Insight into peculiar adhesion of cells to plasma-chemically
prepared multifunctional “amino-glue” surfaces

J 2023

Insight into peculiar adhesion of cells to plasma-chemically prepared multifunctional “amino-glue” surfaces

BUCHTELOVÁ, Martina, Lucie BLAHOVÁ, David NEČAS, Petra KŘÍŽKOVÁ, Jana BARTOŠÍKOVÁ et. al.

Basic information

Original name

Insight into peculiar adhesion of cells to plasma-chemically prepared multifunctional “amino-glue” surfaces

Authors

BUCHTELOVÁ, Martina, Lucie BLAHOVÁ, David NEČAS, Petra KŘÍŽKOVÁ (203 Czech Republic, belonging to the institution), Jana BARTOŠÍKOVÁ (203 Czech Republic, belonging to the institution), Jiřina MEDALOVÁ (203 Czech Republic, belonging to the institution), Zdeňka KOLSKÁ, Dirk HEGEMANN and Lenka ZAJÍČKOVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Plasma Processes and Polymers, Wiley‐VCH GmbH, 2023, 1612-8850

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

20506 Coating and films

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.500 in 2022

RIV identification code

RIV/00216224:14310/23:00131232

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1002/ppap.202200157

UT WoS

000941082700001

Keywords in English

amine plasma polymer; carboxyl plasma polymer; cell adhesion; glycocalyx; nonspecificadhesion

Abstract

V originále

Plasma polymers (PPs) can easily modify material surfaces to improve their bio-applicability due to match-made surface-free energy and functionality. However, cell adhesion to PPs typically composed of various functional groups has not yet been fully understood. We explain the origin of strong resistance to trypsin treatment previously noted for nonendothelial cells on amine PPs. It is caused mainly by nonspecific adhesion of negatively charged parts of transmembrane proteins to the positively charged amine PP surface, enabled by thin glycocalyx. However, endothelial cells are bound primarily by their thick, negatively charged glycocalyx and sporadically by integrins in kinetic traps, both cleaved by trypsin. Cell scratching by atomic force microscopy tip confirmed the correlation of trypsin resistance to the strength of cell adhesion.

Links

LM2018127, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

90110, large research infrastructures

Name: CzechNanoLab

90242, large research infrastructures

Name: CIISB III

Citovat

BUCHTELOVÁ, Martina, Lucie BLAHOVÁ, David NEČAS, Petra KŘÍŽKOVÁ, Jana BARTOŠÍKOVÁ, Jiřina MEDALOVÁ, Zdeňka KOLSKÁ, Dirk HEGEMANN and Lenka ZAJÍČKOVÁ. Insight into peculiar adhesion of cells to plasma-chemically prepared multifunctional “amino-glue” surfaces. Plasma Processes and Polymers. Wiley‐VCH GmbH, 2023, vol. 20, No 6, p. 1-15. ISSN 1612-8850. Available from: https://dx.doi.org/10.1002/ppap.202200157.

@article{2297299,
   author = {Buchtelová, Martina and Blahová, Lucie and Nečas, David and Křížková, Petra and Bartošíková, Jana and Medalová, Jiřina and Kolská, Zdeňka and Hegemann, Dirk and Zajíčková, Lenka},
   article_number = {6},
   doi = {http://dx.doi.org/10.1002/ppap.202200157},
   keywords = {amine plasma polymer; carboxyl plasma polymer; cell adhesion; glycocalyx; nonspecificadhesion},
   language = {eng},
   issn = {1612-8850},
   journal = {Plasma Processes and Polymers},
   title = {Insight into peculiar adhesion of cells to plasma-chemically prepared multifunctional “amino-glue” surfaces},
   url = {https://onlinelibrary.wiley.com/doi/10.1002/ppap.202200157},
   volume = {20},
   year = {2023}
}

TY  - JOUR
ID  - 2297299
AU  - Buchtelová, Martina - Blahová, Lucie - Nečas, David - Křížková, Petra - Bartošíková, Jana - Medalová, Jiřina - Kolská, Zdeňka - Hegemann, Dirk - Zajíčková, Lenka
PY  - 2023
TI  - Insight into peculiar adhesion of cells to plasma-chemically prepared multifunctional “amino-glue” surfaces
JF  - Plasma Processes and Polymers
VL  - 20
IS  - 6
SP  - 1-15
EP  - 1-15
PB  - Wiley‐VCH GmbH
SN  - 16128850
KW  - amine plasma polymer
KW  - carboxyl plasma polymer
KW  - cell adhesion
KW  - glycocalyx
KW  - nonspecificadhesion
UR  - https://onlinelibrary.wiley.com/doi/10.1002/ppap.202200157
N2  - Plasma polymers (PPs) can easily modify material surfaces to improve their bio-applicability due to match-made surface-free energy and functionality. However, cell adhesion to PPs typically composed of various functional groups has not yet been fully understood. We explain the origin of strong resistance to trypsin treatment previously noted for nonendothelial cells on amine PPs. It is caused mainly by nonspecific adhesion of negatively charged parts of transmembrane proteins to the positively charged amine PP surface, enabled by thin glycocalyx. However, endothelial cells are bound primarily by their thick, negatively charged glycocalyx and sporadically by integrins in kinetic traps, both cleaved by trypsin. Cell scratching by atomic force microscopy tip confirmed the correlation of trypsin resistance to the strength of cell adhesion.
ER  -

BUCHTELOVÁ, Martina, Lucie BLAHOVÁ, David NEČAS, Petra KŘÍŽKOVÁ, Jana BARTOŠÍKOVÁ, Jiřina MEDALOVÁ, Zdeňka KOLSKÁ, Dirk HEGEMANN and Lenka ZAJÍČKOVÁ. Insight into peculiar adhesion of cells to plasma-chemically prepared multifunctional “amino-glue” surfaces. \textit{Plasma Processes and Polymers}. Wiley‐VCH GmbH, 2023, vol.~20, No~6, p.~1-15. ISSN~1612-8850. Available from: https://dx.doi.org/10.1002/ppap.202200157.

Detailed Information on Publication Record