Correlation between Zn/Cu ratio and Bisphenol A in seminal
plasma in men with varicocele.

JEŠETA, Michal, Jana NAVRÁTILOVÁ, Jiří KALINA, Lenka MEKIŇOVÁ, Jana ŽÁKOVÁ, Pavel VENTRUBA and Igor CRHA. Correlation between Zn/Cu ratio and Bisphenol A in seminal plasma in men with varicocele. (Correlation between Zn/Cu ratio and Bisphenol A in seminal plasma in men with varicocele). In 38th Hybrid Annual Meeting of the ESHRE. 2022. ISSN 0268-1161.

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TY  - CONF
ID  - 2299245
AU  - Ješeta, Michal - Navrátilová, Jana - Kalina, Jiří - Mekiňová, Lenka - Žáková, Jana - Ventruba, Pavel - Crha, Igor
PY  - 2022
TI  - Correlation between Zn/Cu ratio and Bisphenol A in seminal plasma in men with varicocele.
KW  - Bisphenol A
KW  - spermiogram parameters
KW  - varicocele
UR  - https://academic.oup.com/humrep/article/37/Supplement_1/deac107.063/6620630
N2  - Is there a relationship between concentration of Bisphenol A (BPA), Zn contents and Zn/Cu ratio in seminal fluid and spermiogram parameters in men with varicocele? Our results document correlations between BPA, Zn/Cu and sperm motility. We found that the negative effect of BPA can be attenuated by higher Zn/Cu levels. A range of environmental factors including organic endocrine disruptors affects spermatogenesis. Bisphenols are endocrine disruptors often used in production of plastics. BPA is an endocrine disruptor with negative effects on reproduction. Presence of BPA also induces production of reactive oxygen species with negative impact on spermatogenesis. Zinc and copper are trace elements important for human spermatogenesis. Zn and especially the ratio of Zn/Cu are good parameters of the antioxidant potential of seminal plasma. The relationship between BPA levels, Zn concentration and Zn/Cu ratio with human sperm parameters is still unclear. In this study, we evaluated correlation between BPA concentration, Zn concentration and ratio of Zn/Cu in seminal plasma in relation to sperm concentration, number of spermatozoa, total motility, progressive motility, morphology and DNA fragmentation. A total of 123 nonsmoking patients aged 18 - 45 (average 28) years with varicocele were included in this prospective study from 2019 to 2021. All the men signed an informed consent and agreed with analyses. Ethical Committee of University Hospital Brno approved analyses. Seminal plasma was collected after centrifugation. BPA was determined by the LC-MS/MS method (liquid chromatography-tandem mass spectrometry). The concentrations of Zn and Cu were measured by inductively coupled plasma mass spectrometry (Agilent 8900 ICP-MS/MS). Statistical evaluation was performed using Spearmen test (p 1/4 0.05). The relationship between parameters was expressed by Spearman's correlation coefficient (q). Analytical examination of seminal fluids detected presence of BPA in 89 samples (72.3 % of all samples analysed with average value 0.1627 ng/ml). In 18 samples, the concentration was under the level of detection and in 16 samples under the level of quantification. Zn was detected in all 123 patients with average concentration 139.44 mg/ml. The Zn/Cu ratio was calculated with average value 1.73. Subsequently, individual parameters of spermiogram plus DNA integrity were assessed. In case of BPA, negative correlations were found between BPA concentration and total motility (p 1/4 0.010) and progressive motility (p 1/4 0.012). Similarly, positive significant correlations between Zn/Cu ratio and total motility (p 1/4 0.032) and progressive motility (p 1/4 0.028) were found. For Zn concentration, we found positive non-significant correlation with total motility (p 1/4 0.144) or progressive motility (p 1/4 0.147). There were non-significant correlations between the BPA, Zn/Cu ratio or Zn and other parameters. Similarly, for BPA and Zn/Cu ratio a non-significant positive correlation was found (p 1/4 0.157) and neither significant correlation was found between BPA and Zn (p 1/4 0.452). In several cases we observed normal progressive and total motility in patients with high BPA concentrations together with high Zn/Cu ratio which probably has a protecting effect from the high BPA levels. Limitations, reasons for caution: A limitation for this study is the final number of patients analysed in this study, which slightly reduced the power of statistical analysis. Currently, more analyses focused on detection of BPA in seminal plasma are being carried out in order to obtain sufficiently larger data set. In this study, BPA was detected in 72% of analysed samples. Our first results indicate a higher sensitivity of spermatozoa to BPA presence in seminal fluid, affecting mainly their motility. Interestingly, a higher Zn/Cu ratio is potent to reduce partially the negative impact of BPA on human spermatozoa.
ER  -

Basic information
Original name	Correlation between Zn/Cu ratio and Bisphenol A in seminal plasma in men with varicocele.
Name in Czech	Korelace mezi poměrem ZN/Cu a bisfenolem A v seminální plazmě u mužů s varikokélou
Authors	JEŠETA, Michal, Jana NAVRÁTILOVÁ, Jiří KALINA, Lenka MEKIŇOVÁ, Jana ŽÁKOVÁ, Pavel VENTRUBA and Igor CRHA.
Edition	38th Hybrid Annual Meeting of the ESHRE, 2022.

Other information
Original language	English
Type of outcome	Conference abstract
Country of publisher	Czech Republic
Confidentiality degree	is not subject to a state or trade secret
WWW	URL
Impact factor	Impact factor: 6.100
Organization unit	Faculty of Medicine
ISSN	0268-1161
Keywords in English	Bisphenol A; spermiogram parameters; varicocele
Tags	International impact
Changed by	Changed by: Mgr. Alžběta Nagyová, učo 52254. Changed: 27/7/2023 12:23.

Abstract

Is there a relationship between concentration of Bisphenol A (BPA), Zn contents and Zn/Cu ratio in seminal fluid and spermiogram parameters in men with varicocele? Our results document correlations between BPA, Zn/Cu and sperm motility. We found that the negative effect of BPA can be attenuated by higher Zn/Cu levels. A range of environmental factors including organic endocrine disruptors affects spermatogenesis. Bisphenols are endocrine disruptors often used in production of plastics. BPA is an endocrine disruptor with negative effects on reproduction. Presence of BPA also induces production of reactive oxygen species with negative impact on spermatogenesis. Zinc and copper are trace elements important for human spermatogenesis. Zn and especially the ratio of Zn/Cu are good parameters of the antioxidant potential of seminal plasma. The relationship between BPA levels, Zn concentration and Zn/Cu ratio with human sperm parameters is still unclear. In this study, we evaluated correlation between BPA concentration, Zn concentration and ratio of Zn/Cu in seminal plasma in relation to sperm concentration, number of spermatozoa, total motility, progressive motility, morphology and DNA fragmentation. A total of 123 nonsmoking patients aged 18 - 45 (average 28) years with varicocele were included in this prospective study from 2019 to 2021. All the men signed an informed consent and agreed with analyses. Ethical Committee of University Hospital Brno approved analyses. Seminal plasma was collected after centrifugation. BPA was determined by the LC-MS/MS method (liquid chromatography-tandem mass spectrometry). The concentrations of Zn and Cu were measured by inductively coupled plasma mass spectrometry (Agilent 8900 ICP-MS/MS). Statistical evaluation was performed using Spearmen test (p 1/4 0.05). The relationship between parameters was expressed by Spearman's correlation coefficient (q). Analytical examination of seminal fluids detected presence of BPA in 89 samples (72.3 % of all samples analysed with average value 0.1627 ng/ml). In 18 samples, the concentration was under the level of detection and in 16 samples under the level of quantification. Zn was detected in all 123 patients with average concentration 139.44 mg/ml. The Zn/Cu ratio was calculated with average value 1.73. Subsequently, individual parameters of spermiogram plus DNA integrity were assessed. In case of BPA, negative correlations were found between BPA concentration and total motility (p 1/4 0.010) and progressive motility (p 1/4 0.012). Similarly, positive significant correlations between Zn/Cu ratio and total motility (p 1/4 0.032) and progressive motility (p 1/4 0.028) were found. For Zn concentration, we found positive non-significant correlation with total motility (p 1/4 0.144) or progressive motility (p 1/4 0.147). There were non-significant correlations between the BPA, Zn/Cu ratio or Zn and other parameters. Similarly, for BPA and Zn/Cu ratio a non-significant positive correlation was found (p 1/4 0.157) and neither significant correlation was found between BPA and Zn (p 1/4 0.452). In several cases we observed normal progressive and total motility in patients with high BPA concentrations together with high Zn/Cu ratio which probably has a protecting effect from the high BPA levels. Limitations, reasons for caution: A limitation for this study is the final number of patients analysed in this study, which slightly reduced the power of statistical analysis. Currently, more analyses focused on detection of BPA in seminal plasma are being carried out in order to obtain sufficiently larger data set. In this study, BPA was detected in 72% of analysed samples. Our first results indicate a higher sensitivity of spermatozoa to BPA presence in seminal fluid, affecting mainly their motility. Interestingly, a higher Zn/Cu ratio is potent to reduce partially the negative impact of BPA on human spermatozoa.

PrintDisplayed: 23/7/2024 13:37

Correlation between Zn/Cu ratio and Bisphenol A in seminal plasma in men with varicocele.

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