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@article{2295031,
author = {Ballonová, Lucie and Kulíšková, Petra and Slanina, Peter and Štíchová, Julie and Vlková, Marcela and Hakl, Roman and Litzman, Jiří and Souček, Přemysl and Freiberger, Tomáš},
article_location = {DORDRECHT},
article_number = {6},
doi = {http://dx.doi.org/10.1007/s11033-023-08391-8},
keywords = {PLAUR; uPAR; HAE; Differentiation; Monocyte; Macrophages},
language = {eng},
issn = {0301-4851},
journal = {Molecular Biology Reports},
title = {PLAUR splicing pattern in hereditary angioedema patients' monocytes and macrophages},
url = {https://link.springer.com/article/10.1007/s11033-023-08391-8},
volume = {50},
year = {2023}
}
TY - JOUR
ID - 2295031
AU - Ballonová, Lucie - Kulíšková, Petra - Slanina, Peter - Štíchová, Julie - Vlková, Marcela - Hakl, Roman - Litzman, Jiří - Souček, Přemysl - Freiberger, Tomáš
PY - 2023
TI - PLAUR splicing pattern in hereditary angioedema patients' monocytes and macrophages
JF - Molecular Biology Reports
VL - 50
IS - 6
SP - 4975-4982
EP - 4975-4982
PB - Springer
SN - 03014851
KW - PLAUR
KW - uPAR
KW - HAE
KW - Differentiation
KW - Monocyte
KW - Macrophages
UR - https://link.springer.com/article/10.1007/s11033-023-08391-8
N2 - BackgroundThe PLAUR gene encodes the urokinase-like plasminogen activator receptor (uPAR) and may undergo alternative splicing. Excluding cassette exons 3, 5 and 6 from the transcript results in truncated protein variants whose precise functions have not been elucidated yet. The PLAUR gene is one of several expressed in myeloid cells, where uPAR participates in different cellular processes, including the contact activation system and kallikrein-kinin system, which play an important role in hereditary angioedema (HAE) pathogenesis. A hypothesis about the PLAUR splicing pattern impact on HAE severity was tested.Methods and resultsThe RT-PCR quantified by capillary electrophoresis was used. Although no significant difference in alternative transcript frequency was observed between healthy volunteers and HAE patients, a significant increase in all cassette exon inclusion variants was revealed during monocyte-to-macrophage differentiation.ConclusionsPLAUR alternative splicing in monocytes and macrophages neither was different between HAE patients and healthy controls, nor reflected disease severity. However, the results showed an PLAUR splicing pattern was changing during monocyte-to-macrophage differentiation, but the significance of these changes is unknown and awaits future clarification.
ER -
BALLONOVÁ, Lucie, Petra KULÍŠKOVÁ, Peter SLANINA, Julie ŠTÍCHOVÁ, Marcela VLKOVÁ, Roman HAKL, Jiří LITZMAN, Přemysl SOUČEK and Tomáš FREIBERGER. PLAUR splicing pattern in hereditary angioedema patients' monocytes and macrophages. \textit{Molecular Biology Reports}. DORDRECHT: Springer, 2023, vol.~50, No~6, p.~4975-4982. ISSN~0301-4851. Available from: https://dx.doi.org/10.1007/s11033-023-08391-8.
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