ apical stem/progenitor cells led to loss of Axin2 at the root apex, mis-regulation of SOX9, loss of BMP and Hh signaling, and truncation of root development. In the absence of Wnt signals, the root epithelium lost its integrity and epithelial identity. This phenotype could be partially mimicked by loss of Sox9 in the Gli1 population. Stabilization of Wnt signaling in the apical papilla led to rapid unordered differentiation of hard tissues and fragmentation of the epithelial root sheath. Wnt signaling from Gli1+ stem/progenitor cells, therefore, orchestrates root development, coordinating mesenchymal and epithelial interactions via SOX9 to regulate stem/progenitor cell expansion and differentiation. Our results demonstrate that disparate stem/progenitor cell populations are unified in their fundamental signaling interactions.

Links

• GA23-06160S, research and development project: Name: Fluktuace mikroprostředí kmenových buněk jako zdroj tkáňové adaptability ve zdraví a nemoci

Investor: Czech Science Foundation, Fluctuation of the stem cell niche as a source of tissue adaptability in health and disease