Association of CDX2 haplogenotype with risk of reflux esophagitis, Barrett´s esophagus, and esophageal adenocarcinoma development

BOŘILOVÁ LINHARTOVÁ, Petra, Natálie MLČŮCHOVÁ, Michaela CVANOVÁ, Zdeněk PAVLOVSKÝ, Miroslava CHOVANCOVÁ, Jiří DOLINA, Lumír KUNOVSKÝ, Radek KROUPA, Lydie IZAKOVIČOVÁ HOLLÁ, Ondřej URBAN, Tomáš HARUŠTIAK and Zdeněk KALA. Association of CDX2 haplogenotype with risk of reflux esophagitis, Barrett´s esophagus, and esophageal adenocarcinoma development. In Cancer World congress, Palermo 2023. 2023.

Basic information

• Original name: Association of CDX2 haplogenotype with risk of reflux esophagitis, Barrett´s esophagus, and esophageal adenocarcinoma development
• Authors: BOŘILOVÁ LINHARTOVÁ, Petra (203 Czech Republic, guarantor, belonging to the institution), Natálie MLČŮCHOVÁ (203 Czech Republic), Michaela CVANOVÁ (203 Czech Republic), Zdeněk PAVLOVSKÝ (203 Czech Republic), Miroslava CHOVANCOVÁ (203 Czech Republic), Jiří DOLINA (203 Czech Republic), Lumír KUNOVSKÝ (203 Czech Republic), Radek KROUPA (203 Czech Republic), Lydie IZAKOVIČOVÁ HOLLÁ (203 Czech Republic), Ondřej URBAN, Tomáš HARUŠTIAK and Zdeněk KALA (203 Czech Republic).
• Edition: Cancer World congress, Palermo 2023, 2023.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10608 Biochemistry and molecular biology
• Country of publisher: Italy
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/23:00131174
• Organization unit: Faculty of Science
• Keywords in English: CDX2; gastroesophageal reflux disease; esophagitis; markers

Abstract

Introduction: Gastroesophageal reflux disease (GERD) can lead to reflux esophagitis (RE), Barrett’s esophagus (BE), and esophageal adenocarcinoma (EAC) development. CDX2 serves as a diagnostic marker for BE and EAC. Objective: Here, we present a genetic association study focused on CDX2 gene variability in Central European patients with GERD. Methods: In 376 endoscopically and histopathologically examined subjects: 279 cases (144 RE, 88 BE, 47 EAC) and 97 controls with nonerosive reflux disease, NERD), genomic DNA was analyzed for 2 polymorphisms in CDX2 (rs3812863, rs4769585). In a subgroup of cases (n=35), CDX2 was immunohistochemically examined in paired pathological and adjacent tissues of esophagus. Results: The distribution of allele frequencies of both CDX2 (rs3812863, rs4769585) polymorphisms differed between cases and controls, the CC genotype (rs4769585) was associated with risk of RE, BE, and EAC development (p≤0.05). The CDX2 AA/CC haplogenotype was found more frequent in cases than in controls (p<0.01). CDX2 protein was found in all BE (n=19) and EAC tissues (n=6), as well as in 3 RE tissues and in 2 esophageal tissue adjacent to lesions (n=35). Conclusion: The CDX2 is a specific tissue marker for BE and EAC diagnosis; variability in the CDX2 gene affects the risk of GERD development.

Links

• LM2018121, research and development project: Name: Výzkumná infrastruktura RECETOX (Acronym: RECETOX RI)

Investor: Ministry of Education, Youth and Sports of the CR, RECETOX RI

• NU20-03-00126, research and development project: Name: Hostitelský mikrobiom ve vztahu k rozvoji Barrettova jícnu a adenokarcinomu jícnu

Investor: Ministry of Health of the CR, Host microbiome in relation to Barrett ́s esophagus and esophageal adenocarcinoma development, Subprogram 1 - standard