Molecular markers for risk stratification of early-and late-onset complications in patients with inhalation injury

VYKLICKÁ, Kateřina, Petra BOŘILOVÁ LINHARTOVÁ and Břetislav LIPOVÝ. Molecular markers for risk stratification of early-and late-onset complications in patients with inhalation injury. In RECETOX PhD Conference 2023. 2023.

Basic information

• Original name: Molecular markers for risk stratification of early-and late-onset complications in patients with inhalation injury
• Authors: VYKLICKÁ, Kateřina (203 Czech Republic, belonging to the institution), Petra BOŘILOVÁ LINHARTOVÁ (203 Czech Republic, guarantor) and Břetislav LIPOVÝ (203 Czech Republic).
• Edition: RECETOX PhD Conference 2023, 2023.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10608 Biochemistry and molecular biology
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/23:00131176
• Organization unit: Faculty of Science
• Keywords in English: inhalation injury; biomarkers; microbiome; antibiotic resistance genes; miRNA; toxicology

Abstract

Inhalation injury (INHI) is defined as an acute airway injury caused by inhalation of hot steam and/or products of combustion. Mortality in patients with INHI is often associated with the development of secondary complications such as extensive infections, pneumonia, direct mucosal damage, mucosal sloughing, and obstructions. Currently, it is acknowledged that the major problem lies in the lack of uniformity in diagnosis, grading, and treatment. The investigation of specific biomarkers for INHI has a great potential to address this issue. Such biomarkers could serve not only to unify diagnosis and treatment, but also to predict the development of secondary complications in patients with INHI. Due to the rarity of the injury, this complex project was designed as a prospective study with the aim of monitoring individual patient parameters during hospitalization. Samples of several matrices (buccal and oropharyngeal swabs, bronchoalveolar lavage - BAL, urine, and blood) for subsequent analyses are collected at 6 time points. In my thesis, I focus on the molecular biological aspects of INHI. Therefore, the main aims are: i) to determine the microbiome and antibiotic resistance genes in samples from upper and lower airways, blood, and/or urine of patients with INHI and with/without infectious complications during their hospitalization; and ii) to select miRNA correlating with INHI using RNAseq analysis in BAL and urine samples, and iii) to characterize toxic compound in BAL and urine.

Links

• EF17_043/0009632, research and development project: Name: CETOCOEN Excellence
• LM2023069, research and development project: Name: Výzkumná infrastruktura RECETOX

Investor: Ministry of Education, Youth and Sports of the CR, RECETOX research infrastructure

• 857560, interní kód MU(CEP code: EF17_043/0009632): Name: CETOCOEN Excellence (Acronym: CETOCOEN Excellence)

Investor: European Union, Spreading excellence and widening participation