Animal model of mucosal inflammation (gastritis) and its
application in balneology

a 2023

Animal model of mucosal inflammation (gastritis) and its application in balneology

PÍREK, Ondřej, Natálie RAFČÍKOVÁ, Xenie BUDÍNSKÁ, Jaroslav NÁDENÍČEK, Michal HENDRYCH et. al.

Basic information

Original name

Animal model of mucosal inflammation (gastritis) and its application in balneology

Name in Czech

Animální model zánětu střevní sliznice (gastritidy) a jeho využití v balneologii

Authors

Edition

31st Meeting of the European Society of Paediatric Clinical Research (ESPCR 2023), 2023

Other information

Language

English

Type of outcome

Konferenční abstrakt

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Odkaz na sborník abstraktů z konference

Organization unit

Faculty of Medicine

Keywords (in Czech)

minerální voda Vincentka, gastritida, animální model, oxidativní poškození

Keywords in English

mineral water Vincentka, gastritis, animal model, oxidative damage

Změněno: 11/7/2023 23:31, MUDr. Ondřej Pírek

Abstract

V originále

Inflammatory diseases of the mucous membrane located in various organs of our body, such as the gastrointestinal tract or respiratory system, are common diseases of children and adults. Since the end of the 17th century, the positive effects of natural mineral water Vincentka from Luhačovice Spa (Czech Republic) on inflamatory diseases have been empirically known. The aim of this pilot study is to verify on an animal model of gastritis - as an example of mucosal inflammation - empirically reported improvement in health during drinking therapy of Vincentka natural mineral water. Sixteen Wistar laboratory rats (one-month-old males, weight 240-280 g) used in the experiment were divided into two groups. Control group of animals (Co) received standard water at a daily dose of 0.9 ml/100 g of their current body weight through an orogastric tube for the first 7 days, and the other group of animals (Vi) received the same dose of Vincentka instead of water. Before starting the application of liquids, an adaptation phase (handling) took place in all animals for 1 week. On the eighth day, gastric inflammation was induced in both groups of animals using indomethacin solution at a dose 1 ml/100 g. Twenty-four hours after the administration of indomethacin, the experiment was terminated. Blood was collected by intracardiac puncture, accompanied by dissection and removal of the stomach and duodenum. The collected tissues and blood were further examined by an immunohistochemical and biochemical methods. The experiment was approved by Ministry of Education, Youth and Sports MSMT-35703/2019-2. We found significantly higher values of the parameters of the antioxidant capacity of the blood plasma in the Vi versus Co group: concentration of the sulfanyl group 225.5 (219.9-272.0) vs. 184.6 (108.4-234.8 µmol/L), p<0.05; 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid 0.104 (0.105-0.815) vs. 0.103 (0.102-0.104 (mgGA/ml)), p<0.05. Histological microscopy showed a reduced number of peptic erosions and ulcerations of the stomach in group Vi (p<0.01). We can assume that "Vincentka mineral water from a natural healing source" is probably the cause protecting the GIT mucosa from the effect of acute inflammation, increases the antioxidant capacity of the body and also reduces signs of oxidative stress.

Links

MUNI/A/1343/2022, interní kód MU

Name: Zátěže kardiovaskulárního systému od A po Z

Investor: Masaryk University, Loads on the cardiovascular system from A to Z

Citovat

PÍREK, Ondřej, Natálie RAFČÍKOVÁ, Xenie BUDÍNSKÁ, Jaroslav NÁDENÍČEK, Michal HENDRYCH, Petr BABULA, Tibor STRAČINA, Marie NOVÁKOVÁ, Magdaléna ŠUDÁKOVÁ, Hana HRSTKOVÁ and Zuzana NOVÁKOVÁ. Animal model of mucosal inflammation (gastritis) and its application in balneology. In 31st Meeting of the European Society of Paediatric Clinical Research (ESPCR 2023). 2023.

@proceedings{2297057,
   author = {Pírek, Ondřej and Rafčíková, Natálie and Budínská, Xenie and Nádeníček, Jaroslav and Hendrych, Michal and Babula, Petr and Stračina, Tibor and Nováková, Marie and Šudáková, Magdaléna and Hrstková, Hana and Nováková, Zuzana},
   booktitle = {31st Meeting of the European Society of Paediatric Clinical Research (ESPCR 2023)},
   keywords = {mineral water Vincentka, gastritis, animal model, oxidative damage},
   language = {eng},
   title = {Animal model of mucosal inflammation (gastritis) and its application in balneology},
   url = {https://espcr2023.hu/programme/ESPCR_abstracts.pdf},
   year = {2023}
}

TY  - CONF
ID  - 2297057
AU  - Pírek, Ondřej - Rafčíková, Natálie - Budínská, Xenie - Nádeníček, Jaroslav - Hendrych, Michal - Babula, Petr - Stračina, Tibor - Nováková, Marie - Šudáková, Magdaléna - Hrstková, Hana - Nováková, Zuzana
PY  - 2023
TI  - Animal model of mucosal inflammation (gastritis) and its application in balneology
KW  - mineral water Vincentka, gastritis, animal model, oxidative damage
UR  - https://espcr2023.hu/programme/ESPCR_abstracts.pdf
N2  - Inflammatory diseases of the mucous membrane located in various organs of our body, such as the gastrointestinal tract or respiratory system, are common diseases of children and adults. Since the end of the 17th century, the positive effects of natural mineral water Vincentka from Luhačovice Spa (Czech Republic) on inflamatory diseases have been empirically known. The aim of this pilot study is to verify on an animal model of gastritis - as an example of mucosal inflammation - empirically reported improvement in health during drinking therapy of Vincentka natural mineral water. Sixteen Wistar laboratory rats (one-month-old males, weight 240-280 g) used in the experiment were divided into two groups. Control group of animals (Co) received standard water at a daily dose of 0.9 ml/100 g of their current body weight through an orogastric tube for the first 7 days, and the other group of animals (Vi) received the same dose of Vincentka instead of water. Before starting the application of liquids, an adaptation phase (handling) took place in all animals for 1 week. On the eighth day, gastric inflammation was induced in both groups of animals using indomethacin solution at a dose 1 ml/100 g. Twenty-four hours after the administration of indomethacin, the experiment was terminated. Blood was collected by intracardiac puncture, accompanied by dissection and removal of the stomach and duodenum. The collected tissues and blood were further examined by an immunohistochemical and biochemical methods. The experiment was approved by Ministry of Education, Youth and Sports MSMT-35703/2019-2. We found significantly higher values of the parameters of the antioxidant capacity of the blood plasma in the Vi versus Co group: concentration of the sulfanyl group 225.5 (219.9-272.0) vs. 184.6 (108.4-234.8 µmol/L), p<0.05; 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid 0.104 (0.105-0.815) vs. 0.103 (0.102-0.104 (mgGA/ml)), p<0.05. Histological microscopy showed a reduced number of peptic erosions and ulcerations of the stomach in group Vi (p<0.01). We can assume that "Vincentka mineral water from a natural healing source" is probably the cause protecting the GIT mucosa from the effect of acute inflammation, increases the antioxidant capacity of the body and also reduces signs of oxidative stress.
ER  -

PÍREK, Ondřej, Natálie RAFČÍKOVÁ, Xenie BUDÍNSKÁ, Jaroslav NÁDENÍČEK, Michal HENDRYCH, Petr BABULA, Tibor STRAČINA, Marie NOVÁKOVÁ, Magdaléna ŠUDÁKOVÁ, Hana HRSTKOVÁ and Zuzana NOVÁKOVÁ. Animal model of mucosal inflammation (gastritis) and its application in balneology. In \textit{31st Meeting of the European Society of Paediatric Clinical Research (ESPCR 2023)}. 2023.

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