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@article{229551,
author = {Jesenská, Andrea and Sedláček, Ivo and Damborský, Jiří},
article_number = {1},
keywords = {holoalkane dehalogenases},
language = {eng},
issn = {0099-2240},
journal = {Applied and Environmental Microbiology},
title = {Dehalogenation of haloalkanes by Mycobacterium tuberculosis H37Rv and other mycobacteria},
url = {http://www.ncbr.chemi.muni.cz/~jiri/ABSTRACTS/aem00.html},
volume = {66},
year = {2000}
}
TY - JOUR
ID - 229551
AU - Jesenská, Andrea - Sedláček, Ivo - Damborský, Jiří
PY - 2000
TI - Dehalogenation of haloalkanes by Mycobacterium tuberculosis H37Rv and other mycobacteria
JF - Applied and Environmental Microbiology
VL - 66
IS - 1
SP - 219
EP - 219
SN - 00992240
KW - holoalkane dehalogenases
UR - http://www.ncbr.chemi.muni.cz/~jiri/ABSTRACTS/aem00.html
N2 - Haloalkane dehalogenases convert haloalkanes to their corresponding alcohols by a hydrolytic mechanism. To date various haloalkane dehalogenases have been isolated from bacteria colonizing environments that are contaminated with halogenated compounds. A search in current databases with the sequences of these known haloalkane dehalogenases revealed the presence of three different genes encoding putative haloalkane dehalogenases in the genome of the human parasite Mycobacterium tuberculosis strain H37Rv. The ability of M. tuberculosis and several other mycobacterial strains to dehalogenate haloaliphatic compounds was therefore studied. Intact cells of M. tuberculosis H37Rv were found to dehalogenate 1-chlorobutane, 1-chlorodecane, 1-bromobutane and 1,2-dibromoethane. Nine isolates of mycobacteria from clinical material and four strains from a collection of microorganisms were found to be capable of dehalogenating 1,2-dibromoethane. Crude extracts prepared from two of these strains, M. avium MU1 and M. smegmatis CCM 4622, showed broad substrate specificity towards a number of halogenated substrates. Dehalogenase activity in the absence of oxygen and the identification of primary alcohols as the products of the reaction suggest a hydrolytic dehalogenation mechanism. The presence of dehalogenases in bacterial isolates from clinical material including the species colonising both animal tissues and free environment indicates possible role of parasitic microorganisms in distribution of degradation genes in the environment.
ER -
JESENSKÁ, Andrea, Ivo SEDLÁČEK and Jiří DAMBORSKÝ. Dehalogenation of haloalkanes by Mycobacterium tuberculosis H37Rv and other mycobacteria. \textit{Applied and Environmental Microbiology}. 2000, vol.~66, No~1, p.~219-222. ISSN~0099-2240.
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