| BUCHTELOVÁ, Martina, Lucie BLAHOVÁ, David NEČAS, Petra KŘÍŽKOVÁ, Jana BARTOŠÍKOVÁ, Jiřina MEDALOVÁ, Zdeňka KOLSKÁ, Dirk HEGEMANN and Lenka ZAJÍČKOVÁ. Insight into peculiar adhesion of cells to plasma-chemically prepared multifunctional “amino-glue” surfaces. Plasma Processes and Polymers. Wiley‐VCH GmbH, 2023, vol. 20, No 6, p. 1-15. ISSN 1612-8850. Available from: https://dx.doi.org/10.1002/ppap.202200157. |
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@article{2297299,
author = {Buchtelová, Martina and Blahová, Lucie and Nečas, David and Křížková, Petra and Bartošíková, Jana and Medalová, Jiřina and Kolská, Zdeňka and Hegemann, Dirk and Zajíčková, Lenka},
article_number = {6},
doi = {http://dx.doi.org/10.1002/ppap.202200157},
keywords = {amine plasma polymer; carboxyl plasma polymer; cell adhesion; glycocalyx; nonspecificadhesion},
language = {eng},
issn = {1612-8850},
journal = {Plasma Processes and Polymers},
title = {Insight into peculiar adhesion of cells to plasma-chemically prepared multifunctional “amino-glue” surfaces},
url = {https://onlinelibrary.wiley.com/doi/10.1002/ppap.202200157},
volume = {20},
year = {2023}
}
TY - JOUR
ID - 2297299
AU - Buchtelová, Martina - Blahová, Lucie - Nečas, David - Křížková, Petra - Bartošíková, Jana - Medalová, Jiřina - Kolská, Zdeňka - Hegemann, Dirk - Zajíčková, Lenka
PY - 2023
TI - Insight into peculiar adhesion of cells to plasma-chemically prepared multifunctional “amino-glue” surfaces
JF - Plasma Processes and Polymers
VL - 20
IS - 6
SP - 1-15
EP - 1-15
PB - Wiley‐VCH GmbH
SN - 16128850
KW - amine plasma polymer
KW - carboxyl plasma polymer
KW - cell adhesion
KW - glycocalyx
KW - nonspecificadhesion
UR - https://onlinelibrary.wiley.com/doi/10.1002/ppap.202200157
N2 - Plasma polymers (PPs) can easily modify material surfaces to improve their bio-applicability due to match-made surface-free energy and functionality. However, cell adhesion to PPs typically composed of various functional groups has not yet been fully understood. We explain the origin of strong resistance to trypsin treatment previously noted for nonendothelial cells on amine PPs. It is caused mainly by nonspecific adhesion of negatively charged parts of transmembrane proteins to the positively charged amine PP surface, enabled by thin glycocalyx. However, endothelial cells are bound primarily by their thick, negatively charged glycocalyx and sporadically by integrins in kinetic traps, both cleaved by trypsin. Cell scratching by atomic force microscopy tip confirmed the correlation of trypsin resistance to the strength of cell adhesion.
ER -
BUCHTELOVÁ, Martina, Lucie BLAHOVÁ, David NEČAS, Petra KŘÍŽKOVÁ, Jana BARTOŠÍKOVÁ, Jiřina MEDALOVÁ, Zdeňka KOLSKÁ, Dirk HEGEMANN and Lenka ZAJÍČKOVÁ. Insight into peculiar adhesion of cells to plasma-chemically prepared multifunctional “amino-glue” surfaces. \textit{Plasma Processes and Polymers}. Wiley‐VCH GmbH, 2023, vol.~20, No~6, p.~1-15. ISSN~1612-8850. Available from: https://dx.doi.org/10.1002/ppap.202200157.
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