Molecular markers for risk stratification of early-and
late-onset complications in patients with inhalation injury

a 2023

Molecular markers for risk stratification of early-and late-onset complications in patients with inhalation injury

VYKLICKÁ, Kateřina, Petra BOŘILOVÁ LINHARTOVÁ and Břetislav LIPOVÝ

Basic information

Original name

Molecular markers for risk stratification of early-and late-onset complications in patients with inhalation injury

Authors

VYKLICKÁ, Kateřina (203 Czech Republic, belonging to the institution), Petra BOŘILOVÁ LINHARTOVÁ (203 Czech Republic, guarantor) and Břetislav LIPOVÝ (203 Czech Republic)

Edition

RECETOX PhD Conference 2023, 2023

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/23:00131176

Organization unit

Faculty of Science

Keywords in English

inhalation injury; biomarkers; microbiome; antibiotic resistance genes; miRNA; toxicology

Změněno: 7/7/2023 11:21, Mgr. Terezie Slámová

Abstract

V originále

Inhalation injury (INHI) is defined as an acute airway injury caused by inhalation of hot steam and/or products of combustion. Mortality in patients with INHI is often associated with the development of secondary complications such as extensive infections, pneumonia, direct mucosal damage, mucosal sloughing, and obstructions. Currently, it is acknowledged that the major problem lies in the lack of uniformity in diagnosis, grading, and treatment. The investigation of specific biomarkers for INHI has a great potential to address this issue. Such biomarkers could serve not only to unify diagnosis and treatment, but also to predict the development of secondary complications in patients with INHI. Due to the rarity of the injury, this complex project was designed as a prospective study with the aim of monitoring individual patient parameters during hospitalization. Samples of several matrices (buccal and oropharyngeal swabs, bronchoalveolar lavage - BAL, urine, and blood) for subsequent analyses are collected at 6 time points. In my thesis, I focus on the molecular biological aspects of INHI. Therefore, the main aims are: i) to determine the microbiome and antibiotic resistance genes in samples from upper and lower airways, blood, and/or urine of patients with INHI and with/without infectious complications during their hospitalization; and ii) to select miRNA correlating with INHI using RNAseq analysis in BAL and urine samples, and iii) to characterize toxic compound in BAL and urine.

Links

EF17_043/0009632, research and development project

Name: CETOCOEN Excellence

LM2023069, research and development project

Name: Výzkumná infrastruktura RECETOX

Investor: Ministry of Education, Youth and Sports of the CR, RECETOX research infrastructure

857560, interní kód MU
(CEP code: EF17_043/0009632)

Name: CETOCOEN Excellence (Acronym: CETOCOEN Excellence)

Investor: European Union, Spreading excellence and widening participation

Citovat

VYKLICKÁ, Kateřina, Petra BOŘILOVÁ LINHARTOVÁ and Břetislav LIPOVÝ. Molecular markers for risk stratification of early-and late-onset complications in patients with inhalation injury. In RECETOX PhD Conference 2023. 2023.

@proceedings{2296511,
   author = {Vyklická, Kateřina and Bořilová Linhartová, Petra and Lipový, Břetislav},
   booktitle = {RECETOX PhD Conference 2023},
   keywords = {inhalation injury; biomarkers; microbiome; antibiotic resistance genes; miRNA; toxicology},
   language = {eng},
   title = {Molecular markers for risk stratification of early-and late-onset complications in patients with inhalation injury},
   year = {2023}
}

TY  - CONF
ID  - 2296511
AU  - Vyklická, Kateřina - Bořilová Linhartová, Petra - Lipový, Břetislav
PY  - 2023
TI  - Molecular markers for risk stratification of early-and late-onset complications in patients with inhalation injury
KW  - inhalation injury
KW  - biomarkers
KW  - microbiome
KW  - antibiotic resistance genes
KW  - miRNA
KW  - toxicology
N2  - Inhalation injury (INHI) is defined as an acute airway injury caused by inhalation of hot steam and/or products of combustion. Mortality in patients with INHI is often associated with the development of secondary complications such as extensive infections, pneumonia, direct mucosal damage, mucosal sloughing, and obstructions. Currently, it is acknowledged that the major problem lies in the lack of uniformity in diagnosis, grading, and treatment. The investigation of specific biomarkers for INHI has a great potential to address this issue. Such biomarkers could serve not only to unify diagnosis and treatment, but also to predict the development of secondary complications in patients with INHI. Due to the rarity of the injury, this complex project was designed as a prospective study with the aim of monitoring individual patient parameters during hospitalization. Samples of several matrices (buccal and oropharyngeal swabs, bronchoalveolar lavage - BAL, urine, and blood) for subsequent analyses are collected at 6 time points. In my thesis, I focus on the molecular biological aspects of INHI. Therefore, the main aims are: i) to determine the microbiome and antibiotic resistance genes in samples from upper and lower airways, blood, and/or urine of patients with INHI and with/without infectious complications during their hospitalization; and ii) to select miRNA correlating with INHI using RNAseq analysis in BAL and urine samples, and iii) to characterize toxic compound in BAL and urine.
ER  -

Detailed Information on Publication Record