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@article{2296857, author = {Straková, Petra and Bednář, Petr and Kotouček, Jan and Holoubek, Jiří and Fořtová, Andrea and Svoboda, Pavel and Štefánik, Michal and Huvarová, Ivana and Šimečková, Pavlína and Mašek, Josef and Gvozdev, Daniil A and Mikhnovets, Igor E and Chistov, Alexey A and Nikitin, Timofei D and Krasilnikov, Maxim S and Ustinov, Alexey V and Alferova, Vera A and Korshun, Vladimir A and Růžek, Daniel and Eyer, Luděk}, article_number = {September}, doi = {http://dx.doi.org/10.1016/j.virusres.2023.199158}, keywords = {SARS-CoV-2; Perylene-related compound; Antiviral activity; Membrane; Liposome; Photodynamic inactivation}, language = {eng}, issn = {0168-1702}, journal = {Virus Research}, title = {Antiviral activity of singlet oxygen-photogenerating perylene compounds against SARS-CoV-2: Interaction with the viral envelope and photodynamic virion inactivation}, url = {https://doi.org/10.1016/j.virusres.2023.199158}, volume = {334}, year = {2023} }
TY - JOUR ID - 2296857 AU - Straková, Petra - Bednář, Petr - Kotouček, Jan - Holoubek, Jiří - Fořtová, Andrea - Svoboda, Pavel - Štefánik, Michal - Huvarová, Ivana - Šimečková, Pavlína - Mašek, Josef - Gvozdev, Daniil A - Mikhnovets, Igor E - Chistov, Alexey A - Nikitin, Timofei D - Krasilnikov, Maxim S - Ustinov, Alexey V - Alferova, Vera A - Korshun, Vladimir A - Růžek, Daniel - Eyer, Luděk PY - 2023 TI - Antiviral activity of singlet oxygen-photogenerating perylene compounds against SARS-CoV-2: Interaction with the viral envelope and photodynamic virion inactivation JF - Virus Research VL - 334 IS - September SP - 1-21 EP - 1-21 PB - Elsevier SN - 01681702 KW - SARS-CoV-2 KW - Perylene-related compound KW - Antiviral activity KW - Membrane KW - Liposome KW - Photodynamic inactivation UR - https://doi.org/10.1016/j.virusres.2023.199158 N2 - The coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has prompted great interest in novel broad-spectrum antivirals, including perylene-related compounds. In the present study, we performed a structure–activity relationship analysis of a series of perylene derivatives, which comprised a large planar perylene residue, and structurally divergent polar groups connected to the perylene core by a rigid ethynyl or thiophene linker. Most of the tested compounds did not exhibit significant cytotoxicity towards multiple cell types susceptible to SARS-CoV-2 infection, and did not change the expressions of cellular stress-related genes under normal light conditions. These compounds showed nanomolar or sub-micromolar dose-dependent anti-SARS-CoV-2 activity, and also suppressed the in vitro replication of feline coronavirus (FCoV), also termed feline infectious peritonitis virus (FIPV). Perylene compounds exhibited high affinity for liposomal and cellular membranes, and efficiently intercalated into the envelopes of SARS-CoV-2 virions, thereby blocking the viral–cell fusion machinery. Furthermore, the studied compounds were demonstrated to be potent photosensitizers, generating reactive oxygen species (ROS), and their anti-SARS-CoV-2 activities were considerably enhanced after irradiation with blue light. Our results indicated that photosensitization is the major mechanism underlying the anti-SARS-CoV-2 activity of perylene derivatives, with these compounds completely losing their antiviral potency under red light. Overall, perylene-based compounds are broad-spectrum antivirals against multiple enveloped viruses, with antiviral action based on light-induced photochemical damage (ROS-mediated, likely singlet oxygen-mediated), causing impairment of viral membrane rheology. ER -
STRAKOVÁ, Petra, Petr BEDNÁŘ, Jan KOTOUČEK, Jiří HOLOUBEK, Andrea FOŘTOVÁ, Pavel SVOBODA, Michal ŠTEFÁNIK, Ivana HUVAROVÁ, Pavlína ŠIMEČKOVÁ, Josef MAŠEK, Daniil A GVOZDEV, Igor E MIKHNOVETS, Alexey A CHISTOV, Timofei D NIKITIN, Maxim S KRASILNIKOV, Alexey V USTINOV, Vera A ALFEROVA, Vladimir A KORSHUN, Daniel RŮŽEK a Luděk EYER. Antiviral activity of singlet oxygen-photogenerating perylene compounds against SARS-CoV-2: Interaction with the viral envelope and photodynamic virion inactivation. \textit{Virus Research}. Elsevier, 2023, roč.~334, September, s.~1-21. ISSN~0168-1702. Dostupné z: https://dx.doi.org/10.1016/j.virusres.2023.199158.
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