| KOBAYASHI, Shintaro, Yukine FUKUDA, Kentaro YOSHII, Passawat THAMMAHAKIN, Keisuke MAEZONO, Luděk EYER, Daniel RŮŽEK and Hiroaki KARIWA. Development of recombinant West Nile virus expressing mCherry reporter protein. Journal of Virological Methods. Amsterdam: Elsevier, 2023, vol. 317, July, p. 1-5. ISSN 0166-0934. Available from: https://dx.doi.org/10.1016/j.jviromet.2023.114744. |
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@article{2296941,
author = {Kobayashi, Shintaro and Fukuda, Yukine and Yoshii, Kentaro and Thammahakin, Passawat and Maezono, Keisuke and Eyer, Luděk and Růžek, Daniel and Kariwa, Hiroaki},
article_location = {Amsterdam},
article_number = {July},
doi = {http://dx.doi.org/10.1016/j.jviromet.2023.114744},
keywords = {West Nile virus; Reporter protein; Production of reporter virus},
language = {eng},
issn = {0166-0934},
journal = {Journal of Virological Methods},
title = {Development of recombinant West Nile virus expressing mCherry reporter protein.},
url = {https://doi.org/10.1016/j.jviromet.2023.114744},
volume = {317},
year = {2023}
}
TY - JOUR
ID - 2296941
AU - Kobayashi, Shintaro - Fukuda, Yukine - Yoshii, Kentaro - Thammahakin, Passawat - Maezono, Keisuke - Eyer, Luděk - Růžek, Daniel - Kariwa, Hiroaki
PY - 2023
TI - Development of recombinant West Nile virus expressing mCherry reporter protein.
JF - Journal of Virological Methods
VL - 317
IS - July
SP - 1-5
EP - 1-5
PB - Elsevier
SN - 01660934
KW - West Nile virus
KW - Reporter protein
KW - Production of reporter virus
UR - https://doi.org/10.1016/j.jviromet.2023.114744
N2 - West Nile virus (WNV) is transmitted to humans and animals by a mosquito and enters the central nervous system, leading to lethal encephalitis. Reporter viruses expressing fluorescent proteins enable detection of infected cells in vitro and in vivo, facilitating evaluation of the dynamics of viral infection, and the development of diagnostic or therapeutic methods. In this study, we developed a method for production of a recombinant replication-competent WNV expressing mCherry fluorescent protein. The expression of mCherry was observed in viral antigen-positive cells in vitro and in vivo, but the growth of the reporter WNV was reduced as compared to the parental WNV. The expression of mCherry was stable during 5 passages in reporter WNV-infected culture cells. Neurological symptoms were observed in mice inoculated intracranially with the reporter WNV. The reporter WNV expressing mCherry will facilitate research into WNV replication in mouse brains.
ER -
KOBAYASHI, Shintaro, Yukine FUKUDA, Kentaro YOSHII, Passawat THAMMAHAKIN, Keisuke MAEZONO, Luděk EYER, Daniel RŮŽEK and Hiroaki KARIWA. Development of recombinant West Nile virus expressing mCherry reporter protein. \textit{Journal of Virological Methods}. Amsterdam: Elsevier, 2023, vol.~317, July, p.~1-5. ISSN~0166-0934. Available from: https://dx.doi.org/10.1016/j.jviromet.2023.114744.
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