A novel heteroleptic Cu(II)-phenanthroline-UDCA complex as
lipoxygenase inhibitor and ER-stress inducer in cancer cell
lines

J 2023

A novel heteroleptic Cu(II)-phenanthroline-UDCA complex as lipoxygenase inhibitor and ER-stress inducer in cancer cell lines

MASURI, Sebastiano, Lukáš MORÁŇ, Tereza VESSELÁ, Enzo CADONI, Maria Grazia CABIDDU et. al.

Basic information

Original name

A novel heteroleptic Cu(II)-phenanthroline-UDCA complex as lipoxygenase inhibitor and ER-stress inducer in cancer cell lines

Authors

MASURI, Sebastiano, Lukáš MORÁŇ (203 Czech Republic, belonging to the institution), Tereza VESSELÁ (203 Czech Republic, belonging to the institution), Enzo CADONI, Maria Grazia CABIDDU, Lukáš PEČINKA (203 Czech Republic, belonging to the institution), Viktorie GABRIELOVÁ (203 Czech Republic, belonging to the institution), Francesca MELONI, Josef HAVEL (203 Czech Republic, belonging to the institution), Petr VAŇHARA (203 Czech Republic, belonging to the institution) and Tiziana PIVETTA (guarantor)

Edition

Journal of Inorganic Biochemistry, New York, Elsevier, 2023, 0162-0134

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.900 in 2022

RIV identification code

RIV/00216224:14110/23:00131184

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.jinorgbio.2023.112301

UT WoS

001032239600001

Keywords in English

Copper complex; Solution equilibria; Mass spectrometry; Lipoxygenase; Molecular docking; ER stress

Abstract

V originále

A new heteroleptic copper(II) compound named C0-UDCA was prepared by reaction of [Cu(phen)2(OH2)](ClO4)2 (C0) with the bile ursodeoxycholic acid (UDCA). The resulting compound is able to inhibit the lipoxygenase enzyme showing more efficacy than the precursors C0 and UDCA. Molecular docking simulations clarified the interactions with the enzyme as due to allosteric modulation. The new complex shows antitumoral effect on ovarian (SKOV-3) and pancreatic (PANC-1) cancer cells at the Endoplasmic Reticulum (ER) level by activating the Unfolded Protein Response. In particular, the chaperone BiP, the pro-apoptotic protein CHOP and the transcription factor ATF6 are upregulated in the presence of C0-UDCA. The combination of Intact Cell MALDI-MS and statistical analysis have allowed us to discriminate between untreated and treated cells based on their mass spectrometry fingerprints.

Links

MUNI/A/1298/2022, interní kód MU

Name: Základní a aplikovaný výzkum a vývoj metod chemické a fyzikálně chemické analýzy pro studium přírody a pokročilé technologie

Investor: Masaryk University, Basic and applied research and development of chemical and physicochemical analytical methods for the study of nature and advanced technology

MUNI/A/1301/2022, interní kód MU

Name: Zdroje pro tkáňové inženýrství 13

Investor: Masaryk University

MUNI/11/ACC/3/2022, interní kód MU

Name: Bioanalytical quality control of cGMP/ATMP-grade stem cells and progenitors

Investor: Masaryk University, Accelerate

NU23-08-00241, research and development project

Name: Vývoj ex-vivo buněčných modelů pro adenokarcinom pankreatu: markery a cíle pro precizní medicínu

Investor: Ministry of Health of the CR, Development of ex vivo cellular models for pancreatic adenocarcinoma: markers and targets for precision medicine, Subprogram 1 - standard

ROZV/28/LF/2020, interní kód MU

Name: LF - Příspěvek na InstP 2020

Investor: Ministry of Education, Youth and Sports of the CR, Internal development projects

Citovat

MASURI, Sebastiano, Lukáš MORÁŇ, Tereza VESSELÁ, Enzo CADONI, Maria Grazia CABIDDU, Lukáš PEČINKA, Viktorie GABRIELOVÁ, Francesca MELONI, Josef HAVEL, Petr VAŇHARA and Tiziana PIVETTA. A novel heteroleptic Cu(II)-phenanthroline-UDCA complex as lipoxygenase inhibitor and ER-stress inducer in cancer cell lines. Journal of Inorganic Biochemistry. New York: Elsevier, 2023, vol. 246, September 2023, p. 1-14. ISSN 0162-0134. Available from: https://dx.doi.org/10.1016/j.jinorgbio.2023.112301.

@article{2296867,
   author = {Masuri, Sebastiano and Moráň, Lukáš and Vesselá, Tereza and Cadoni, Enzo and Cabiddu, Maria Grazia and Pečinka, Lukáš and Gabrielová, Viktorie and Meloni, Francesca and Havel, Josef and Vaňhara, Petr and Pivetta, Tiziana},
   article_location = {New York},
   article_number = {September 2023},
   doi = {http://dx.doi.org/10.1016/j.jinorgbio.2023.112301},
   keywords = {Copper complex; Solution equilibria; Mass spectrometry; Lipoxygenase; Molecular docking; ER stress},
   language = {eng},
   issn = {0162-0134},
   journal = {Journal of Inorganic Biochemistry},
   title = {A novel heteroleptic Cu(II)-phenanthroline-UDCA complex as lipoxygenase inhibitor and ER-stress inducer in cancer cell lines},
   url = {https://doi.org/10.1016/j.jinorgbio.2023.112301},
   volume = {246},
   year = {2023}
}

TY  - JOUR
ID  - 2296867
AU  - Masuri, Sebastiano - Moráň, Lukáš - Vesselá, Tereza - Cadoni, Enzo - Cabiddu, Maria Grazia - Pečinka, Lukáš - Gabrielová, Viktorie - Meloni, Francesca - Havel, Josef - Vaňhara, Petr - Pivetta, Tiziana
PY  - 2023
TI  - A novel heteroleptic Cu(II)-phenanthroline-UDCA complex as lipoxygenase inhibitor and ER-stress inducer in cancer cell lines
JF  - Journal of Inorganic Biochemistry
VL  - 246
IS  - September 2023
SP  - 1-14
EP  - 1-14
PB  - Elsevier
SN  - 01620134
KW  - Copper complex
KW  - Solution equilibria
KW  - Mass spectrometry
KW  - Lipoxygenase
KW  - Molecular docking
KW  - ER stress
UR  - https://doi.org/10.1016/j.jinorgbio.2023.112301
N2  - A new heteroleptic copper(II) compound named C0-UDCA was prepared by reaction of [Cu(phen)2(OH2)](ClO4)2 (C0) with the bile ursodeoxycholic acid (UDCA). The resulting compound is able to inhibit the lipoxygenase enzyme showing more efficacy than the precursors C0 and UDCA. Molecular docking simulations clarified the interactions with the enzyme as due to allosteric modulation. The new complex shows antitumoral effect on ovarian (SKOV-3) and pancreatic (PANC-1) cancer cells at the Endoplasmic Reticulum (ER) level by activating the Unfolded Protein Response. In particular, the chaperone BiP, the pro-apoptotic protein CHOP and the transcription factor ATF6 are upregulated in the presence of C0-UDCA. The combination of Intact Cell MALDI-MS and statistical analysis have allowed us to discriminate between untreated and treated cells based on their mass spectrometry fingerprints.
ER  -

MASURI, Sebastiano, Lukáš MORÁŇ, Tereza VESSELÁ, Enzo CADONI, Maria Grazia CABIDDU, Lukáš PEČINKA, Viktorie GABRIELOVÁ, Francesca MELONI, Josef HAVEL, Petr VAŇHARA and Tiziana PIVETTA. A novel heteroleptic Cu(II)-phenanthroline-UDCA complex as lipoxygenase inhibitor and ER-stress inducer in cancer cell lines. \textit{Journal of Inorganic Biochemistry}. New York: Elsevier, 2023, vol.~246, September 2023, p.~1-14. ISSN~0162-0134. Available from: https://dx.doi.org/10.1016/j.jinorgbio.2023.112301.

Detailed Information on Publication Record