Mechanical-induced bone remodeling does not depend on Piezo1 in
dentoalveolar hard tissue

J 2023

Mechanical-induced bone remodeling does not depend on Piezo1 in dentoalveolar hard tissue

NOTTMEIER, Cita, Josef LAVICKÝ, Marcos GONZÁLEZ LÓPEZ, Sarah KNAUTH, Baerbel KAHL-NIEKE et. al.

Basic information

Original name

Mechanical-induced bone remodeling does not depend on Piezo1 in dentoalveolar hard tissue

Authors

NOTTMEIER, Cita, Josef LAVICKÝ (203 Czech Republic, belonging to the institution), Marcos GONZÁLEZ LÓPEZ (724 Spain, belonging to the institution), Sarah KNAUTH, Baerbel KAHL-NIEKE, Michael AMLING, Thorsten SCHINKE, Jill HELMS, Jan KŘIVÁNEK (203 Czech Republic, belonging to the institution), Till KOEHNE (guarantor) and Julian PETERSEN

Edition

Scientific Reports, Berlin, NATURE RESEARCH, 2023, 2045-2322

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10605 Developmental biology

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.600 in 2022

RIV identification code

RIV/00216224:14110/23:00134176

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1038/s41598-023-36699-9

UT WoS

001007856900061

Keywords in English

Mechanical-induced bone remodeling; Piezo1; dentoalveolar hard tissue

Abstract

V originále

Mechanosensory ion channels are proteins that are sensitive to mechanical forces. They are found in tissues throughout the body and play an important role in bone remodeling by sensing changes in mechanical stress and transmitting signals to bone-forming cells. Orthodontic tooth movement (OTM) is a prime example of mechanically induced bone remodeling. However, the cell-specific role of the ion channels Piezo1 and Piezo2 in OTM has not been investigated yet. Here we first identify the expression of PIEZO1/2 in the dentoalveolar hard tissues. Results showed that PIEZO1 was expressed in odontoblasts, osteoblasts, and osteocytes, while PIEZO2 was localized in odontoblasts and cementoblasts. We therefore used a Piezo1(floxed/floxed) mouse model in combination with Dmp1(cre) to inactivate Piezo1 in mature osteoblasts/cementoblasts, osteocytes/cementocytes, and odontoblasts. Inactivation of Piezo1 in these cells did not affect the overall morphology of the skull but caused significant bone loss in the craniofacial skeleton. Histological analysis revealed a significantly increased number of osteoclasts in Piezo1(floxed/floxed);Dmp1(cre) mice, while osteoblasts were not affected. Despite this increased number of osteoclasts, orthodontic tooth movement was not altered in these mice. Our results suggest that despite Piezo1 being crucial for osteoclast function, it may be dispensable for mechanical sensing of bone remodeling.

Links

GA22-02794S, research and development project

Name: Mechanorecepce jako mechanizmus řídící odontogenezi napříč obratlovci

Investor: Czech Science Foundation

Citovat

NOTTMEIER, Cita, Josef LAVICKÝ, Marcos GONZÁLEZ LÓPEZ, Sarah KNAUTH, Baerbel KAHL-NIEKE, Michael AMLING, Thorsten SCHINKE, Jill HELMS, Jan KŘIVÁNEK, Till KOEHNE and Julian PETERSEN. Mechanical-induced bone remodeling does not depend on Piezo1 in dentoalveolar hard tissue. Scientific Reports. Berlin: NATURE RESEARCH, 2023, vol. 13, No 1, p. 1-9. ISSN 2045-2322. Available from: https://dx.doi.org/10.1038/s41598-023-36699-9.

@article{2300517,
   author = {Nottmeier, Cita and Lavický, Josef and González López, Marcos and Knauth, Sarah and KahlandNieke, Baerbel and Amling, Michael and Schinke, Thorsten and Helms, Jill and Křivánek, Jan and Koehne, Till and Petersen, Julian},
   article_location = {Berlin},
   article_number = {1},
   doi = {http://dx.doi.org/10.1038/s41598-023-36699-9},
   keywords = {Mechanical-induced bone remodeling; Piezo1; dentoalveolar hard tissue},
   language = {eng},
   issn = {2045-2322},
   journal = {Scientific Reports},
   title = {Mechanical-induced bone remodeling does not depend on Piezo1 in dentoalveolar hard tissue},
   url = {https://www.nature.com/articles/s41598-023-36699-9},
   volume = {13},
   year = {2023}
}

TY  - JOUR
ID  - 2300517
AU  - Nottmeier, Cita - Lavický, Josef - González López, Marcos - Knauth, Sarah - Kahl-Nieke, Baerbel - Amling, Michael - Schinke, Thorsten - Helms, Jill - Křivánek, Jan - Koehne, Till - Petersen, Julian
PY  - 2023
TI  - Mechanical-induced bone remodeling does not depend on Piezo1 in dentoalveolar hard tissue
JF  - Scientific Reports
VL  - 13
IS  - 1
SP  - 1-9
EP  - 1-9
PB  - NATURE RESEARCH
SN  - 20452322
KW  - Mechanical-induced bone remodeling
KW  - Piezo1
KW  - dentoalveolar hard tissue
UR  - https://www.nature.com/articles/s41598-023-36699-9
N2  - Mechanosensory ion channels are proteins that are sensitive to mechanical forces. They are found in tissues throughout the body and play an important role in bone remodeling by sensing changes in mechanical stress and transmitting signals to bone-forming cells. Orthodontic tooth movement (OTM) is a prime example of mechanically induced bone remodeling. However, the cell-specific role of the ion channels Piezo1 and Piezo2 in OTM has not been investigated yet. Here we first identify the expression of PIEZO1/2 in the dentoalveolar hard tissues. Results showed that PIEZO1 was expressed in odontoblasts, osteoblasts, and osteocytes, while PIEZO2 was localized in odontoblasts and cementoblasts. We therefore used a Piezo1(floxed/floxed) mouse model in combination with Dmp1(cre) to inactivate Piezo1 in mature osteoblasts/cementoblasts, osteocytes/cementocytes, and odontoblasts. Inactivation of Piezo1 in these cells did not affect the overall morphology of the skull but caused significant bone loss in the craniofacial skeleton. Histological analysis revealed a significantly increased number of osteoclasts in Piezo1(floxed/floxed);Dmp1(cre) mice, while osteoblasts were not affected. Despite this increased number of osteoclasts, orthodontic tooth movement was not altered in these mice. Our results suggest that despite Piezo1 being crucial for osteoclast function, it may be dispensable for mechanical sensing of bone remodeling.
ER  -

NOTTMEIER, Cita, Josef LAVICKÝ, Marcos GONZÁLEZ LÓPEZ, Sarah KNAUTH, Baerbel KAHL-NIEKE, Michael AMLING, Thorsten SCHINKE, Jill HELMS, Jan KŘIVÁNEK, Till KOEHNE and Julian PETERSEN. Mechanical-induced bone remodeling does not depend on Piezo1 in dentoalveolar hard tissue. \textit{Scientific Reports}. Berlin: NATURE RESEARCH, 2023, vol.~13, No~1, p.~1-9. ISSN~2045-2322. Available from: https://dx.doi.org/10.1038/s41598-023-36699-9.

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