Detailed Information on Publication Record
2023
Aspergillus niger as a cell factory for the production of pyomelanin, a molecule with UV-C radiation shielding activity
MARIE, Koch Stella, Carsten FREIDANK-POHL, Siontas OLIVER, Cortesao MARTA, Mota AFONSO et. al.Basic information
Original name
Aspergillus niger as a cell factory for the production of pyomelanin, a molecule with UV-C radiation shielding activity
Authors
MARIE, Koch Stella, Carsten FREIDANK-POHL, Siontas OLIVER, Cortesao MARTA, Mota AFONSO, Runzheimer KATHARINA, Jung SASCHA, Katarína REBROŠOVÁ (703 Slovakia, belonging to the institution), Martin SILER, Moeller RALF and Meyer VERA
Edition
FRONTIERS IN MICROBIOLOGY, LAUSANNE, FRONTIERS MEDIA SA, 2023, 1664-302X
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10606 Microbiology
Country of publisher
Switzerland
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 5.200 in 2022
RIV identification code
RIV/00216224:14110/23:00131292
Organization unit
Faculty of Medicine
UT WoS
001041737100001
Keywords in English
Aspergillus niger; melanin; pyomelanin; radioprotection; cosmic radiation; space exploration
Tags
International impact, Reviewed
Změněno: 26/1/2024 08:10, Mgr. Tereza Miškechová
Abstract
V originále
Melanins are complex pigments with various biological functions and potential applications in space exploration and biomedicine due to their radioprotective properties. Aspergillus niger, a fungus known for its high radiation resistance, is widely used in biotechnology and a candidate for melanin production. In this study, we investigated the production of fungal pyomelanin (PyoFun) in A. niger by inducing overproduction of the pigment using L-tyrosine in a recombinant ΔhmgA mutant strain (OS4.3). The PyoFun pigment was characterized using three spectroscopic methods, and its antioxidant properties were assessed using a DPPH-assay. Additionally, we evaluated the protective effect of PyoFun against non-ionizing radiation (monochromatic UV-C) and compared its efficacy to a synthetically produced control pyomelanin (PyoSyn). The results confirmed successful production of PyoFun in A. niger through inducible overproduction. Characterization using spectroscopic methods confirmed the presence of PyoFun, and the DPPH-assay demonstrated its strong antioxidant properties. Moreover, PyoFun exhibited a highly protective effect against radiation-induced stress, surpassing the protection provided by PyoSyn. The findings of this study suggest that PyoFun has significant potential as a biological shield against harmful radiation. Notably, PyoFun is synthesized extracellularly, differing it from other fungal melanins (such as L-DOPA- or DHN-melanin) that require cell lysis for pigment purification. This characteristic makes PyoFun a valuable resource for biotechnology, biomedicine, and the space industry. However, further research is needed to evaluate its protective effect in a dried form and against ionizing radiation.
Links
MUNI/A/1361/2022, interní kód MU |
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NU21-05-00341, research and development project |
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